全文获取类型
收费全文 | 1137篇 |
免费 | 48篇 |
专业分类
耳鼻咽喉 | 14篇 |
儿科学 | 15篇 |
妇产科学 | 7篇 |
基础医学 | 109篇 |
口腔科学 | 10篇 |
临床医学 | 102篇 |
内科学 | 318篇 |
皮肤病学 | 14篇 |
神经病学 | 81篇 |
特种医学 | 77篇 |
外科学 | 209篇 |
综合类 | 8篇 |
一般理论 | 1篇 |
预防医学 | 52篇 |
眼科学 | 29篇 |
药学 | 46篇 |
肿瘤学 | 93篇 |
出版年
2022年 | 5篇 |
2021年 | 16篇 |
2020年 | 14篇 |
2019年 | 12篇 |
2018年 | 16篇 |
2017年 | 13篇 |
2016年 | 28篇 |
2015年 | 29篇 |
2014年 | 43篇 |
2013年 | 36篇 |
2012年 | 91篇 |
2011年 | 96篇 |
2010年 | 69篇 |
2009年 | 51篇 |
2008年 | 86篇 |
2007年 | 80篇 |
2006年 | 62篇 |
2005年 | 76篇 |
2004年 | 59篇 |
2003年 | 59篇 |
2002年 | 50篇 |
2001年 | 19篇 |
2000年 | 15篇 |
1999年 | 14篇 |
1998年 | 14篇 |
1997年 | 10篇 |
1996年 | 6篇 |
1995年 | 5篇 |
1994年 | 5篇 |
1993年 | 4篇 |
1992年 | 8篇 |
1991年 | 7篇 |
1990年 | 8篇 |
1989年 | 5篇 |
1988年 | 7篇 |
1987年 | 9篇 |
1986年 | 5篇 |
1985年 | 7篇 |
1984年 | 8篇 |
1983年 | 2篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1979年 | 3篇 |
1978年 | 8篇 |
1971年 | 1篇 |
1970年 | 2篇 |
1955年 | 1篇 |
1949年 | 7篇 |
1944年 | 1篇 |
1943年 | 1篇 |
排序方式: 共有1185条查询结果,搜索用时 31 毫秒
991.
992.
PEX6 is Expressed in Photoreceptor Cilia and Mutated in Deafblindness with Enamel Dysplasia and Microcephaly 下载免费PDF全文
Maha S. Zaki Raoul Heller Michaela Thoenes Gudrun Nürnberg Gabi Stern‐Schneider Peter Nürnberg Srikanth Karnati Daniel Swan Ekram Fateen Kerstin Nagel‐Wolfrum Mostafa I. Mostafa Holger Thiele Uwe Wolfrum Eveline Baumgart‐Vogt Hanno J. Bolz 《Human mutation》2016,37(2):170-174
Deafblindness is part of several genetic disorders. We investigated a consanguineous Egyptian family with two siblings affected by congenital hearing loss and retinal degeneration, initially diagnosed as Usher syndrome type 1. At teenage, severe enamel dysplasia, developmental delay, and microcephaly became apparent. Genome‐wide homozygosity mapping and whole‐exome sequencing detected a homozygous missense mutation, c.1238G>T (p.Gly413Val), affecting a highly conserved residue of peroxisomal biogenesis factor 6, PEX6. Biochemical profiling of the siblings revealed abnormal and borderline plasma phytanic acid concentration, and cerebral imaging revealed white matter disease in both. We show that Pex6 localizes to the apical extensions of secretory ameloblasts and differentiated odontoblasts at early stages of dentin synthesis in mice, and to cilia of retinal photoreceptor cells. We propose PEX6, and possibly other peroxisomal genes, as candidate for the rare cooccurrence of deafblindness and enamel dysplasia. Our study for the first time links peroxisome biogenesis disorders to retinal ciliopathies. 相似文献
993.
Hanno Seebens Nicole Schwartz Peter J. Schupp Bernd Blasius 《Proceedings of the National Academy of Sciences of the United States of America》2016,113(20):5646-5651
The human-mediated translocation of species poses a distinct threat to nature, human health, and economy. Although existing models calculate the invasion probability of any species, frameworks for species-specific forecasts are still missing. Here, we developed a model approach using global ship movements and environmental conditions to simulate the successive global spread of marine alien species that allows predicting the identity of those species likely to arrive next in a given habitat. In a first step, we simulated the historical stepping-stone spreading dynamics of 40 marine alien species and compared predicted and observed alien species ranges. With an accuracy of 77%, the model correctly predicted the presence/absence of an alien species in an ecoregion. Spreading dynamics followed a common pattern with an initial invasion of most suitable habitats worldwide and a subsequent spread into neighboring habitats. In a second step, we used the reported distribution of 97 marine algal species with a known invasion history, and six species causing harmful algal blooms, to determine the ecoregions most likely to be invaded next under climate warming. Cluster analysis revealed that species can be classified according to three characteristic spreading profiles: emerging species, high-risk species, and widespread species. For the North Sea, the model predictions could be confirmed because two of the predicted high-risk species have recently invaded the North Sea. This study highlights that even simple models considering only shipping intensities and habitat matches are able to correctly predict the identity of the next invading marine species.The number of alien species transported by human assistance has increased rapidly during the last decades with serious consequences for native flora and fauna (1–3). These biological invasions are considered to be one of the major drivers of biodiversity changes (4–6). Once an unwanted alien species has naturalized in the new environment, it is nearly impossible to eradicate the species, and thus the mitigation of further introduction is the most efficient way of combating biological invasions (6, 7). However, a targeted monitoring and an efficient adaptive management requires knowledge about spreading dynamics of the next potential invaders and thus about the distribution of species, their invasiveness, and the likelihood of new introductions. Although all of these topics have been analyzed on their own, the potential to predict the spreading of alien species while combining these components remains to be tested.A large amount of recent introductions can be attributed to the intensified global trade and transport as many species were accidentally or deliberately translocated through the exchange of commodities or the movements of transportation means (8, 9). The amount of exchanged commodities and the intensity of global traffic have therefore been found to be a good predictor to model the global spread of alien species (10–12). In most cases, predictions of alien species introductions are difficult to assess as model results could not be validated (i.e., quantitatively assessed using observed data) thoroughly due to the paucity of high-quality distributional data of alien species. Without any model validation, however, it is nearly impossible to assess the quality and the reliability of model predictions, which hampers the application of models for the management of alien species. In recent years, appropriate high-quality data have been made accessible by various online databases, but testing model predictions with these data has still been lacking.Model frameworks to predict the likelihood of new invasions have already been developed (10, 11, 13). However, these were not able to predict the identity of new invaders, but only the likelihood that any new species arrives from a certain source region on Earth. Here, we combined such a model, a slightly modified version of the vector-based model of marine invasion adopted from ref. 10, with datasets about the global distribution of marine alien species, which enabled us to predict the identity of the next species to arrive in a given local habitat. The model is a statistical model that describes how the probability a given species successfully invades a specific location depends on the shipping traffic and the environmental differences (temperature and salinity) between locations.In a first step, to test the accuracy of the model, we used native ranges of 40 marine species from various taxonomic groups, ranging from algae to fish, as initial condition. For each species, we simulated the global spread outside its native range and compared the predicted alien range at each simulation time step with the observed one. This procedure allows the assessment of the quality of model predictions, although the degree of expansion distinctly varied among species, as some species are already widespread, whereas others occupy only a few alien regions either because they just started to spread or there are only a limited number of suitable habitats available.In a second step, we used the reported distribution of 97 marine algal species with a known invasion history and six harmful algal species obtained from AlgaeBase (www.algaebase.org) and determined the species-specific invasion probabilities for each marine ecoregion not occupied by that species. Algae are particularly well suited for such an analysis because they are easily translocated by the exchange of ballast water, and especially invasive seaweeds are of global concerns because over 400 introductions have been reported worldwide (14). Furthermore, seaweeds deeply shape marine ecosystems and they can have strong detrimental ecological and economic impacts (14, 15). Using our model, we identified the likely hot spots of future invasions among 90 marine ecoregions of the world and algal species with the highest probability to arrive next. 相似文献
994.
The vitamin A metabolite retinoic acid (RA) plays a fundamental role in cellular functions by activating nuclear receptors. Retinaldehyde dehydrogenase-II (RALDH2) creates localized RA gradients needed for proper embryonic development, but very little is known regarding its regulated expression in adults. Using a human ex vivo model of allergic inflammation by coincubating IgE receptor-activated mast cells (MCs) with blood basophils, we observed prominent induction of a protein that was identified as RALDH2 by mass spectroscopy. RALDH2 was selectively induced in basophils by MC-derived interleukin-3 (IL-3) involving PI3-kinase and NF-kappaB pathways. Importantly, neither constitutive nor inducible RALDH2 expression was detectable in any other human myeloid or lymphoid leukocyte, including dendritic cells. RA generated by RALDH2 in basophils modulates IL-3-induced gene expression in an autocrine manner, providing positive (CD25) as well as negative (granzyme B) regulation. It also acts in a paracrine fashion on T-helper cells promoting the expression of CD38 and alpha4/beta7 integrins. Furthermore, RA derived from IL-3-activated basophils provides a novel mechanism of Th2 polarization. Thus, RA must be viewed as a tightly controlled basophil-derived mediator with a high potential for regulating diverse functions of immune and resident cells in allergic diseases and other Th2-type immune responses. 相似文献
995.
996.
Gaskin DJ Arbelaez JJ Brown JR Petras H Wagner FA Cooper LA 《Journal of the National Medical Association》2007,99(1):22-30
Little is known about why minorities have a lower propensity to use private doctors' offices for their usual source of care than non-Hispanic whites. This study used the 2001 Commonwealth Fund's Health Care Quality Survey of adults to determine if this disparity is due to racial and ethnic differences in attitudes about health and healthcare, and perceptions of racial and ethnic discrimination in healthcare. We found that race and ethnic disparities at the site of the usual source of care persisted even after controlling for individuals' attitudes about health and healthcare, and their perceptions about racial and ethnic discrimination in healthcare. We found that the impact of attitudes and perceptions did vary by subgroups. These factors were important for Asians' site of usual source of care but had little impact on African Americans' site of usual of care. However, despite their differential impact by race and ethnicity, attitudes and perceptions were not the source of observed disparities in site of care. Therefore, in addition to focusing on provider-patient relationships, perhaps future research and policymakers should focus on system-level factors to explain and increase minority use of care in private physicians' offices. 相似文献
997.
998.
999.
1000.