高通量测序分析重度寻常痤疮患者和健康人肠道菌群差异

目的 利用高通量测序技术分析重度寻常痤疮患者和健康人肠道菌群的差异.方法 门诊确诊的重度寻常痤疮患者10例及年龄、性别相匹配的健康人10例,分别收集粪便标本,提取细菌DNA,行16S rRNA测序鉴定微生物的种类,并进行菌群差异分析.结果 两组肠道菌群多样性未发现显著差异,仅在肠道菌群的相对丰度上发现个别具有显著差异的菌.两组标本主要由拟杆菌门、厚壁菌门、变形菌门、放线菌门组成.比较两组在门、属水平上菌群相对丰度,差异均无统计学意义;在种水平上,Blautia producta、Coprococcus eutactus在两组间差异有统计学意义.结论 重度寻常痤疮患者和健康人相比,菌群多样性指数未见明显差异.但某些细菌菌种存在显著差异.     

iTRAQ-Based Quantitative Proteomics Reveals the Energy Metabolism Alterations Induced by Chlorogenic Acid in HepG2 Cells

Epidemiological studies have suggested that coffee consumption is associated with a decrease in the risk of developing obesity and diabetes; however, the detailed mechanisms underlying these effects of coffee consumption remain poorly understood. In this study, we examined the effects of chlorogenic acid on energy metabolism in vitro. Hepatocellular carcinoma G2 (HepG2) cells were cultured in a medium containing chlorogenic acid. Chlorogenic acid increased the activity of mitochondrial enzymes, including citrate synthase, isocitrate dehydrogenase, and malate dehydrogenase (MDH), which are involved in the tricarboxylic acid (TCA) cycle. Proteome analysis using the isobaric tags for the relative and absolute quantitation (iTRAQ) method revealed the upregulation of proteins involved in the glycolytic system, electron transport system, and ATP synthesis in mitochondria. Therefore, we propose a notable mechanism whereby chlorogenic acid enhances energy metabolism, including the TCA cycle, glycolytic system, electron transport, and ATP synthesis. This mechanism provides important insights into understanding the beneficial effects of coffee consumption.     

Praseodymia–titania mixed oxide supported gold as efficient water gas shift catalyst: modulated by the mixing ratio of oxides

Modulating the active sites for controllable tuning of the catalytic activity has been the goal of much research, however, this remains challenging. The O vacancy is well known as an active site in reducible oxides. To modify the activity of O vacancies in praseodymia, we synthesized a series of praseodymia–titania mixed oxides. Varying the Pr : Ti mole ratio (2 : 1, 1 : 2, 1 : 1, 1 : 4) allows us to control the electronic interactions between Au, Pr and Ti cations and the local chemical environment of the O vacancies. These effects have been studied study by X-ray photoelectron spectroscopy (XPS), CO diffuse reflectance Fourier transform infrared spectroscopy (CO-DRIFTS) and temperature-programmed reduction (CO-TPR, H₂-TPR). The water gas shift reaction (WGSR) was used as a benchmark reaction to test the catalytic performance of different praseodymia–titania supported Au. Among them, Au/Pr₁Ti₂O_x was identified to exhibit the highest activity, with a CO conversion of 75% at 300 °C, which is about 3.7 times that of Au/TiO₂ and Au/PrO_x. The Au/Pr₁Ti₂O_x also exhibited excellent stability, with the conversion after 40 h time-on-stream at 300 °C still being 67%. An optimal ratio of Pr content (Pr : Ti 1 : 2) is necessary for improving the surface oxygen mobility and oxygen exchange capability, a higher Pr content leads to more O vacancies, however with lower activity. This study presents a new route for modulating the active defect sites in mixed oxides which could also be extended to other heterogeneous catalysis systems.

Schematic illustration of H₂O activation on the Pr-TiO_x support and the following reaction with CO in the Au–oxide interface.     

吸烟对缺血性脑血管病患者颈动脉的影响

目的 探讨长期、大量吸烟者颈动脉粥样硬化病变特点及其与缺血性脑血管病(ICVD)的关系.方法 对46例烟龄≥20年,每天吸烟≥20支的ICVD患者和40例不吸烟或偶吸少量烟的健康对照者进行颈动脉超声检测,观察并记录颈动脉内-中膜有无增厚,有无斑块,斑块数目和回声情况,测量舒张期内-中膜厚度(IMT)等.结果 吸烟的ICVD组比健康对照组斑块数目及低回声斑块检出率增加,最大IMT和平均IMT增厚(P＜0.05),斑块总积分与对照组相比差异有显著性(P＜0.01).结论 吸烟是颈动脉粥样硬化及ICVD的独立危险因素,对吸烟人群早期干预,对防止动脉粥样硬化及ICVD的发生有重要的意义.     

微血管减压术治疗面肌痉挛患者的围术期护理

目的探讨微血管减压术治疗面肌痉挛术后出现的并发症及围术期护理措施。方法统计微血管减压术治疗面肌痉挛的患者例数以及并发症发生情况,回顾性分析住院期间发生并发症的患者病历资料。结果 28例患者中,术后出现面瘫8例、听力下降3例、后组颅神经损伤1例、小脑损伤1例、脑脊液漏1例,通过有效的护理评估和干预,患者均顺利度过术后并发症高危期。结论精心的护理能减少并发症给患者造成的伤害和促进患者康复。     

Effect and mechanism of 125I radioactive particles interposed radiotherapy between organizations on lung cancer

Objective： The aim of this study was to evaluate effect and mechanism of 125I radioactive particles interposed radiotherapy between organizations on lung cancer. Methods： Fourteen cases of patients diagnosed with non-small cell lung cancer （NSCLC）, the use of the B-, CT-guided, according to preoperative imaging and treatment planning system （TPS） program for radioactive particles interposed 125I interstitial radiotherapy. Results： All patients were successfully 125I interstitial radioactive particles interposed radiotherapy. Postoperative local complete tumor remission in 9 cases, partial remission in 5 cases, the efficiency of 100%. No case of serious complications. After 3 to 4 weeks of chemotherapy after 11 cases. 4 cases of lung cancer with bone metastases, pain completely disappeared after treatment. Up to now, five cases have died due to tumor progression, survival time of 12 to 16 months. Nine cases still under follow-up observation and treatment. Conclu- sion： 125I radioactive particles interposed radiotherapy between organizations of lung cancer, simple operation, trauma, fewer complications, conformal high, high local tumor dose, efficacy, and is a supplement of modern radiotherapy techniques for the treatment of lung cancer provides a comprehensive line of the method of effective.