Kinetics of calcium metabolism in lactation

The calcium accretion rate and the size of the exchangeable calcium pool was determined in rats between the 12th and 17th day of lactation after a single intraperitoneal injection of⁴⁷Ca and⁸⁵Sr. The serum specific activity and the retention of both radioactive isotopes in the whole body and tibia (shaft and ends) was determined. The single compartment kinetic model by Bauer was used for the analysis of data.The results show a decrease in the accretion rate and an increase in the exchangeable calcium pool in lactating rats as compared to unmated animals.

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Zusammenfassung Die Geschwindigkeit der Calciumzunahme und die Größe des austauschbaren Calciumpools wurde bei Ratten zwischen dem 12. und 17. Tag der Lactation nach einer einmaligen intraperitonealen Injektion von⁴⁷Ca und⁸⁵Sr bestimmt. Die spezifische Aktivität beider radioaktiven Isotopen im Serum und ihre Retention im ganzen Körper und in der Tibia (Schaft und Epiphysen) wurden bestimmt. Das kinetische Modell von Bauer für ein Kompartiment wurde für die Datenanalyse verwendet.Die Resultate zeigen eine Verminderung der Zunahmegeschwindigkeit und eine Erhöhung des austauschbaren Calciumpools bei laktierenden Tieren, verglichen mit unbegatteten Ratten.

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Résumé Le taux de dépôt du calcium et l'ampleur du calcium total échangeable sont déterminés chez le rat entre le 12ème et 17ème jour de lactation, après une injection intrapéritonéale unique de⁴⁷Ca et⁸⁵Sr. L'activité spécifique du sérum et la rétention des deux isotopes radioactifs dans l'organisme entier et le tibia (diaphyse et épiphyses) sont déterminées. L'analyse des résultats est réalisée à l'aide du modèle cinétique de Bauer à un compartiment unique. C'est ainsi qu'on observe une diminution du taux de dépôt et une augmentation du calcium total échangeable pendant la lactation.

     

Zur Kenntnis der Netzhautveränderungen bei chronischer Bleivergiftung

Ohne ZusammenfassungNach einem Vortrag, gehalten auf der 41. Tagung der Vereinigung mitteldeutscher Augenärzte in Halle am 1. Dezember 1935.     

Zur Frage der Pneumokokkeninfektionen am Auge

Ohne Zusammenfassung     

Zur Frage der Quellbarkeit des Glaskörpers bei Veränderung seiner aktuellen Reaktion

Ohne Zusammenfassung     

Trinkversuch und Glaukomproblem

Ohne ZusammenfassungDie nachstehende Abhandlung ist auf Veranlassung und unter Leitung von Prof.Seidel angefertigt.     

Interrater and Intrarrater Reliability of the Exeter Dysphagia Assessment Technique Applied to Healthy Elderly Adults

The purpose of this study was to evaluate the inter- and intrarater reliabilities of the Exeter Dysphagia Assessment Technique in a sample of elderly adults. This procedure uses noninvasive methods to record aspects of oral motor efficiency and synchronization of respiration during swallowing with the aid of specially developed equipment. Changes in the direction of nasal air flow, time of lip or tongue/spoon contact, and the time/frequency of swallow sounds are monitored and analyzed. Seventy records were evaluated independently by three trained assessors on three consecutive occasions. Interrater reliability was found to be good to very good for five of the respiratory variables assessed and moderate for the sixth. Interrater agreement was also very good for three of the timed oropharyngeal events assessed and moderate for the fourth. Intrarater reliability was very good for the same five respiratory variables and moderate for the sixth. Intrarater agreement was also very good for three of the timed oropharyngeal events and moderate for the fourth. Repeat evaluations of these records showed that agreement between and within raters concerning the sixth respiratory variable was improved substantially when the charts were examined in an enlarged form that provided improved resolution. We conclude that the majority of variables monitored by the Exeter Dysphagia Assessment Technique can be evaluated very reliably.     

Recurrence-free survival after liver transplantation for small hepatocellular carcinoma

Abstract Recurrence-free survival (RFS) in patients with small hepatocellular carcinoma (HCC) after orthotopic liver transplantation (OLT) was analyzed. From 1988 until 1996, 725 OLTs were performed in 669 patients. In 52 adults, HCC was confirmed histologically. OLT was limited to patients with small (<5 cm) HCC with a maximum number of three nodules. Actuarial survival for these 52 patients at 1 and 5 years is 88% and 71%. RFS was defined as time until death without recurrence, time until follow up with a diagnosis of recurrence, or, in patients without recurrence, time of last follow up. Overall, the 5-year RFS was 60%. Five-year RFS was less for bilobar compared to unilobar tumors (36% vs 70%), less for stage IVa tumors (UICC) compared to stage I-III tumors (17% vs 71%), and less for multiple compared to solitary tumors (54% vs 67%). In conclusion, potential cure may be achieved in more than 50% of all transplanted patients.     

p53 Hot-Spot Mutational Analysis in Advanced Western Gallbladder Carcinoma

p53 tumor suppressor gene, have been focusing on this gene’s immunohistochemical detection. From November 1991 to October 1993, seven patients suffering from gallbladder carcinoma underwent surgical resection. Cancerous and normal liver tissues were obtained immediately after surgery, snap-frozen in liquid nitrogen, and stored at −80°C for immunohistochemistry and DNA isolation. Exons 5, 6, 7, and 8 of the p53 gene were completely sequenced following polymerase chain reaction (PCR) amplification of a 1574-bp fragment. Missense mutations were detected in the cancerous tissues of two patients: one transition each on codons 134 (Phe→Leu) and 146 (Trp→Arg). Immunohistochemical p53 staining was positive in the latter patient only. This is the first report on sequence analysis and mutagenesis of the p53 gene in Caucasian patients with gallbladder cancer. Both mutations were transitions and seem to represent a rather rare event. The possible impact of p53 mutagenesis on gallbladder tumorigenesis requires evaluation in larger studies.