Orbital Fat Volumetry and Water Fraction Measurements Using T2-Weighted FSE-IDEAL Imaging in Patients with Thyroid-Associated Orbitopathy

BACKGROUND AND PURPOSE:The quantitative evaluation of orbital fat proliferation and edema and the assessment of extraocular muscles are useful for diagnosing and monitoring thyroid-associated orbitopathy. To evaluate therapy-induced quantitative changes in the orbital fat of patients with thyroid-associated orbitopathy, we performed volumetric and water fraction measurements by using T2-weighted FSE iterative decomposition of water and fat with echo asymmetry and least-squares estimation (FSE-IDEAL) imaging.MATERIALS AND METHODS:Orbital FSE-IDEAL images of 30 volunteers were acquired twice within 1 week. Nine patients with thyroid-associated orbitopathy underwent FSE-IDEAL imaging before and after methylprednisolone pulse therapy, and the treatment results were assessed by using their pre- and post-methylprednisolone pulse therapy clinical activity scores. We performed volumetric and water fraction measurements of orbital fat by using FSE-IDEAL imaging and evaluated interscan differences in the volunteers. In patients with thyroid-associated orbitopathy, we compared pre- and posttherapy orbital fat measurements and assessed the correlation between the pretherapy values and clinical activity score improvement.RESULTS:The reproducibility of results obtained by the quantitative evaluation of orbital fat in volunteers was acceptable. After methylprednisolone pulse therapy, the water fraction in the orbital fat of patients with thyroid-associated orbitopathy was significantly decreased (P < .001). There was a significant positive correlation between the pretherapy water fraction and clinical activity score improvement (right, r = 0.82; left, r = 0.79) and a significant negative correlation between the pretherapy volume and clinical activity score improvement (bilateral, r = −0.84).CONCLUSIONS:Volumetric and water fraction measurements of orbital fat by using FSE-IDEAL imaging are feasible and useful for monitoring the effects of therapy and for predicting the response of patients with thyroid-associated orbitopathy to methylprednisolone pulse therapy.

Thyroid-associated orbitopathy (TAO) is the most common extrathyroid manifestation of Graves disease. The enlargement of orbital fat and extraocular muscles in the relatively fixed volume space imposed by the bony orbit may produce proptosis, ocular motility loss, and decreased visual acuity.¹ Histologic studies showed lymphocytic infiltration and edema due to the accumulation of hydrophilic, interstitial glycosaminoglycans in the orbital fat and extraocular muscles,^2,3 attributable to antigenic cross-reactivity between thyroid proteins and orbital fibroblasts.⁴The course of TAO can be divided into the active, regressing, and a burnt-out phase.⁵ Early active disease is treated with combined immunosuppression.^6,7 The identification of patients who stand to benefit from this therapy is important but clinically difficult, despite the availability of activity scoring systems. Consequently, objective methods are needed.CT has been used to quantify the volume of extraocular muscles and orbital fat in TAO.^8–11 However, ocular radiation exposure limits its clinical use. Because MR imaging without ionizing radiation yields orbital images with excellent soft-tissue contrast in any plane, it has been used for evaluating TAO.^12–15The quantitative evaluation of the orbital fat volume on T1-weighted images was reported useful for assessing the severity of TAO and for monitoring the treatment response.^12,16 Recently, Higashiyama et al¹⁷ demonstrated that after methylprednisolone pulse therapy (MPPT), the orbital fat volume was unchanged, while the total volume of extraocular muscles was decreased on T2-weighted images. However, in our search of the literature, we found no reports on quantitative changes in orbital fat edema after MPPT or orbital irradiation. Consequently, the correlation between the volume and edema of orbital fat and the treatment response of patients with TAO remains to be elucidated.FSE iterative decomposition of water and fat with echo asymmetry and least-squares estimation (FSE-IDEAL), a novel 3-point Dixon method, is useful for separating the fat signal from the water signal^18,19 but has not been used to quantify the orbital structures in TAO. To evaluate the feasibility of FSE-IDEAL imaging for the precise quantitative evaluation of orbital fat, we subjected healthy volunteers to 2 FSE-IDEAL imaging studies performed during 1 week. We measured the volume and the water fraction of their orbital fat and ascertained the reproducibility of the measurement results. We also acquired FSE-IDEAL images in patients with TAO to assess MPPT-induced changes in the volume and water fraction of their orbital fat and evaluated the correlation between the quantitative values of orbital fat and the improvement in their symptoms.     

Regional regulation of Filiform tongue papillae development by Ikkα/Irf6

Results : We found Ikkα and Irf6 expression in developing tongue epithelium, and describe here specific tongue abnormalities in mice with mutation of these genes, indicating a role for Ikkα and Irf6 in filiform papillae development. Ikkα and Irf6 mutant tongues showed ectopic vertical epithelium at the midline, while lateral sides of mutant tongues adhered to the oral mucosa. Both the ectopic median vertical epithelium and adhered epithelium exhibited the presence of filiform tongue papillae, whereas epithelium between the median vertical epithelium and adhered tongue showed a loss of filiform tongue papillae. Timing of filiform papillae development was found to be slightly different between the midline and lateral regions of the wild‐type tongue. 相似文献   

Tumor endothelial cells express high pentraxin 3 levels

It has been described that tumor progression has many similarities to inflammation and wound healing in terms of the signaling processes involved. Among biological responses, angiogenesis, which is necessary for tumor progression and metastasis, is a common hallmark; therefore, tumor blood vessels have been considered as important therapeutic targets in anticancer therapy. We focused on pentraxin 3 (PTX3), which is a marker of cancer‐related inflammation, but we found no reports on its expression and function in tumor blood vessels. Here we showed that PTX3 is expressed in mouse and human tumor blood vessels based on immunohistochemical analysis. We found that PTX3 is upregulated in primary mouse and human tumor endothelial cells compared to normal endothelial cells. We also showed that PTX3 plays an important role in the proliferation of the tumor endothelial cells. These results suggest that PTX3 is an important target for antiangiogenic therapy.     

Interventricular dyssynchrony during continuous-flow left ventricular assist device support: observation using the conductance method

Journal of Artificial Organs - The purpose of this study was to observe and clarify the interventricular dysscynchrony caused by continuous-flow left ventricular assist device (CF-LVAD) support...