Paracrine effects of hepatocyte growth factor/scatter factor on non-small-cell lung carcinoma cell lines.

We have studied the mitogenic, motogenic and morphogenic effects of hepatocyte growth factor (HGF), also known as scatter factor (SF), on 15 non-small-cell lung carcinoma (NSCLC) cell lines that have had their ras genotype determined. HGF/SF stimulated proliferation in only three cell lines and exerted no mitogenic activity on six lines. The growth of the remaining six lines was inhibited. The mitogenic effects were not related to the ras genotype of these cell lines, but the inhibitory effect was more commonly observed in cell lines with relatively high levels of Met/HGF receptor (HGFR) expression. HGF/SF induced or enhanced both scatter activity on monolayer culture and single-cell invasion in collagen gels in approximately half of these cell lines. Although the ras genotype of tumour cells did not influence the HGF/SF-induced motogenic activity, cell lines with the mutant ras genotype more commonly demonstrated a spontaneous motogenic activity than those with the wild-type ras genotype. When tumour cells were grown in collagen gels, HGF/SF induced irregular branching extensions of cell aggregates formed by five out of eight adenocarcinoma cell lines, but significant lumen morphogenesis was distinctly absent. The presence of autocrine HGF/SF loop in these tumour cell lines did not influence their spontaneous or HGF/SF-induced mitogenic, motogenic or morphogenic activities. Overall, our data suggest that stimulation of cell motility, rather than proliferation or differentiation, is the predominant paracrine effect of HGF/SF on NSCLC cells in vitro.     

进口化学药品说明书的基本要素

药品说明书是药品获批准上市后的重要证明文件之一，是对被批准的适应症、用法、用量等有关用药重要信息的详细说明，是指导医患用药的关键依据。在《中华人民共和国药品管理法》、《药品注册管理办法》（试行）以及国家食品药品监督管理局局23号令等法律、法规和条例中，对药品说明书及相关内容明确了其要求和责任等，这对认识说明书的作用和地位，并且对其进行制订和核准都起着重要的指导作用。     

Mortality: What Are the Roles of Risk Factor Prevalence,Screening, and Use of Recommended Treatment?

CONTEXT: Despite advances in early detection and prevention of cervical cancer, women living in rural areas, and particularly in Appalachia, the rural South, the Texas/Mexico border, and the central valley of California, have had consistently higher rates of cervical cancer mortality than their counterparts in other areas during the past several decades. METHODS: This paper reviews the published literature from 1966 to July 2002 to assess three potential pathways underlying this excess mortality--high human papilloma virus (HPV) prevalence, lack of or infrequent screening and advanced disease at diagnosis, and under-use of recommended treatment and shorter survival. FINDINGS: Living in rural areas may impose barriers to cervical cancer control, including lack of transportation and medical care infrastructures. Population characteristics that place women at greater risk for developing and dying from cervical cancer, such as low income, lack of health insurance, and physician availability, are concentrated in rural areas. Published data, however, are insufficient to identify the key reasons for the observed mortality patterns. CONCLUSIONS: At this time, given the lack of definitive evidence in the published literature, decisions about priorities in areas with high rates of cervical cancer mortality will depend on knowledge of current levels of screening, incidence, and stage distribution; and service delivery infrastructures, resources, and acceptability of interventions to the target population.     

柴莪退热栓治疗急性病毒性上呼吸道感染临床研究

目的观察柴莪退热栓治疗急性病毒性上呼吸道感染中医辨证属卫分证、卫气同病证的临床疗效,并探讨外感高热卫分证、卫气同病证证候与疗效间的关系.方法将患者 80例随机分为治疗组与对照组,分别予柴莪退热栓和消炎痛栓塞肛,并进行安全性评价.结果两组总体疗效相当,且未发现明显毒副作用.结论 柴莪退热栓治疗急性病毒性上呼吸道感染疗效满意.     

医院设备购置中的供应商选择

供应商选择在医院设备采购中越来越重要。本文构建了医疗设备的供应商评价体系，然后应用模糊综合评判方法建立了供应商评价模型，最后给出算例予以说明。     

内蒙古西部地区城乡蒙汉族女生经前准备情况

目的了解城乡蒙汉族中小学女生的经前准备情况,为开展青春期健康教育提供科学依据.方法采用分层整群抽样的方法,对蒙汉族中小学女生6 298名进行经前准备情况调查.结果城乡蒙汉族中小学女生的经前教育情况城市好于农村,汉族好于蒙族,但总体情况不容乐观.对月经感到紧张忧虑的女生其月经失调、痛经和经前期紧张综合征的发生率均较高.结论应对中小学女生进行正确的、恰当的经前教育,以确保其身心健康.     

硅油支持眼的人工虹膜膈植入

目的 总结硅油支持眼的人工虹膜膈植入的临床效果。方法 回顾性研究11例硅油支持眼人工虹膜膈植入手术适应症，手术效果及手术并发症。结果 11例人工虹膜膈植入眼原发眼病中，7例(7／11，64％)为严重外伤眼，2例(2／11，18％)为增埴期糖尿病性视网膜病变合并牵引性视网膜脱离眼，1例(1／11，9％)为严重PVR后复发性视网膜脱离眼，1例(1／11。9％)为Coats病眼；7例(／11，64％)人工虹膜膈有效地防止了硅油接触角膜，11例人工虹膜膈植入眼。均未引起视力的进一步下降；其手术后并发症主要为低眼压和人工虹膜膈表面的纤维膜形成。结论 硅油支持眼的开放性人工虹膜膈植入可通过自然隔断，建立房水循环来维持眼压，有效地防止硅油接触角膜引起并发症。     

顽固高眼压持续状态的急性闭角型青光眼急诊手术26例

目的:探讨急性闭角型青光眼高眼压持续状态下的急诊手术治疗。(急性闭角型青光眼高眼压持续状态:急性闭角型青光眼眼压高达60mmHg应用脱水缩瞳封闭等方法治疗眼压波动在6mmHg者谓之)。方法:对26例(28眼)急性闭角型青光眼手术治疗中,18眼应用前房穿刺临时降低眼压,10眼巩膜瓣完成后于巩膜瓣下角巩缘切开缓慢入出房水临时降低眼压后皆按常规完成青光眼小梁切除术;术中并发症主要为3眼前房出血;术后并发症为4眼前房消失脉络膜脱离。以上并发症经临床药物、脉络膜放液前房注入Henlon等治愈。结果:术后眼压全部恢复至21mmHg以下。术后1周检查眼底,7眼有视网膜片状出血,经治疗3月后出血吸收呈斑块状改变。结论:对于急性闭角型青光眼高眼压持续状态患者,在应用药物治疗无效时,为保护患者有用视力,手术介入是积极有效的;术中和术后并发症通过药物和其它治疗可以治愈,故急诊手术应是首选方法。     

恶性肿瘤放化疗并发带状疱疹27例临床分析

目的　接受放化疗的恶性肿瘤患者并发带状疱疹的临床分析。方法　回顾性分析 1998年 1月至2 0 0 3年 11月的 2 7例恶性肿瘤放化疗并发带状疱疹的住院患者的临床资料、治疗方法、随访结果。结果　全组死亡 16例 (5 9% ) ,其中 11例 (41% )死于发病后 0 .5 a内 ,3例 (11% )死于发病后 1a内 ,2例 (7% )死于发病后 3a内。死亡的 16例患者中 ,播散型患者 7例 ,局限型患者 9例。结论　对恶性肿瘤放化疗并发带状疱疹应有足够的认识 ,以期早发现、早诊断、早治疗 ,减少不必要的并发症的发生     

卵巢上皮癌组织中MT1-MMP的表达及意义

目的　探讨膜型基质金属蛋白酶 - 1(membranetype - 1matrixmetalloproteinase,MT1-MMP)在卵巢上皮癌组织中的表达及其与肿瘤生物学行为和预后的关系。方法　用免疫组化SP法检测 5 6例卵巢上皮癌组织、 15例卵巢良性肿瘤组织以及 10例正常卵巢组织中MT1-MMP蛋白的表达。结果　 6 7 9%的卵巢上皮癌组织MT1-MMP表达阳性 ,卵巢良性肿瘤组织很少表达 (4/ 15 ) ,正常卵巢组织中几乎不表达 (1/ 10 ) ,差异有显著性 (P <0 0 1)。MT1-MMP的表达水平与临床分期、组织学分级以及淋巴结转移密切相关。在单因素生存分析中 ,MT1-MMP的表达与患者预后不良有关。但是 ,在多因素分析中 ,MT1-MMP的表达并不是一个相对独立的预后因素。结论　①MT1-MMP在卵巢上皮癌中异常高表达 ;②MT1-MMP表达促进卵巢癌的侵袭和转移 ,可作为判断卵巢癌恶性表型的有用指标 ;③MT1-MMP的阳性表达与患者不良预后有关。