癌症病人主要照顾者沟通认知现状调查

[目的]探讨癌症病人主要照顾者沟通认知现状。[方法]对住院癌症病人的主要照顾者进行沟通认知问卷调查。[结果]在主要照顾者的沟通认知项目中得分最高的是"沟通可给予病人情感支持",得分最低的是"沟通可使病人正确认识疾病"。不同年龄、文化程度、有无医护沟通指导的主要照顾者的沟通认知存在差异。[结论]主要照顾者对沟通在帮助病人认识疾病方面的重要性认识不足。     

人工冬虫夏草联合糖皮质激素对大鼠肺纤维化的干预作用

目的:探讨人工冬虫夏草(虫草菌液)联合糖皮质激素对博莱霉素诱导的大鼠肺纤维化的干预作用.方法:50只SD大鼠,随机分为5组:正常对照组、模型组、虫草菌液组、强的松组、虫草菌液联合强的松组(联合组).采用气管内注射博莱霉素建立模型,次日起分别给药灌胃治疗,正常组和模型组给予生理盐水.第28天处死动物,肺组织标本行HE染色及MASSON染色进行病理形态学观察、Ashcroft纤维化评分,碱水解法检测肺组织HYP含量,免疫组化检测肺组织CTGF蛋白表达,酶联免疫法检测肺泡灌洗液上清TGF-β1含量.结果:与模型组相比,虫草菌液、强的松组能够降低肺组织纤维化评分,减少肺组织胶原沉积、HYP含量,下调肺组织CTGF蛋白表达,降低BLAF中TGF-的含量;联合组分别与虫草菌液组、强的松组比较,能够进一步减少肺纤维化评分、胶原面积、HYP含量,进一步下调CTGF蛋白、TGF-蛋白表达.结论:虫草菌液、强的松对博莱霉素诱导的大鼠肺纤维化有治疗作用,且虫草菌液联合强的松能产生协同治疗效果.     

上钢社区部分居民血糖异常情况的调查与分析

目的通过对社区482例社区居民的现况调查,了解上钢社区糖尿病患病情况,从而有针对性地开展预防和干预。方法随机选择上钢社区2个居委,按5％的比例随机抽取20岁以上的居民482人,通过糖尿病现况问卷调查,统计分析现在糖尿病患者的基本情况。结果上钢社区的糖尿病患病率为17．84％,IGT患病率为7．8％。40岁以上人群是体质指数超标和血糖异常的主要患者。结论应当加强社区40岁以上人群的糖尿病筛查工作,特别是餐后血糖的筛查。     

Effects of borneol on the intestinal transport and absorption of two P-glycoprotein substrates in rats

As the most prevalent route of delivery, oral administration has the challenge of potentially low bioavailability in part because P-glycoprotein (P-gp) in the intestinal tract affects absorption. Therefore, absorption enhancers or P-gp inhibitors are strategies to solve this problem. The aim of the present study was to investigate the effects of borneol on transportation of colchicine and rhodamine123, two P-gp substrates, in rats. In vitro transportation was assessed with a diffusion chamber system with isolated rat intestines. Different concentrations of borneol (10, 40 and 80 μg/mL) were prepared in solutions with two P-gp substrates compared with blank solutions. The in vivo effects on colchicine were assessed by a pharmacokinetic study. Borneol enhanced the absorptive transport of two P-gp substrates, which was relevant to the concentration. A pharmacokinetic study showed that in the presence of borneol, a significant increase in C_max and AUC_0→8 of colchicine occurred when compared to colchicine alone. The study showed that borneol affected two P-gp substrates in the intestine, possibly by inhibiting the effects of P-gp and enhancing intestinal absorption of drugs. Therefore, borneol could be developed as a P-gp inhibitor and absorptive enhancer.     

异位妊娠260例临床分析

目的：探讨异位妊娠的发病原因及临床治疗方法.方法：回顾性分析我院2005年11月至2009年12月期间收治260例异位妊娠患者的临床资料、发病原因及治疗方法.结果：异位妊娠发病率的增加与盆腔炎、流产、IUD、输卵管病理改变有明显的相关性.结论：异位妊娠发生率逐年上升与近年来人工流产、剖宫产及盆腔炎患者的增加有关.     

Transsphenoidal surgery in a patient with acromegaly and McCune-Albright syndrome: application of neuronavigation

The McCune-Albright syndrome (MAS) is characterized by a clinical triad of polyostotic fibrous dysplasia, café-au-lait hyperpigmented macules, and hypersecretory endocrinopathies. Acromegaly is an uncommon manifestation of the endocrine disturbance associated with MAS, and the role of surgery in managing these cases has been a topic of debate. The authors present the case of a 35-year-old man with MAS who was also diagnosed with acromegaly, hyperprolactinemia, and pituitary macroadenoma. The patient had an 18-year history of fibrous dysplasia involving the right frontal bone and ribs as well as multiple endocrinopathies, but no cutaneous hyperpigmented macules. An oral glucose tolerance test demonstrated partial suppression of plasma levels of growth hormone (GH). The patient underwent transsphenoidal resection of the pituitary tumor, performed with assistance of neuronavigation, and tolerated the procedure well. After the surgery, both prolactin and GH levels returned to normal. These results suggest that neuronavigation-assisted transsphenoidal surgery can safely remove pituitary adenomas associated with MAS and successfully treat the underlying endocrine abnormalities.     

生药学实验教学新体系的探索与建立

生药学实验教学是生药学教学中的一个重要环节,坚持教学与科研相结合、课内与课外相结合,构建生药学实验教学新体系,从而培养学生综合专业素质、创新思维能力及独立工作能力。     

反相高效液相色谱测定葛根中精氨酸含量

目的:建立以反相高效液相色谱测定葛根中精氨酸含量的方法.方法:以邻苯二甲醛为柱前衍生化试剂;色谱柱为ODS HYPERSIL ClB(150mm×4.6mm,5μm),流动相为磷酸二氢钾缓冲液(pH=3.0)-甲醇(65:35),检测波长为340nm.结果:精氨酸进样量在0.1～2.0μg(r=0.999 7)范围内线性关系良好;平均回收率为99.08%,RSD=1.92%(n=6).结论:本法简便、准确、可靠,适用于葛根中精氨酸的含量测定.     

MicroRNA-125b/Lin28 pathway contributes to the mesendodermal fate decision of embryonic stem cells

MicroRNAs (miRNAs) are important regulators of cell fate decisions, while the miRNAs and their targets in the regulation of stem cell differentiation are largely unidentified. Here we report novel functions of miR-125b/Lin28 axis in the regulation of mouse embryonic stem cell (mESC) lineage specification and cardiomyocyte differentiation. With a MicroRNA Array screen, we identified a number of miRNAs significantly changed during ESC differentiation, among which miR-125b showed a marked reduction during early differentiation. The abundantly expressed miR-125b in undifferentiated mESCs was dramatically downregulated to a level hardly detected during differentiation day 3 to 5, with a concomitant upregulation of Lin28. Ectopically expressing miR-125b did not alter characteristics of undifferentiated mESCs, whereas it impaired the endoderm and mesoderm development, but not the ectoderm, and inhibited cardiomyocyte formation. We further demonstrate that miR-125b targeted the 3'-untranslated region of Lin28 and reduced the abundance of Lin28 at both mRNA and protein levels. Moreover, phenotypes of miR-125b overexpressing cells were mimicked by downregulation of Lin28 and rescued by reintroduction of Lin28. In addition, the impaired cardiogenesis in miR-125b-introduced cells was greatly recovered when mimicking endoderm environment by cultivation with the condition medium from a visceral endoderm-like cell line, END-2. These results reveal that the miR-125b/Lin28 axis is an important regulator of early lineage specification and cardiomyocyte differentiation of ESCs.