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141.
Isabell Waldmann Fereydoun Vakilzadeh 《Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete》1997,48(9):666-670
Zusammenfassung
Allergische Reaktionen auf Farbstoffe in T?towierungen sind vergleichsweise selten. In der Mehrzahl sind es Reaktionen auf
die roten Farbstoffe. W?hrend früher haupts?chlich Quecksilberverbindungen (Zinnober) und Cadmiumsulfid dafür verantwortlich
waren, stehen heute die synthetischen anorganischen Azofarbstoffe im Vordergrund. Es wird ein 42j?hriger Mann mit allergischer
Reaktion in seinen roten T?towierungsanteilen vorgestellt. Histologisch imponierte eine chronisch verlaufende granulomat?se,
teils fibrosierende Entzündung mit transfollikul?rer Elimination von Pigmentgranula. Klinisch zeigte sich im Verlauf spontan
eine partielle Rückbildung der entzündlichen Ver?nderungen unter gleichzeitiger Depigmentierung und Vernarbung der Haut. Als
T?towierungsfarbstoff wurde ein aromatischer Azofarbstoff nachgewiesen. Neben der positiven Hauttestreaktion auf diesen Farbstoff
zeigte der Patient zus?tzlich eine positive Testreaktion auf den Textilfarbstoffkoppler Napthol AS.
Eingegangen am 23. August 1996 Angenommen am 15. November 1996 相似文献
142.
CX3CR1‐dependent recruitment of mature NK cells into the central nervous system contributes to control autoimmune neuroinflammation
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Laura Hertwig Isabell Hamann Silvina Romero‐Suarez Jason M. Millward Rebekka Pietrek Coralie Chanvillard Hanna Stuis Karolin Pollok Richard M. Ransohoff Astrid E. Cardona Carmen Infante‐Duarte 《European journal of immunology》2016,46(8):1984-1996
Fractalkine receptor (CX3CR1)‐deficient mice develop very severe experimental autoimmune encephalomyelitis (EAE), associated with impaired NK cell recruitment into the CNS. Yet, the precise implications of NK cells in autoimmune neuroinflammation remain elusive. Here, we investigated the pattern of NK cell mobilization and the contribution of CX3CR1 to NK cell dynamics in the EAE. We show that in both wild‐type and CX3CR1‐deficient EAE mice, NK cells are mobilized from the periphery and accumulate in the inflamed CNS. However, in CX3CR1‐deficient mice, the infiltrated NK cells displayed an immature phenotype contrasting with the mature infiltrates in WT mice. This shift in the immature/mature CNS ratio contributes to EAE exacerbation in CX3CR1‐deficient mice, since transfer of mature WT NK cells prior to immunization exerted a protective effect and normalized the CNS NK cell ratio. Moreover, mature CD11b+ NK cells show higher degranulation in the presence of autoreactive 2D2 transgenic CD4+ T cells and kill these autoreactive cells more efficiently than the immature CD11b? fraction. Together, these data suggest a protective role of mature NK cells in EAE, possibly through direct modulation of T cells inside the CNS, and demonstrate that mature and immature NK cells are recruited into the CNS by distinct chemotactic signals. 相似文献
143.
Results of a 2‐arm,phase 2 clinical trial using post‐transplantation cyclophosphamide for the prevention of graft‐versus‐host disease in haploidentical donor and mismatched unrelated donor hematopoietic stem cell transplantation
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![点击此处可从《Cancer》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Sameh Gaballa MD MSc Isabell Ge Riad El Fakih MD Jonathan E. Brammer MD Piyanuch Kongtim MD Ciprian Tomuleasa MD Sa A. Wang MD Dean Lee MD PhD Demetrios Petropoulos MD Kai Cao MS MD Gabriela Rondon MD Julianne Chen BS Aimee Hammerstrom PharmD Lindsey Lombardi PharmD Gheath Alatrash DO PhD Martin Korbling MD Betul Oran MD Partow Kebriaei MD Sairah Ahmed MD Nina Shah MD Katayoun Rezvani MD PhD David Marin MD DM MSc Qaiser Bashir MD Amin Alousi MD Yago Nieto MD PhD Muzaffar Qazilbash MD Chitra Hosing MD Uday Popat MD Elizabeth J. Shpall MD Issa Khouri MD Richard E. Champlin MD Stefan O. Ciurea MD 《Cancer》2016,122(21):3316-3326
144.
Strassmann G Braun I Kress O Richter D Neidel HO Klose KJ An H Vogel B Rose F Engenhart-Cabillic R 《International journal of radiation oncology, biology, physics》2006,66(2):576-582
BACKGROUND: This study is situated in the area of measuring set-up accuracy and time periods of single-session extracranial radiotherapy (SSRT) for simple-shaped targets (e.g., spherical or rotational symmetrical) definitively located in the peripheral lung. METHODS AND MATERIALS: After adaptation of the stereotactic body frame, the patient has to remain in the vacuum pillow during planning computed tomography (CT), fast three-dimensional (3-D) treatment planning, and direct irradiation after verification. Fast preplanning is performed by using virtual simulation software to accelerate the method. RESULTS: In our new procedure, SSRT is applied in approximately 1.5 h. The mean setup accuracy vector was 2.4+/-0.7 mm in the range of 1.34 to 4 mm. Mean intrafractional patient movement in the stereotactic body frame before and after radiation was 0.70 mm+/-0.5 mm and 0.76+/-0.76 mm in the range of 0 to 2.8 mm. Mean time period steps were measured at (1) planning CT with 3-D treatment planning: 76+/-12 min; (2) irradiation and verification: 33+/-7 min; and (3) complete procedure duration: 109+/-11 min (range, 89-169). CONCLUSIONS: The main difference between the positioning technique of SSRT and that of conventional extracranial radiosurgery is the tighter patient fixation, which guarantees minimal patient movement. The main advantages are procedure acceleration and omission of CT simulation. SSRT is a preliminary stage of real-time treatment. 相似文献
145.
Allgeier C Kämmerle-Hofrichte I Braun S Heimann K Lauffer-Spindler B Leinweber R Scherzer A Schulz K Thiele K Wulfgramm H 《Pflege》2005,18(6):373-380
The study "determining the support needs of patients who live at home following a stroke" was chosen because stroke is (one of) the most significant causes of permanent handicap in the elderly. (This project was developed as part of the studies for the degree of Nurse Manager/Teacher:) The results are based on qualitative and quantitative data collected from focus groups and a standardised questionnaire. Due to the small number of participants (57) the study cannot be regarded as representative. Typical key issues such as "going out of the house" were highlighted in the standard questionnaires. The interviews which were evaluated using the Mayring technique give an impression of themes, such as reduced tolerance levels and (greater) increased anxiety, which together with the physical burden dominate their daily life. It was apparent that the persons concerned have a general lack of understanding of their condition. The most significant results of the study are presented together with life enhancing recommendations. 相似文献
146.
Mathias Bergmann Anke Post Isabell Rittel Ingo Bechmann R. Nitsch 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1997,117(1):80-86
Expression of the synaptic vesicle protein synaptophysin was studied in lesion-induced sprouting neurons of the contralateral
entorhinal cortex and in the contralateral dentate gyrus using immunocytochemistry at the light- and electron-microscopic
level. Perikaryal immunoreactivity for synaptophysin was found between 8 and 10 days postlesion. Light microscopy revealed
that synaptophysin immunostaining was present in almost all neurons of layers II and III of the contralateral medial entorhinal
cortex. These neurons give rise to the sprouting, crossed temporodentate pathway. In addition, some hilar neurons of the contralateral
dentate gyrus, which are the parent cells of sprouting commissural fibers, were immunostained for synaptophysin. Transient
immunostaining for synaptophysin was observed within cell bodies and dendrites. Additionally, the cell bodies were outlined
by immunoreactive puncta, identified by electron microscopy as nerve terminals. Our results revealed that sprouting neurons
express the major synaptic vesicle protein synaptophysin during reactive synaptogenesis in a pattern that reflects biosynthesis
and sorting of this protein as seen in developing neurons during synapse formation.
Received: 13 November 1996 / Accepted: 3 June 1997 相似文献
147.
Ultrasound-accelerated formalin fixation of tissue improves morphology, antigen and mRNA preservation. 总被引:1,自引:0,他引:1
Wei-Sing Chu Bungo Furusato Kondi Wong Isabell A Sesterhenn Fathollah K Mostofi Min Qi Wei Zhenqing Zhu Susan L Abbondanzo Qi Liang 《Modern pathology》2005,18(6):850-863
Formalin fixation and paraffin embedding are conventional tissue preservation and processing methods used for histologic diagnosis in over 90% of cases. However, formalin fixation has three disadvantages: (1) slow fixation (16-24 h) hinders intraoperative decision making, (2) slow quenching of enzymatic activity causes RNA degradation, and (3) extensive molecule modification affects protein antigenicity. Applying high-frequency, high-intensity ultrasound to the formalin fixative cuts fixation time to 5-15 min. Fixation of various tissues such as lymph node, brain, breast, and prostate suggests that, compared to the conventional method, implementation of ultrasound retains superior and more uniform tissue morphology preservation. Less protein antigenicity is altered so that rapid immunohistochemical reactions occur with higher sensitivity and intensity, reducing the need for antigen retrieval pretreatment. Better RNA preservation results in stronger signals in in situ hybridization and longer RNA fragments extracted from fixed tissues, probably due to rapid inhibition of endogenous RNase activity. Molecules extracted from ultrasound-fixed tissues are of greater integrity and quantity compared to conventionally fixed tissues, and thus better support downstream molecular analyses. Overall, ultrasound-facilitated tissue preservation can provide rapid and improved morphological and molecular preservation to better accommodate both traditional and molecular diagnoses. 相似文献
148.
John J. Bauer Isabell A. Sesterhenn F.K. Mostofi David G. McLeod Shiv Srivastava Judd W. Moul 《The Journal of urology》1996,156(4):1511-1516
Purpose
The tumor suppressor gene (TSG) p53 and the proto-oncogene bcl-2 have been shown to be involved in the regulation of cell growth and apoptosis and have been implicated in hormone refractory prostate cancer (PC) and poor prognosis. The goal of this study was to determine the clinical utility of the presence of p53 and bcl-2 immunohistochemical (IHC) protein in the primary tumor as predictors of disease progression following radical prostatectomy (RP).Materials and Methods
The expression of p53 and bcl-2 was evaluated in archival paraffin-embedded RP specimens from 175 patients followed from 1 to 9 years (mean = 4.6 years) and correlated with stage, grade, race and serologic (PSA) recurrence following surgery.Results
Overexpression of bcl-2 was noted in 47 of 175 (26.9 percent) patients; these patients had a significantly higher 5-year failure rate than those who did not overexpress bcl-2 (67.0 percent versus 30.7 percent). Expression of p53 was noted in 114 of 175 (65.1 percent) patients with a 5-year failure rate of 51.1 percent compared with a 5-year failure rate of only 22 percent in p53 negative patients. When expression rates for p53 and bcl-2 were combined, the 5-year failure rate was 75.3 percent. Conversely, when both p53 and bcl-2 IHC staining were negative, the 5-year failure rate was 20.4 percent. Univariate Kaplan-Meier analysis showed a statistically significant difference between p53 and bcl-2 positive and negative patients (p less than 0.001). Multivariate Cox Regression Analysis with backward elimination controlling for age, race, stage and grade showed both p53 (p = .0185) and bcl-2 (p = .044) to be independent predictors of disease-free survival.Conclusion
p53 and bcl-2 appear to be important biomarkers that predict recurrence in clinically localized PC after RP. 相似文献149.
Preneoplastic non-papillary lesions and conditions of the urinary bladder: an update based on the Ancona International Consultation 总被引:1,自引:0,他引:1
Lopez-Beltran A Cheng L Andersson L Brausi M de Matteis A Montironi R Sesterhenn I van det Kwast KT Mazerolles C 《Virchows Archiv : an international journal of pathology》2002,440(1):3-11
BACKGROUND AND AIMS: This paper summarizes the work done by the members of the Committee no. 2 at the International Consultation on the Diagnosis of Non-Invasive Urothelial Neoplasms held in Ancona, Italy (11-12 May 2001). The committee members discussed and reached consensus regarding the optimal contemporary diagnosis and classification of the preneoplastic non-papillary lesions of the urothelium. An important objective was to promote a precise terminology and to use it consistently in daily practice in pathology and urology. RESULTS AND CONCLUSIONS: The result of the meeting is represented by a refined classification of the non-papillary intraepithelial lesions and conditions of the urothelium. This classification includes epithelial abnormalities (reactive urothelial atypia and flat urothelial hyperplasia), presumed preneoplastic lesions and conditions (keratinizing squamous and glandular metaplasia, and malignancy-associated cellular changes), as well as preneoplastic (dysplasia) and neoplastic non-invasive (carcinoma in situ) lesions. Each of these lesions is defined with strict morphological criteria in order to provide more accurate information to urologists in managing patients. 相似文献
150.
Malene Stutzki Evelyn Jahns Magis Matthew Mandapathil Isabell Diogo Jochen Alfred Werner 《Acta oto-laryngologica》2015,135(12):1337-1343
Conclusion: Cone Beam Computed Tomography (CBCT) can be widely used in imaging of bony structures of the anterior and lateral skull base. Significant differences of applied dosages result from the different protocols of the various indications. Objectives: CBCT is increasingly being used in head and neck imaging. Until now, no precise knowledge about its dedicated usage existed. Methods: All CBCT of 2012 and 2013 were analysed with regard to the technical parameters, the performance, and the indication for imaging. Results: In total, 1862 patients were examined in 2012 and 2013. The top eight indications of the anterior skull were (1) chronic rhinosinusitis with disturbed nasal breathing (30.3%); (2) chronic rhinosinusitis (17.6%); (3) midfacial traumatology (13.7%); (4) disturbed nasal breathing (12.8%); (5) acute rhinosinusitis (7.9%); (6) polyposis nasi (6.3%); (7) search for focus (3.9%); and (8) persistent rhinorrhea (1.2%). For the lateral skull base, the top eight were (1) control after cochlear implantation (28.4%); (2) cholesteatoma (19.7%); (3) visualization of ear anatomy (8.7%); (4) chronic otitis media mesotympanalis (6.3%); (5) conductive hearing loss (5.1%); (6) suspected mastoiditis (4.8%); (7) pathology of external auditory canal (4.8%); and (8) otosclerosis (3.3%). Applied dosage for the anterior skull base was significantly lower than for the lateral skull base (2.90 mGy vs 5.92 mGy, p < 0.05); 2.4% and 3.6% of patients’ images, respectively, had to be repeated. 相似文献