A comparison of bond strengths measured using cantilever bending and micro-tensile methods

Objectives

The purpose of this study was to evaluate the cantilever-bending test as a bond-strength measurement method for enamel/composite adhesion.

Materials and methods

The buccal surface of bovine incisors were flattened and subsequently placed with composite Z250 (3M ESPE) incrementally after either self-etching adhesive (SE) or etch-and-rinse adhesive (SB) was applied. Then, they were cut with a low-speed cutter to obtain stick-shape samples. The samples were divided into four groups (n = 30 each) according to the adhesive and test method: SET, SE-B, SB-T and SB-B, where T denotes the microtensile test and B denotes the cantilever-bending test. The failure mode of each sample was examined under the microscope. Those samples with the fracture surface lying entirely or partially in the adhesive layer were considered to have provided successful measurements. The results from the two test methods were compared.

Results and discussion

The cantilever-bending method produced a comparable success rate of bond strength measurement to that of the microtensile method. The bond strengths of SE and SB from the cantilever-bending test were 58% and 40% higher, respectively, than those measured with the microtensile method. The adhesive SE exhibited almost the same bond strength as SB, irrespective of the test method.

Conclusions

Cantilever bending can be used as an alternative method for bond strength measurement. The ratio of bending to tensile strength is around 1.5.     

CSF-1对体外培养人牙囊细胞OPG表达的影响

目的研究集落刺激因子(CSF-1)对体外培养的人牙囊细胞骨保护素(OPG)表达的影响。方法原代培养人牙囊细胞,传代至第3代,制备细胞爬片,进行OPG免疫组化染色;3~6代人牙囊细胞长至80%时,用胰酶消化,离心,重悬,制成单细胞悬液,接种到细胞培养皿,细胞长满至80%时,培养基中加入25ng/ml的CSF-1,孵育0.5、1、3、6h,分别收集上清液,ELISA法检测OPG蛋白分泌量的变化,提取总RNA进行RT-PCR,检测OPG mRNA的表达变化。结果正常人牙囊细胞OPG蛋白表达阳性;25ng/ml的CSF-1可降低人牙囊细胞OPG的表达,共同孵育1h,OPG表达降至最低(P<0.05)。结论牙囊细胞表达OPG,同时在牙齿萌出的特定时期,CSF-1通过下调人牙囊细胞OPG,减弱对破骨细胞形成的抑制作用,促进破骨细胞分化成熟,调节牙齿萌出。     

Prosthodontic procedures for implant reconstruction. 1. Diagnostic procedures

The University of Sydney Dental School and more recently the Implant Centre have been treating patients with the Brånemark Osseointegration Implant System since 1981. Success depends on close cooperation between surgeon and prosthodontist. This paper describes specific prosthodontic treatment planning procedures based on a general knowledge of prosthodontics, and detailed methods for determining the position, length and alignments of the implants for a specific case by the use of radiographic and surgical templates.     

Post-extraction bleeding—An aid to diagnosis? Case report

Haemophilia A, the most common of bleeding disorders is characterzed by bruishing and spontaneous bleeding into the joints but may remain undiagnosed if present in the mild form. A case is discussed where episodes of bruising and joint sweelling as a child were misdiagnosed as rheumatic fever and the bleeding disorder was diagnosed following recurrent episodes of bleeding after extraction of an upper molar tooth.     

The dental health of Indo-Chinese and Australian-born adolescents

Since 1975, more than 100,000 Indo-Chinese refugees from Vietnam, Kampuchea, and Laos have been resettled in Australia. This dental survey was undertaken to compare the dental health of 104 Australian-born and 126 Indo-Chinese adolescents from a State high school within a high migrant area of Melbourne. The mean DMFT score for the Australian-born group was 3.37 teeth, compared with 6.05 for the Vietnamese group, and 3.10 for the Kampuchean-Laotian group. More restorations and extractions were required by the Vietnamese subjects compared with the Australian-born and Kampuchean-Laotian subjects. Almost all subjects had gingivitis, however the proportion of Indo-Chinese subjects with shallow and deep pockets was greater than that of the Australian-born subjects. The periodontal health of the Kampucheans and Laotians was the most severe of the Indo-Chinese groups. These findings indicate that the Indo-Chinese adolescent refugees represent a high risk group for dental problems within the Australian population.     

Dispensing and weighing apparatus for alginate impression material

Constant proportions in mixing of alginate impression material are difficult to achieve, especially in a teaching department, owing to variability in powder measurement with the conventional scoop method. Apparatus to facilitate powder measurement by weighing is described, and its accuracy assessed.     

正畸移动狗乳磨牙后其恒牙胚牙周组织 CSF-1及TGF-β1的表达及意义

目的：研究狗乳牙正畸移动后其恒牙胚才周组织中集落刺激因子(CSF-1)及转化生长因子-β1(TGF-β1)的表达．探讨乳牙移动后其恒牙胚改变的机制。方法：取狗下颌第二乳磨牙正畸移动后14d的组织标本，用免疫组织化学染色技术观察TGF-β1和CSF-1在恒牙胚牙囊、牙周才槽骨组织中的表达。结果：TGF-β1和CSF-1的染色在乳磨牙牙根的远中牙周膜和牙槽骨中以及恒牙胚的远中牙囊和牙槽骨中的表达明显减弱，在乳磨牙牙根的近中牙周膜和牙槽骨中以及恒牙胚的近中牙囊和牙槽骨中的表达明显增强。结论：狗乳牙正畸移动后其恒牙胚牙周组织也发生骨改建。     

模型转化法建立磨牙及其牙周组织的三维有限元模型

目的建立下颌第二恒磨牙及其牙周膜、牙槽骨的分析用模型。方法应用CAE软件3D MAX6.0及CATIA4.0生成磨牙及其牙周组织的三维有限元模型,利用HYPERMESH划分网格,生成可分析的三维有限元实体模型。结果建立了下颌第二恒磨牙及其牙周膜、牙槽骨的三维有限元模型,可以分别进行受力状态下应力、应变的分析。结论该模型可供口腔正畸矫治中磨牙的受力分析应用。