手助腹腔镜肝血管瘤切除3例

目的探讨手助腹腔镜下切除位置深在或巨大肝血管瘤的安全性及可行性。方法2012年5—12月,实施3例手助腹腔镜肝血管瘤切除术。其中2例S6段肝血管瘤位置深在,1例为S7段10．7cm×6．7cm×7．0cm巨大肝血管瘤。术者直接将左手伸入腹腔,辅助完成腹腔镜肝血管瘤切除术。结果3例均顺利完成手术。手术时间分别为150、230和220min。出血量分别为200、350和500ml。无中转开腹手术。术后住院时间分别为6、5和8d。无术后出血及感染等并发症。结论手助腹腔镜下切除位置深在或巨大肝血管瘤是安全可行的。     

急诊心理护理对急诊患者护理效果的影响分析

目的:评析急诊患者应用心理护理对于提升护理效果的作用.方法:选取我院2019年2月至2020年2月接诊的急诊患者98例,使用抽签法分为对照组(方法:常规护理)和观察组(方法:急诊心理护理),每组各49例,对比两组急诊患者的整体护理效果.结果:观察组收缩压(125.36±11.52)mmHg、舒张压(82.54±4.64...     

Body mass index and migraine: a survey of the Chinese adult population

The objective of this study is to evaluate the association between body mass index (BMI) and migraine in general population Chinese cohort. This was a cross-sectional secondary analysis from a general population Chinese cohort of men and women of reproductive and post-reproductive age ranging between 18 and 65 years. Migraine was defined utilizing ICHD criteria. Body mass indices were calculated using measured height and weight and categorized based on the World Health Organizations criteria. The 1-year period prevalence of migraine was 9.3 %. No association was identified between migraine and those with a BMI < 30.0. Compared to those with normal BMI (18.5–23.0), those with BMI ≥ 30 (morbid obesity) had a greater prevalence of migraine (8.6 vs. 13.8 %, p = 0.000). Multivariate-adjusted odds ratio demonstrated that those with morbid obesity had a greater than twofold increased odds of migraine [OR 2.10 (1.39–3.12)] as compared to those with a BMI between 18.5 and 23.0. No association was found between obesity and migraine severity, frequency, or disability. Morbid obesity was associated with twofold increased odds of migraine in this Chinese men and women cohort of predominantly reproductive age.     

虚拟模拟器在输尿管镜培训中应用的初步探讨

目的 评估虚拟模拟器UroMentorTM在输尿管镜培训中的应用价值. 方法 30名泌尿外科医师按单独完成输尿管镜例数＜ 20例和≥20例分成2组,分别为18名及12名.应用左输尿管下段取石模拟操作进行初评,包括:总操作时间、输尿管插管时间、损伤致出血点的数目、尝试插管次数以及综合总体评分(GRS),连续训练48 h后复评并与初评比较. 结果 经过模拟器训练后所有受试者总操作时间明显缩短[( 333±32)s及(228±18)s,P=0.001],GRS评分明显改善(24.4±2.1及28.1±1.2,P=0.010).少(无)经验组和有经验组初评总操作时间[(405±40)s及(262±22)s,P=0.014]、复评总操作时间[(276±12)s及(179±9)s,P=0.000]及初评GRS评分(19.6±2.5及29.2±1.3,P＝0.009)、复评GRS评分(25.0±1.1及31.2±0.7,P=0.002)差异均有统计学意义.独立完成的输尿管镜例数与GRS评分相关(初评r=0.705,复评r=0.756). 结论虚拟模拟器URO MentorTM可以成为输尿管镜技能培训及评估的有效工具.     

水杨梅化学成分的研究(Ⅲ)

从水杨梅根Adinarubella中分得7个成分，经光谱和化学方法鉴定为：5-hydroxy-2-methylchromone7-O-β-D-xylopyranosyl（1→6）-β-D-glucopyranoside（Ⅰ），5-hydroxy-2-methylchromone7-O-β-D-apiofuranosyl（1→6）-β-D-glucopyranoside（Ⅱ），2，4，6-trimethoxy-Phynol-1-O-β-D-apiofuranosyl-（1→6）-β-D-glucopyranoside（Ⅲ），strictosidinicacid（Ⅳ），barman-3-carboxylicacid（Ⅴ），马钱素（loganin，Ⅵ），2-O-α-D-glucopyranosyl-D-glucose（Ⅶ），其中Ⅲ为新化合物。     

微孔反应板培养法在阴道毛滴虫实验诊断中的应用

近年,笔者对培养法进行了一些改进,以微孔反应板代替培养皿,并对培养液成分进行改进,效果比较理想。1　对象、材料及方法1.1　对象全部为妇科毛滴虫感染疑似病例,患者有白带增多,呈白色泡沫状,稀薄有臭味,外阴瘙痒与腰痛,部分患者有尿痛、尿急、尿频等尿路感染症状。1.2　培养液配制称取NaCl1.0g、NaHCO31.0g、蛋白胨20g、酵母浸膏10g、L-抗坏血酸1.0g、KH2PO410g、K2HPO40.5g、FeSO40.5g、麦芽糖5.0g、半胱氨酸0.1g、氯霉素0.25g、两性霉素B0.01g,蒸馏水加至1000ml,加热至56℃溶解,冷却后加入100ml灭活牛血清或灭活混合人血清,混匀…     

Disturbances in hepatic cell-cycle regulation in mice with assembly-deficient keratins 8/18.

Simple epithelial tissues such as liver and pancreas express keratins 8 (K8) and 18 (K18) as their major intermediate filament proteins. K8 and K18 null mice and transgenic mice that express mutant K18 (K18C) manifest several hepatocyte abnormalities and demonstrate that K8/18 are important in maintaining liver tissue and cell integrity, although other potential functions remain uncharacterized. Here, we report an additional abnormal liver phenotype, which is similar in K8 null, K18 null, and K18C mouse models. Liver histologic examination showed large polynuclear areas that lacked cell membranes, desmosomal structures, and filamentous actin. Similar, but less prominent, areas were observed in the pancreas. The parenchyma outside the polynuclear areas displayed irregular sinusoidal structures and markedly enlarged nuclei. Most K8 null hepatocytes were positive for the proliferating cell nuclear antigen (PCNA) with a doubled DNA content in comparison with the predominantly PCNA-negative wild-type hepatocytes. The distribution of the 14-3-3zeta protein was also altered in K8 null mice. Taken together, our results indicate that absence of keratin filaments causes disturbances in cell-cycle regulation, driving cells into the S-G2 phase and causing aberrant cytokinesis. These effects could stem from disturbed functions of K8/18-dependent cell-cycle regulators, such as the signaling integrator, 14-3-3.     

锤造全冠的适合性实验研究（三种缩颈方法的比较）

用实验方法比较三种常用的缩颈方法及评价锤造全冠的边缘适合性。结果表明各组边缘间隙均大于０．１８０ｍｍ，说明锤造全冠边缘适合性较差，临床检查难以确定其间隙大小。各组近胎面轴壁间隙较小，这与冠成形时此处受力较大，冠周径变小有关，提示临氏上应避免用力和反复锤击此处。各组胎面间隙均大于０．１３０ｍｍ，表明面升高，边缘浮出量较大，冠尚未完全就位。当硬铅代型与缩颈器之间未预留冠套间隙时，所得的锤造全冠适合性更差，面间隙最大（０．９４５ｍｍ）。     

Serological prevalence and risk factor analysis of hepatitis G virus infection in Hubei Province of China

Hepatitis G virus (HGV),also known as GB virus C, is a recently cloned virus which may be associated with human non A-E hepatitis[1,2] It is parenterally transmitted and usually coinfected or superinfected with hepatitis B or hepatitis C virus[3-5]. Some investigations have been reported on the seroprevalence and molecular prevalence of HGV infection in different areas and different population[6-15]. Current infection of HGV is diagnosed by detection of HGV RNA, and past infection with HGV is detectable by testing anti-HGV envelope protein (E2)[16-17]. To investigate the prevalence of HGV in Hubei Province, a central area of the People's Republic of China, ELISA and RT-PCR were employed to detect serum anti-HGV and HGV RNA in 1516 patients who were divided into 16 groups.     

北京地区育龄夫妇不育症的流行病学研究

目的 了解北京地区育龄夫妇不育症患病率及其危险因素.方法 2007年10月至2008年5月,在北京地区6个市辖区和1个远郊县中整群抽取女方年龄为20～49岁的已婚育龄夫妇,设计统一的生育状况调查表,进行不育症患病率现况调查,不育症诊断标准是夫妇双方均有肯定的生育愿望,正常同居2年、有规律性生活、未避孕未孕.描述该人群不育症状况的年龄、城乡分布,比较不育症危险因素暴露的组间差异,进行多因素Logistic回归分析.结果 共调查已婚育龄夫妇5862对,实际调查5632对,调查总体应答率96.07%.共发现不育症夫妇97对,样本的不育症患病率为1.72%,标化率为2.1%,其中原发不育占58.76%;继发不育占41.24%.按女方年龄分组后不育症患病率显示,20～30岁组为3.13%,30～40岁组为1. 69%,40岁以后组为1.38%,不育症患病率随妇女年龄增加而降低(x2=590.08,P＜0.01).按结婚年龄分组,不育妇女有83.5%在20～30岁结婚,其余16.5%在30以后或20岁之前结婚.单因素分析结果提示:影响不育的因素有结核病、子宫内膜异位症和生殖道感染性疾病.多因素Logistic回归分析结果表明,对不育的影响程度从大到小顺序为子宫内膜异位症、结核病、生殖道感染、子宫肌瘤和放射性物质接触史,相对危险度分别为3.13、1.57、4.81、2.62和1.48.其中,子宫内膜异位和生殖道感染史对不育的影响差异有统计学意义(P＜0.05).结论 研究结果反映了北京地区目前生育状况下不育症的总体患病率;不育症患病率随着妇女年龄增高呈下降趋势,结婚年龄偏大在30岁以上妇女不育症的患病率明显增高;子宫内膜异位和生殖道感染对不育的影响显著.

Abstract：

Objective To investigate the prevalence of infertility and its risk factors in the fertileage couples of Beijing, China. Methods A cross-sectional survey on the prevalence and risk factors was conducted from July 2007 to May 2008 in 7 Beijing districts. The districts were subdivided into the administrative units of "communities". A total of 33 communities were sampled and households selected randomly within each community. Of 5862 couples (women aged 20 -49 years) who were eligible for an interview, 5632 (96. 07% ) provided information in relation to all the questionnaire questions. The other couples did not participate because they were not at home for the interview, or became incapacitated, refused or others. The trained interviewers administered a detailed questionnaire to each study couple at their home,collecting the relevant information about contraception, sexual practices, marriage and a complete pregnancy history. The definition of infertility in the study was "a noncontracepting couple who want another child or a child if childless tried to conceive for at least 2 years" following the WHO recommendation[c2]. The study protocol was approved by the ethics committee of National Research Institute for Family Planning (NRIFP).Results A total of 97 couples were classified as infertile. And the total prevalence of infertility in 7 Beijing districts was 1.72%. The standardized prevalence of infertility was 2. 1%. 57 (58. 76% ) couples were classified as of primary infertility and 40 (41.24%) of secondary infertility. So the prevalence of primary and secondary infertilities were 1.01% and 0. 71% respectively. In women, the prevalence of infertility was the highest in the age group of 20 -29 (3.13%) and the lowest in the age group of 40 -49 (1.38%). The prevalence rate of infertility in the women whose marriage age were younger than 20 years or elder than 29 years of age was apparently higher than those of the 20 - 29 years old. The risk factors of infertility were tuberculosis ( TB), endometriosis (EM) and pelvic infection disease (PID). Conclusion The prevalence rate of infertility declines with age in women. Both EM and PID are the risk factors for infertility.