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排序方式: 共有2926条查询结果,搜索用时 31 毫秒
81.
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Seale AP Itoh T Moriyama S Takahashi A Kawauchi H Sakamoto T Fujimoto M Riley LG Hirano T Grau EG 《General and comparative endocrinology》2002,125(3):328-339
In the tilapia (Oreochromis mossambicus), as in many teleosts, prolactin (PRL) plays a major role in osmoregulation in freshwater. Recently, PRL-releasing peptides (PrRPs) have been characterized in mammals. Independently, a novel C-terminal RF (arginine-phenylalanine) amide peptide (Carrasius RF amide; C-RFa), which is structurally related to mammalian PrRPs, has been isolated from the brain of the Japanese crucian carp. The putative PrRP was purified from an acid extract of tilapia brain by affinity chromatography with antibody against synthetic C-RFa and HPLC on a reverse-phase ODS-120 column. The tilapia PrRP cDNA was subsequently cloned by polymerase chain reaction. The cDNA consists of 619 bp encoding a preprohormone of 117 amino acids. Sequence comparison of the isolated peptide and the preprohormone revealed that tilapia PrRP contains 20 amino acids and is identical to C-RFa. Incubation of the tilapia pituitary with synthetic C-RFa (100 nM) significantly stimulated the release of two forms of tilapia PRL (PRL188 and PRL177). However, the effect of C-RFa was less pronounced than the marked increase in PRL release in response to hyposmotic medium. The ability of C-RFa to stimulate PRL release appears to be specific, since C-RFa failed to stimulate growth hormone release from the pituitary in organ culture. In contrast, rat and human PrRPs had no effect on PRL release. C-RFa was equipotent with chicken GnRH in stimulating PRL release in the pituitary preincubated with estradiol 17beta. Circulating levels of PRL were significantly increased 1 h after intraperitoneal injection of 0.1 microg/g of C-RFa in female tilapia in freshwater but not in males. These results suggest that C-RFa is physiologically involved in the control of PRL secretion in tilapia. 相似文献
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Kazunori Karasawa Kenichi Asano Shigetaka Moriyama Mikiko Ushiki Misa Monya Mayumi Iida Erika Kuboki Hideo Yagita Keiko Uchida Kosaku Nitta Masato Tanaka 《Journal of the American Society of Nephrology : JASN》2015,26(4):896-906
Monocytes and kidney-resident macrophages are considered to be involved in the pathogenesis of renal ischemia-reperfusion injury (IRI). Several subsets of monocytes and macrophages are localized in the injured tissue, but the pathologic roles of these cells are not fully understood. Here, we show that CD169+ monocytes and macrophages have a critical role in preventing excessive inflammation in IRI by downregulating intercellular adhesion molecule-1 (ICAM-1) expression on vascular endothelial cells. Mice depleted of CD169+ cells showed enhanced endothelial ICAM-1 expression and developed irreversible renal damage associated with infiltration of a large number of neutrophils. The perivascular localization of CD169+ monocytes and macrophages indicated direct interaction with blood vessels, and coculture experiments showed that the direct interaction of CD169+ cell-depleted peripheral blood leukocytes augments the expression levels of ICAM-1 on endothelial cells. Notably, the transfer of Ly6Clo monocytes into CD169+ cell-depleted mice rescued the mice from lethal renal injury and normalized renal ICAM-1 expression levels, indicating that the Ly6Clo subset of CD169+ monocytes has a major role in the regulation of inflammation. Our findings highlight the previously unknown role of CD169+ monocytes and macrophages in the maintenance of vascular homeostasis and provide new approaches to the treatment of renal IRI. 相似文献
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Yukinori Moriyama M.D. Hitoshi Toyohira M.D. Masaaki Koga M.D. Shunichi Watanabe M.D. Hideaki Saigenji M.D. Shinji Shimokawa M.D. Akira Taira M.D. 《The International journal of angiology》1998,7(1):65-67
Coagulopathy is still a frequent complication in the surgical treatment of acute aortic dissection. This study was designed to clarify the influence of acute aortic dissection on the clotting-fibrinolysis system and platelet function. From January 1993 to December 1994 21 patients with proven Stanford type B aortic dissection underwent a series of tests and procedures at our institution. There were 6 women and 15 men, aged 37–74 years (mean 62 years). All patients were admitted within 14 days of onset of dissection. No patient had complications requiring surgery and none died during the observation period. We observed a severe inflammatory reaction with activation of the clotting-fibrinolysis system immediately after onset of dissection. The platelet maximum aggregation rates were also decreased transiently after onset of the dissection. D-dimer values remained elevated throughout, the observation period. A rational approach to the surgical treatment of acute aortic dissection should involve coping with its activated clotting-fibrinolysis system and platelet dysfunction in addition to tissue friability. 相似文献
87.
Kometani K Moriyama M Nakashima Y Katayama Y Wang SF Yamasaki S Saito T Hattori M Minato N 《Blood》2008,112(12):4565-4573
We demonstrate that lck promoter-driven conditional expression of transgenic SPA-1, a Rap GTPase-activation protein, causes a profound defect of alphabeta T-cell development at the CD4/CD8 double-negative (DN) stage due to enhanced cell death without affecting gammadelta T-cell development. The effect was specific to the DN stage, because CD4 promoter-driven SPA-1 expression hardly affected T-cell development. Rap1A17, a dominant-negative Rap mutant, interfered with the generation of double-positive (DP) cells from Rag2(-/-) fetal thymocytes in vitro in the presence of anti-CD3epsilon antibody and Notch ligand. Rap GTPases were activated in a DN cell line by the expression of self-oligomerizing CD3 (CD8:CD3epsilon chimera), which substituted autonomous pre-T-cell receptor (TCR) signal, inducing CD69 expression and CD25 down-regulation. Reciprocally, expression of C3G, a Rap guanine nucleotide exchange factor, in both normal and Rag2(-/-) DN cells markedly enhanced Notch-dependent generation and expansion of DP cells without additional anti-CD3epsilon antibody, thus bypassing pre-TCR. Defective alphabeta T-cell development in the conditional SPA-1-transgenic mice was restored completely by introducing a p53(-/-) mutation. These results suggest that endogenous Rap GTPases downstream of pre-TCR play an essential role in rescuing pre-T cells from the p53-mediated checkpoint response, thus allowing Notch-mediated expansion and differentiation. 相似文献
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Hidetaka Imagita Akira Nishikawa Susumu Sakata Yasue Nishii Akira Minematsu Hideki Moriyama Naohiko Kanemura Hanae Shindo 《Journal of Physical Therapy Science》2015,27(3):791-794
[Purpose] The purpose of this study was to make an experimental model of cervical spinal
cord injury (CSCI) using Wistar rats, in order to analyze the influence of CSCI on the
respiratory function. [Subjects] Thirty-two male 12-week-old Wistar rats were used.
[Methods] The CSCI was made at the levels from C3 to C7, and we performed
pneumotachography and electromyography (EMG) on the diaphragm. Computed tomography was
used to determine the level of spinal cord damage. [Results] After the operation, the
tidal volume of the rats with a C3 level injury decreased to approximately 22.3% of its
pre-injury value. In addition, in the same rats, the diaphragmatic electromyogram activity
decreased remarkably. Compared with before CSCI, the tidal volume decreased to 78.6% of
its pre-injury value in CSCI at the C5 level, and it decreased to 94.1% of its pre-injury
value in CSCI at the C7 level. [Conclusion] In the rats that sustained a CSCI in this
study, the group of respiratory muscles that receive innervation from the thoracic spinal
cord was paralyzed. Therefore, the EMG signal of the diaphragm increased. These results
demonstrate that there is a relationship between respiratory function and the level of
CSCI.Key words: Cervical spinal cord injury, Respiratory function, Electromyography of diaphragm muscle 相似文献
90.