The remedial effect of soluble interleukin-1 receptor type Ⅱ on endometriosis in the nude mouse model

Objective： Recent studies have shown that the local expression of soluble interleukin （IL） -1 receptor type Ⅱ （slL-1 R Ⅱ ） in endometrial tissue of women with endometriosis is decreased, and the depression of IL-1 R Ⅱ was more significant in infertile women than that in fertile women with endometriosis. In this research, we investigated the remedial effect of slL-1-R Ⅱ administration on endometriosis in the nude mouse model. Methods： Nineteen nude model mice with endometriosis were randomly divided into three groups： group A was treated by intraperitoneal administration with only slL-1 R Ⅱ for two weeks, group B was similarly treated with only IL- 1, and group C （control） was administered saline. After 2 weeks, the size of the ectopic endometrial lesions was calculated, and the expression of vascular endothelial growth factor （VEGF） and B-cell lymphoma leukemia-2 （Bcl- 2） were detected by immunohistochemistry. The IL-8 and VEGF levels in the peritoneal fluid （PF） and serum were also measured by enzyme-linked immunosorbent assay （ELISA）. Results： The mean size of ectopic endometrial lesion did not differ between the three groups （P 〉 0.05）. Compared with the control, the expression of VEGF and Bcl-2 was significantly lower in group A, and higher in group B. In the three groups, the levels of IL-8 in the PF and serum were highest in group A, and lowest in group B. Conclusion： slL-1 R Ⅱ may suppresse hyperplasia of ectopic endometriosis, perhaps by reducing the expression of certain cytokines, such as VEGF, IL-8, and Bcl-2, which could provide a new clinical strategy for the treatment of endometriosis.     

改良老年疾病累计评分表在老年消化道肿瘤患者中的应用

目的采用翻译引进的改良老年疾病累计评分表(MCIRS-G)研究老年消化道肿瘤患者的合并症分布情况及其对化疗安全性的影响,评价该量表的临床应用价值。方法80例入院接受治疗的消化道肿瘤患者按照设计好的入组标准进入实验,根据MCIRS-G详细记录患者的合并症情况。年龄≥65岁的患者进入老年组(37例),<65岁者进入对照组(43例),接受以5-Fu为基础的化疗方案治疗,化疗期间及之后四周内纪录出现的各种不良反应。不良反应的评价标准采用NCI3.0版常见不良事件评价标准进行评定,用MCIRS-G分析患者合并症情况,按照MCIRS-G得分、KPS评分和年龄分层研究化疗不良反应发生率的变化。结果两组合并症发病率差异有显著性(P<0.05)。老年组MCIRS-G平均得分16.19±2.25,对照组为14.67±1.08,差异有显著性(P<0.001)。不良反应的影响因素分析显示随着MCIRS-G评分升高老年组白细胞减少(P=0.014)、血小板减少(P=0.010)、贫血(P=0.031)的发生率显著增加,其它不良反应的发生率差异无显著性(P>0.05)。对照组中贫血(P=0.005)的发生率随着MCIRS-G评分升高而显著升高。相关性研究发现MCIRS-G评分与年龄呈正相关(P=0.004),与KPS评分无相关(P=0.375)。结论MCIRS-G在临床上能够有效评价老年消化道肿瘤患者的合并症情况,MCIRS-G评分预测毒副反应比KPS更敏感。年龄不应是老年消化道肿瘤患者接受化疗的限制因素,合并症才是影响化疗安全性的重要因素。     

急性胆道感染时肝细胞线粒体损害的实验研究

为探讨急性胆道感染时肝损害机制,本实验观察了大鼠实验性急性胆道感染对肝细胞线粒体的影响,以及伴随的肝脏储备功能改变。通过对肝细胞线粒体Ca~(2+)含量、溶酶体膜脆性、肝组织过氧化脂质含量、血清OCT,m-GOT酶活性和HPT试验的检测,发现急性胆道感染时肝细胞内存在着线粒体Ca~(2+)超载、溶酶体酶释放、脂质过氧化等,这些均可导致线粒体受累;血清OCT、m-GOT活性升高提示线粒体受损;HPT持续下降,表明线粒体受损同时,肝储备功能进行性受累。     

NLRP3与NOD2受体激活对下游炎症因子表达的影响

目的 核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)与核苷酸结合寡聚化结构域2(NOD2)受体被其特异性配体激活后对下游炎症因子表达的影响.方法 实验分为4组:空白对照组、TATP组、TMDP、TMDP+ATP组.用酶联免疫吸附试验(ELISA)测定白细胞介素(IL)-1β及IL-18的表达,逆转录PCR测定NLRP3、半胱氨酸天冬氨酸蛋白酶1(Caspase-1)的表达情况.结果 TMDP组、TATP组、TMDP+ ATP组的IL-1β和IL-18水平均高于空白对照组(P＜0.05),且TMDP+ ATP组水平增高更显著.TMDP组、TATP组、TMDP+ATP组的NLRP3和Caspase-1表达均高于空白对照组(P＜0.05),且TMDP+ATP2水平增高更显著(P＜0.05).结论 NOD2的特异性配体胞壁酰二肽,NLRP3的特异性配体腺苷三磷酸单独刺激THP-1后NLRP3及下游炎症因子的表达很弱或无表达,联合刺激后可显著增强NLRP3及下游炎症因子的表达,二者之间存在相互促进及协同作用.     

聚乙二醇和胰蛋白酶用于猪动脉管道去细胞效果比较

目的比较0.05%胰蛋白酶-EDTA脱细胞方法与聚乙二醇（poly ethylene glycol，PEG）脱细胞方法对组织物理特性的影响和脱细胞程度。方法用聚乙二醇和胰酶-EDTA处理猪大动脉动脉管道。结果PEG组去细胞百分率为95.32%，胰酶-EDTA组去细胞百分率为90.35%，两组比较P〈0.05，PEG组细胞外基质（ECM）结构保存完整；与对照组比较差异无统计学意义（P〉0.05）。结论PEG法处理动脉管道较传统的组织工程去细胞更为有效，优越性明显，细胞外基质保存完整，生物力学特性稳定。     

航海条件下血液不同保存方法对红细胞表达黏附分子的影响

目的：探讨航海过程中血袋摆放方式对红细胞表达黏附分子的影响。方法于出航训练当日,采集4位健康志愿者全血,按常规方式制备红细胞悬液,每份均分成4份,1份作为对照组保存在医院输血科冰箱,采用直立贴放方式,另3份作为实验组分别采用平铺码放、直立贴放、垂挂固定3种方式同置于舰载储血冰箱。训练结束后,立即检测送至实验室检测红细胞黏附分子CD58, CD44, CD47的表达。结果与陆地保存红细胞比较,航海条件下舰载冰箱直立贴放保存的全血中红细胞表达CD44和CD47的水平显著升高,平铺码放保存的红细胞表达CD58的水平显著降低。结论从表达黏附分子角度,航海条件下,红细胞适宜采用直立贴放方式保存于冰箱中。     

培门冬酶在儿童急性淋巴细胞白血病诱导化疗中的应用研究

目的 :探讨儿童急性淋巴细胞白血病诱导化疗中培门冬酶与左旋门冬酰胺酶的毒副作用以及治疗效果。方法 :从本院2009年2月~2014年2月收治的儿童急性淋巴细胞白血病患者中随机选择95例进行研究,随机分为对照组(47例)和观察组(48例),分别给予左旋门冬酰胺酶和培门冬酶治疗。观察两组的毒副反应发生情况以及治疗效果,并进行比较。结果 :两组在治疗总缓解率和毒副反应发生率方面经比较差异无统计学意义;但观察组的住院时间显著短于对照组,用药次数显著少于对照组,经比较差异均有统计学意义。结论 :儿童急性淋巴细胞白血病诱导化疗过程中,利用培门冬酶和左旋门冬酰胺酶进行治疗均能获得一定的治疗效果,并导致一定毒副作用的出现,但培门冬酶治疗在用药次数和住院时间方面存在明显的优势。     

米口袋化学成分的研究

从米口袋（Gueldenstae dtia multiflora Bge.）中分离得到11个化合物,应用光谱学和化学方法鉴定了其中7个化合物的结构,分别为黑麦草内酯、烈香杜鹃素Ⅰ、木栓醇、木栓酮、4-烯-3-酮豆甾烷、A’-Neogammacer-22（29）-en-3β-ol,acetate和β-谷甾醇。7个化合物均从该植物中首次分离得到,其中化合物Ⅰ是该植物中的水溶性成分。     

阿柏西普与曲安奈德治疗非缺血性CRVO继发黄斑水肿的疗效比较

目的 比较玻璃体腔注射阿柏西普与曲安奈德(triamcinolone acetonide,TA)治疗非缺血性视网膜中央静脉阻塞(central retinal vein occlusion,CRVO)继发黄斑水肿的疗效及安全性.方法 采用临床回顾性分析研究,纳入确诊为非缺血性CRVO继发黄斑水肿患者56例56眼,患者被...     

Identification of novel biomarkers differentially expressed between African-American and Caucasian-American prostate cancer patients

Prostate cancer (PCa) incidence and mortality rate vary among racial and ethnic groups with the highest occurrence in African American (AA) men who have mortality rates twice that of Caucasians (CA). In this study, we focused on differential expression of proteins in AA prostate cancer compared to CA using Protein Pathway Array Analysis (PPAA), in order to identify protein biomarkers associated with PCa racial disparity. Fresh frozen prostate samples (n=90) obtained from radical prostatectomy specimens with PCa, including 25 AA tumor, 21 AA benign, 23 CA tumor, 21 CA benign samples were analyzed. A total of 286 proteins and phosphoproteins were assessed using PPAA. By PPAA analysis, 33 proteins were found to be significantly differentially expressed in tumor tissue (n=48, including both CA and AA) in comparison to benign tissue (n=42). We further compared protein expression levels between AA and CA tumor groups and found that 3 proteins were differentially expressed (P<0.05 and q<5%). Aurora was found to be significantly increased in AA tumors, while Cyclin D1 and HNF-3a proteins were downregulated in AA tumors. Predicted risk score was significantly different between AA and CA ethnic groups using logistic regression analysis. In conclusion, we identified Aurora, Cyclin D1 and HNF-3a proteins as being differentially expressed between AA and CA in PCa tissue. Our study suggests that these proteins might be involved in different pathways that lead to aggressive PCa behavior in AA patients, potentially serving as biomarkers for the PCa racial disparity.