Recurrent corneal perforation and acute calcareous corneal degeneration in chronic graft-versus-host disease.

Keratoconjunctivitis sicca (KCS) is a common complication of graft-versus-host disease (GVHD), and may lead to corneal epithelial defect and melting. In contrast, recurrent corneal calcareous degeneration and perforation is rare. A 46-year-old woman developed chronic GVHD after bone marrow transplantation for aplastic anemia. Severe KCS with corneal melting and calcium deposits were noted in the left eye. Penetrating keratoplasty was performed because of corneal perforation, but poor re-epithelialization and calcium deposition recurred. Lamellar keratectomy and amniotic membrane transplantation (AMT) were performed, but acute calcareous degeneration developed with subsequent recurrence of corneal perforation. After regraft, AMT and tarsorrhaphy, the corneal graft remained clear for 3 months. However, breakdown of the corneal epithelium occurred 3 weeks after spontaneous separation of tarsorrhaphy. Six months later, corneal perforation recurred again along with exacerbation of GVHD. Regraft was performed, but the patient refused tarsorrhaphy and AMT. Poor re-epithelialization persisted after regraft. Corneal melting with impending corneal perforation ensued. Further corneal surgery was refused and the patient chose to undergo evisceration. This case demonstrates that the ocular complications of GVHD may be severe enough to lead to corneal perforation and calcareous degeneration that is recalcitrant to medical and surgical treatment.     

未足月胎膜早破的封闭疗法

1PPROM概况 未足月胎膜早破（preterm premature rupture of the membranes，PPROM）是指妊娠未满37周胎膜在临产前发生破裂。在所有妊娠中PPROM发生率为2％～3％，早产30％～40％由PPROM引发，PPROM孕妇中只有7．7％～9．7％的胎膜破损能够自然闭合，而60％～80％的孕妇在胎膜破裂7d内分娩，其中位潜伏期为6．6d。     

子癎前期子癎并发止血功能异常的特点与处理

止血功能包括血管壁、血小板、凝血与抗凝血的作用,后者又包括纤维蛋白溶解系统的功能。正常情况下,各部分功能平衡,保证血管内的血液呈流态;血管损伤时血栓形成以止血。正常妊娠中晚期,由于产后止血的需要,大多数凝血因子在肝脏合成增加,同时纤溶活性降低,处于生理性的高凝状态。子疒间前期、子疒间患者由于血管内皮细胞损伤,血小板活化、抗凝血酶Ⅲ消耗,使机体处于明显的血栓倾向,此种更高水平的高凝状态可导致子疒间前期严重并发症的发生,如弥散性血管内凝血(DIC)、胎儿生长受限。1子疒间前期、子疒间并发止血功能异常的特点1.1血小板…     

Molecular Characterization of Duck Hepatitis B Virus Isolated from Hubei Brown Ducks

The objective of this study was to characterize the genome structure of duck hepatitis B virus (DHBV) isolated from Hubei brown ducks. The natural carrier rate of DHBV in adult ducks from Hubei area was investigated and the DHBV DNA-positive serum screened out. The complete genome of a DHBV strain was amplified by polymerase chain reaction (PCR) and cloned into T vector and sequenced. The results showed that the carrier rate of DHBV in Hubei brown ducks was 10 %. This strain (GenBank accession number DQ276978) had a genome of 3024 nucleotides with three overlapping open reading frames encoding the surface, core and polymerase proteins respectively. Comparison of the strain with 17 DHBV strains registered in GenBank revealed a homology from 89.3 % to 93.5 % at the nucleotide level. The sequences of the structural and functional domains of these proteins were highly conserved. The strain was found to share more signature amino acids in the polymerase genes with the "Chinese" DHBV strains than those of the "Western" country strains. This finding was also corroborated by a phylogenetic tree analysis. Therefore, the DQ276978 might belong to a subtype of the Chinese DHBV strains.     

Vascular endothelial growth factor expressing mesenchymal stem cells improves cardiac function in chronic myocardial infarction in pigs

Transplantation of mesenchymal stem cells （MSCs） for myocardial reconstruction has shown promise in both animal models and human phase 1 clinical studies. Vascular endothelial growth factor （VEGF） is a strong therapeutic agent for treating ischaemia by inducing angiogenesis. The feasibility of ex vivo MSCs mediated gene transfer is documented. Matsumoto and colleagues have recently reported genetically engineered MSCs carrying VEGF165 delivery for revascularization in a model of acute myocardial infarction （MI）. The promising data from our laboratory in both angiogenesis and MSCs transplantation in cunicular heart model of acute MI have prompted us to attempt the combined and simultaneous application of the two strategies.     

Mechanism of Arsenic Trioxide Inhibiting Angiogenesis in Multiple Myeloma

Duringthelast decade,the efficacy of arsenic tri-oxide(As2O3)in both newly diagnosed and relapsedpatients with acute promyelocytic leukemia(APL)hasbeen established.And now,As2O3is used with otheragentsintreating patients with relapsed and refractorymultiple myeloma(MM)in clinical practice[1].Theinitial researches demonstratedthat As2O3couldinhibitproliferation andinduce apoptosisin MMcellsin vitroandin vivo[2,3].In addition to affecting tumorgrowth,As2O3has been showntoinhibit angiogenes…     

Expression and characterization of Gag protein of HIV-1（CN） in Pichia pastoris

To express the core protein of HIV-1 of Chinese prevalent strain （HIV-1 （CN）） in Pichia pastoris, the fulllength gag gene was inserted into the secretory expression vector pHILS1. Linearized recombinant plasmid pHILGAG by Sail was electrotransformed into the yeast strain GS115, and the yeast transformants were identified by PCR. To induce the interest protein to be expressed, the PCR positive transformants were inoculated in the medium of BMGY and BMMY, mRNA of the strain was detected by RT-PCR, and the expressed protein was analyzed by SDS-PAGE, Western blotting and thin layer scanning. mRNA （1.3 kb） was amplified by RT-PCR. SDS-PAGE and Western blotting analysis showed that the molecular mass of the expressed protein was 55 kD, which was similar to the expected value, and the expressed protein could react with McAb to HIV-1 p24. Thin layer scanning analysis demonstrated that the whole amount of the expressed protein was approximately 13 % of the soluble protein in the supernatant. The recombinant yeast had good genetic stability. The optimal expression conditions of the engineering yeast were as follows： BMMY medium, 80-90% of dissolved oxygen, 1% methanol, and 3-day-cultivation course. Gag proteins were expressed under the optimal expression condition and purified via gel filtration chromatography. The purity of the interest protein was up to 85 %. After the purified proteins were inoculated into BALB/c mice, the anti-HIV-1 antibodies in the immunized mice could be detected by Western blotting.     

中国居民代谢综合征与脑卒中的相关性研究

目的探讨中国居民代谢综合征与脑卒中的关系。方法数据来源于2002年中国居民营养与健康状况调查,选择15岁以上人群48633例,其中男性23080例,女性25553例。代谢综合征的定义参考中华医学会糖尿病分会2004年建议的标准。结果单因素logistic回归分析表明,代谢综合征与脑卒中呈正相关,OR值为5.998(95%CI4.799~7.496),多因素分析调整年龄、性别、6类地区、教育程度、中重度体力活动时间、吸烟、饮酒后代谢综合征与脑卒中仍具有统计学相关性,OR值为3.114(95%CI2.432~3.987)。代谢综合征的所有组分中,高血压与脑卒中的相关性最强,且随着代谢综合征组分在个体聚集越多(趋势性检验P<0.0001),患脑卒中的可能性越大。结论中国居民代谢综合征与脑卒中的患病有关。     

吸收性明胶海绵在鼻出血外科手术中的应用研究

目的 探讨吸收性明胶止血海绵在鼻出血、鼻外科手术止血中的临床价值.方法 将135例鼻出血、鼻外科手术后患者随机分为两组,分别应用明胶止血海绵及凡士林油纱条填塞止血,观察填塞后的疼痛程度、出血情况及术后恢复情况.结果 明胶海绵组较凡士林油纱条组填塞的头痛及鼻腔疼痛症状明显减轻,出血量减少,恢复的时间缩短.结论 吸收性明胶止血海绵较传统凡士林油纱条具有较大优势,但在特殊情况下不能取而代之.     

医院信息采集质量监控方案的设计

就医院信息系统运行过程中出现的数据质量缺陷问题，提出了实时数据质量监控和终末数据质量监控方案。该方案实施的是以标准库为基础的服务器端数据质量监控模式，该模式的优点是尽可能快地发现可疑数据，发出预警提示，反馈给有关用户，且不影响客户端数据录入程序的使用。