全文获取类型
收费全文 | 1164篇 |
免费 | 84篇 |
国内免费 | 11篇 |
专业分类
耳鼻咽喉 | 14篇 |
儿科学 | 16篇 |
妇产科学 | 8篇 |
基础医学 | 169篇 |
口腔科学 | 46篇 |
临床医学 | 92篇 |
内科学 | 360篇 |
皮肤病学 | 17篇 |
神经病学 | 63篇 |
特种医学 | 52篇 |
外科学 | 182篇 |
综合类 | 8篇 |
预防医学 | 33篇 |
眼科学 | 2篇 |
药学 | 60篇 |
中国医学 | 1篇 |
肿瘤学 | 136篇 |
出版年
2024年 | 2篇 |
2023年 | 19篇 |
2022年 | 41篇 |
2021年 | 99篇 |
2020年 | 51篇 |
2019年 | 40篇 |
2018年 | 52篇 |
2017年 | 38篇 |
2016年 | 40篇 |
2015年 | 38篇 |
2014年 | 57篇 |
2013年 | 48篇 |
2012年 | 106篇 |
2011年 | 106篇 |
2010年 | 51篇 |
2009年 | 47篇 |
2008年 | 63篇 |
2007年 | 54篇 |
2006年 | 51篇 |
2005年 | 49篇 |
2004年 | 34篇 |
2003年 | 53篇 |
2002年 | 41篇 |
2001年 | 6篇 |
2000年 | 2篇 |
1999年 | 5篇 |
1998年 | 7篇 |
1997年 | 6篇 |
1996年 | 8篇 |
1995年 | 4篇 |
1994年 | 8篇 |
1993年 | 5篇 |
1992年 | 5篇 |
1991年 | 4篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1986年 | 3篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1975年 | 1篇 |
1969年 | 1篇 |
排序方式: 共有1259条查询结果,搜索用时 31 毫秒
31.
Nakatsuka Takuma Nakagawa Hayato Hayata Yuki Wake Taijiro Yamada Tomoharu Nishibatake Kinoshita Mizuki Nakagomi Ryo Sato Masaya Minami Tatsuya Uchino Koji Enooku Kenichiro Kudo Yotaro Tanaka Yasuo Kishikawa Takahiro Otsuka Motoyuki Tateishi Ryosuke Koike Kazuhiko 《Journal of gastroenterology》2021,56(5):456-469
Journal of Gastroenterology - Liquid biopsies, particularly those involving circulating tumor DNA (ctDNA), are rapidly emerging as a non-invasive alternative to tumor biopsies. However, clinical... 相似文献
32.
Okusaka Takuji Ikeda Kenji Kudo Masatoshi Finn Richard Qin Shukui Han Kwang-Hyub Cheng Ann-Lii Piscaglia Fabio Kobayashi Masahiro Sung Max Chen Minshan Wyrwicz Lucjan Yoon Jung-Hwan Ren Zhenggang Mody Kalgi Dutcus Corina Tamai Toshiyuki Ren Min Hayato Seiichi Kumada Hiromitsu 《Journal of gastroenterology》2021,56(6):570-580
Journal of Gastroenterology - REFLECT was an open-label, phase 3 study comparing the efficacy and safety of lenvatinib versus sorafenib in patients with unresectable hepatocellular carcinoma... 相似文献
33.
Chieko Sakano Makoto Kuroda Tsuyoshi Sekizuka Taisei Ishioka Yukio Morita Akihide Ryo Hiroyuki Tsukagoshi Yuko Kawai Nobuko Inoue Hayato Takada Yumiko Ogaswara Atsuyoshi Nishina Masa-aki Shimoda Kunihisa Kozawa Kazunori Oishi Hirokazu Kimura 《Journal of clinical microbiology》2013,51(1):328-330
Whole-genome sequencing of non-H2S-producing Salmonella enterica serovar Typhimurium and S. enterica serovar Infantis isolates from poultry meat revealed a nonsense mutation in the phsA thiosulfate reductase gene and carriage of a CMY-2 β-lactamase. The lack of production of H2S might lead to the incorrect identification of S. enterica isolates carrying antimicrobial resistance genes. 相似文献
34.
Akane Yu Hayato Niiyama Shinya Kondo Akiko Yamamoto Ryusuke Suzuki 《Journal of biomaterials science. Polymer edition》2013,24(8):1015-1026
We developed a novel wound dressing composed of a hyaluronic acid (HA) and collagen (Col) spongy sheet containing epidermal growth factor (EGF) or basic fibrolast growth factor (bFGF) by freeze-drying method (EGF-wound dressing or bFGF-wound dressing, respectively). A wound dressing without any growth factor was prepared as a control in a similar manner as above (C-wound dressing). Intermolecular cross-linkage between Col molecules was induced by UV irradiation. The release behavior of free HA from the wound dressing was investigated using a C-wound dressing. The weight of C-wound dressing after 1 day, 3, 5, and 7?days of incubation on top of a Col gel sheet at the air–water interface (wound surface model) was 55, 36, 30, and 19% of the original weight, respectively. Most free HA and a part of Col was released from the cross-linked Col network in the wound dressing during incubation, as the original Col content in the wound dressing was 33%. Next, fibroblast proliferation was assessed in conventional culture medium preconditioned by immersion of a piece of C-, EGF-, or bFGF-wound dressing, i.e. C-conditioned medium, EGF-conditioned medium, or bFGF-conditioned medium. Cell proliferation in C-conditioned medium increased to approximately the same level as that in conventional medium. Cell proliferation in EGF- and bFGF-conditioned medium was 1.9 times and 2.6 times greater than that in conventional medium after 7?days of cultivation, respectively. Finally, cytokine production of fibroblasts was assessed in a wound surface model using a fibroblast-incorporating Col gel sheet (cultured dermal substitute [CDS]). CDS was elevated to the air–medium interface, on which each wound dressing was placed and cultured for 7?days. Fibroblasts in CDS covered with EGF-wound dressing released 3.6 times more vascular endothelial growth factor (VEGF) and 4.6 times more hepatocyte growth factor (HGF) when compared with the C-wound dressing. Fibroblasts in CDS covered with bFGF-wound dressing released 10.2 times more VEGF and 6.3 times more HGF when compared with the C-wound dressing. This finding indicates that bFGF-wound dressing can facilitate more effectively the VEGF and FGF production compared with EGF-wound dressing. 相似文献
35.
Hayato Tada Hirofumi Okada Akihiro Nomura Atsushi Nohara Masakazu Yamagishi Masayuki Takamura Masa-aki Kawashiri 《Journal of clinical lipidology》2021,15(2):358-365
BackgroundFamilial hypercholesterolemia (FH) is an autosomal dominant disorder mainly caused by mutations in the low-density lipoprotein (LDL) receptor or associated genes, resulting in elevated serum cholesterol levels and an increased risk of premature atherosclerotic cardiovascular disease (ASCVD).ObjectiveWe aimed to evaluate the prognostic impact of cascade screening for FH.MethodsWe retrospectively investigated the health records of 1050 patients with clinically diagnosed FH, including probands and their relatives who were cascade-screened, who were referred to our institute. We used Cox models that were adjusted for established ASCVD risk factors to assess the association between cascade screening and major adverse cardiac events (MACE). The median period of follow-up evaluating MACE was 12.3 years (interquartile ranges [IQR] = 9.1–17.5 years), and MACE included death associated with ASCVD, or acute coronary syndrome.ResultsDuring the observation period, 113 participants experienced MACE. The mean age of patients identified through cascade screening was 18-years younger than that of the probands (38.7 yr vs. 57.0 yr, P < 0.0001), with a lower proportion of ASCVD risk factors. Interestingly, patients identified through cascade screening under milder lipid-lowering therapies were at reduced risk for MACE (hazard ratio [HR] = 0.67; 95%CI = 0.44 to 0.90; P = 0.0044) when compared with the probands, even after adjusting for those known risk factors, including age, and prior ASCVD.ConclusionsThe identification of patients with FH via cascade screening appeared to result in better prognosis. 相似文献
36.
Hayato Terayama Shuang-Qin Yi Shuichi Hirai Ning Qu Munekazu Naito Naoyuki Hatayama Shinichi Kawata Masahiro Itoh 《Anatomical science international / Japanese Association of Anatomists》2013,88(3):171-174
Although bilateral megaureters are not an infrequent occurrence in the urinary tract, bilateral megaureters associated with bilateral renal pelvis dilatation and a giant urinary bladder appear to be rare. In this paper, a cadaver case of an adult Japanese male with bilateral megaureters is described. In addition to describing and illustrating this case, the anatomy and etiology of these anomalous structures is discussed with a brief review of the literature. 相似文献
37.
38.
Hayato Sasaki Yoshiaki Murakami Kenichiro Uemura Takeshi Sudo Yasushi Hashimoto Naru Kondo Taijiro Sueda 《International surgery》2015,100(6):1084-1088
Postpancreatectomy hemorrhage is a potentially life-threatening complication. We report herein our experience with a 65-year-old man with locally advanced pancreatic adenocarcinoma who underwent pancreatoduodenectomy with lymphadenectomy following neoadjuvant chemoradiotherapy. On postoperative day 45, he developed massive hematemesis. Angiography revealed active bleeding from the common hepatic artery, and transcatheter coil embolization of that vessel was successfully performed. On postoperative day 64, he again developed massive hematemesis. Angiography revealed active bleeding from the proximal superior mesenteric artery. Immediately after coil embolization of that vessel, bypass grafting between the superior mesenteric artery and the right common iliac artery was performed, using a greater saphenous vein graft. The combination of embolization and bypass grafting is an option for treatment of bleeding from the superior mesenteric artery in an emergent situation.Key words: Superior mesenteric artery, Bleeding, Bypass, Pancreatoduodenectomy, Postpancreatectomy hemorrhagePostpancreatectomy hemorrhage (PPH) is a rare but life-threatening complication, often associated with the presence of a pancreatic fistula or intraabdominal abscess.1 The mortality associated with arterial bleeding after pancreatoduodenectomy is reportedly between 14.3% and 30.7%.2–6 With recent advances in interventional radiology techniques, transcatheter arterial embolization (TAE) has become an alternative to surgical treatment.3,5,7,8 However, it may be difficult to treat these patients with interventional radiology techniques alone, given their often unstable condition. In addition, the inappropriate use of TAE for arterial bleeding, especially after pancreatoduodenectomy, can lead to end-organ infarction and subsequent infection. We report herein our experience with a patient who had bleeding from the superior mesenteric artery (SMA) after pancreatoduodenectomy. This patient was successfully treated using SMA coil embolization followed by creation of an SMA-iliac artery bypass using a greater saphenous vein graft. 相似文献
39.
We analyzed the role of methylthioadenosine phosphorylase (MTAP) for chemoselective treatment of T-cell acute lymphoblastic leukemia (T-ALL). MTAP converts methylthioadenosine into adenine which serves as an alternative purine source, if de novo purine biosynthesis is inhibited by antimetabolites (i.e., methotrexate). The idea of the chemoselectivity concept is that tumors with MTAP deletion at chromosome 9p21 are more susceptible to antimetabolites than normal cells without such a deletion. First, we screened 13 T-ALL lines for 9p21 deletions by comparative genomic hybridization. Five cell lines revealed deletions at the short arm of chromosome 9, dim(9p21pter). Further analyses were performed with CEM cells in which the 9p21 deletion was corroborated by fluorescence in situ hybridization. CEM cells were transfected with an MTAP expression vector. A green fluorescent protein (GFP) plasmid was cotransfected, to monitor the transfection efficacy by flow cytometry. The response of MTAP-transfected cells to the antimetabolites methotrexate (MTX), trimetrexate (TMX), and L-alanosine (ALA) was decreased compared to mock control transfectants using growth inhibition assays. The activity of doxorubicin (DOX) which is not involved in DNA biosynthesis was not changed in MTAP transfectants. As the p16(INK4a) tumor suppressor gene resides also at 9p21, we transfected CEM cells with a p16(INK4a) expression vector. These transfectant cells were more resistant to all four drugs indicating that p16(INK4a) did not specifically affect antimetabolites. The chemoselective effect of antimetabolites in MTAP-deleted tumor cells may, however, be compensated by the development of drug resistance. To prove this possibility, we analyzed an MTX-resistant subline, CEM/MTX1500LV, in which the MTX-resistance conferring dihydrofolate reductase (DHFR) gene was amplified. While TMX exhibited considerable cross-resistance in CEM/MTX1500LV cells, ALA did not. Thus, ALA could exhibit chemoselectivity in 9p21/MTAP-deleted cells, even if DHFR amplification occurs. We conclude that ALA may be more suitable than MTX or TMX for MTAP-mediated chemoselective treatment of T-ALL. Pretherapeutical detection of 9p21 and MTAP deletion may be helpful in developing a predictive molecular chemosensitivity test for T-ALL. 相似文献
40.