Candida albicans growth on thermal cycled materials for maxillofacial prostheses in vitro

In the present study, the growth of a single isolate of Candida albicans on saliva-, serum-coated or protein free (uncoated), thermocycled (4-70 degrees C for 1 min, respectively; 0, 1000 and 10 000 times) 15 commercial maxillofacial materials was investigated, by monitoring pH changes in growth media. The inhibitory effect of the tissue conditioners on fungal growth was analysed using three parameters viz: (i) delay in the onset of the rapid decline in pH (ii) reduction in the rate of pH change and (iii) the pH minima reached. In the case of control materials (non-thermocycled and uncoated), significant antifungal effect was observed with two products. However, the antifungal effect of the materials was significantly reduced both by thermal cycling (Analysis of covariance [ANOVA]; P < 0.01) and a layer of protein coating (saliva, P < 0.05; serum, P < 0.01). When the interrelation between three parameters of fungal growth and the surface hydrophobicity of the materials were analysed, minimum pH of fungal growth on 10 000-thermocycled materials correlated well with the contact angles of the materials (Student t-test, P < 0.01), suggesting that thermocycling process reduced the unpolymerized components of the materials which showed the antifungal effects, resulted in that the cell growth depends on the surface hydrophobicity of the specimens. These results, taken together, suggest that the ageing of the materials and the biological fluids of the host enhanced the fungal growth on maxillofacial materials.     

五倍子对感染根管内厌氧菌抑制作用的体外研究

目的:观察中药五倍子对感染根管内几种常见优势专性厌氧菌和兼性厌氧菌的抑菌作用。方法:采用试管二倍稀释法,测定五倍子水提取物在体外环境下对厌氧消化链球菌、内氏放线菌、具核梭杆菌、中间普雷沃菌、金黄色葡萄球菌、大肠杆菌的最小抑菌浓度(MIC)和杀菌浓度(MBC),同时以氢氧化钙做对照。结果:五倍子对各种试验菌株均有抑制作用,对不同的菌株,MIC和MBC值不同,而氢氧化钙对内氏放线菌、金黄色葡萄球菌、大肠杆菌在试验浓度范围内无抑制作用。结论:五倍子水提取物抗菌效果较好且广泛,用于根管内封药具有一定的应用前景。     

大锥度牙胶尖与两种镍钛机动根管预备锉的匹配性研究

目的评价大锥度牙胶尖与两种机动镍钛根管预备锉的匹配性。方法选择40只离体后牙根管,根据初尖锉的号码和近远中向根管弯曲度分为2组,分别以ProTaper和ProFile两种机动镍钛根管锉完成根管预备,插入大锥度牙胶尖进行试尖,比较两者的匹配性。结果ProTaper F1、F2、F3与0.06锥度/20、253、0号牙胶尖的匹配率分别为80%、88%、92%,均较同锥度大1号的牙胶尖匹配率高,差异有显著性(P<0.01)。ProFile 0.06锥度/30号和40号与0.04锥度同号牙胶尖的匹配率最高,分别为83%和96%,其次是0.06锥度小1号牙胶尖,而与0.06锥度同号牙胶尖匹配率最低,分别为35%和25%,3者间差异有显著性(P<0.01)。结论大锥度镍钛机动器械预备的根管,与主尖锉同号而锥度减小或锥度相同且小1号的大锥度牙胶尖有良好的匹配性。     

高龄老年患者全牙列固定义齿修复1例

患者,男性,97岁.2002年10月来就诊,因旧义齿咬合无力要求重新修复.此前曾镶过多副可摘义齿,但均效果不佳. 检查:全身健康状况良好,可与医生进行正常的语言交流.     

Platelet-derived growth factor-B gene delivery sustains gingival fibroblast signal transduction

Background and Objective: Platelet‐derived growth factor‐BB is a potent mediator of tooth‐supporting periodontal tissue repair and regeneration. A limitation of the effects of topical platelet‐derived growth factor‐BB application is its short half‐life in vivo. Gene therapy has shown strong promise for the long‐term delivery of platelet‐derived growth factor in both skin ulcer healing and periodontal tissue engineering. However, little is known regarding the extended effects of platelet‐derived growth factor‐B on cell signaling via gene delivery, especially at the level of phosphorylation of intracellular kinases. This study sought to evaluate the effect of gene transfer by Ad‐PDGF‐B on human gingival fibroblasts (HGFs) and the subsequent regulation of genes and cell‐surface proteins associated with cellular signaling. Material and Methods: HGFs from human subjects were treated by adenoviral PDGF‐B, PDGF‐1308 (a dominant negative mutant of PDGF) and recombinant human platelet‐derived growth factor‐BB, and then incubated in serum‐free conditions for various time points and harvested at 1, 6, 12, 24, 48, 72 and 96 h. Exogenous PDGF‐B was measured by RT‐PCR and Western blot. Cell proliferation was evaluated by [methyl‐³H]thymidine incorporation assay. We used proteomic arrays to explore phosphorylation patterns of 23 different intracellular kinases after PDGF‐B gene transfer. The expression of α and β PDGFR and Akt were measured by Western blot analysis. Results: Sustained in vitro expression of PDGF‐B in HGFs by Ad‐PDGF‐B transduction was seen at both the mRNA and protein levels. Compared to rhPDGF‐BB and Ad‐PDGF‐1308, Ad‐PDGF‐B maintained cell growth in serum‐free conditions, with robust increases in DNA synthesis. Gene delivery of PDGF‐B also prolonged downregulation of the growth arrest specific gene (gas) PDGFαR. Of the 23 intracellular kinases that we tested in proteomic arrays, Akt revealed the most notable long‐term cell signaling effect as a result of the over‐expression of Ad‐PDGF‐B, compared with pulse recombinant human platelet‐derived growth factor BB. Prolonged Akt phosphorylation was induced by treatment with Ad‐PDGF‐B, for at least up to 96 h. Conclusion: These findings further demonstrate that gene delivery of PDGF‐B displays sustained signal transduction effects in human gingival fibroblasts that are higher than those conveyed by treatment with recombinant human platelet‐derived growth factor‐BB protein. These data on platelet‐derived growth factor gene delivery contribute to an improved understanding of these pathways that are likely to play a role in the control of clinical outcomes of periodontal regenerative therapy.     

Type II collagen and aggrecan mRNA expressions in rabbit condyle following disc displacement

The aim of this investigation was to study the remodelling of cartilage in the mandibular condyle following disc displacement (DD) of the temporomandibular joint (TMJ). Forty adult Japanese white rabbits were used in this study. The right joints of 28 of the 40 rabbits had their discs surgically displaced. Four of the 28 were killed at 4 days or 1, 2, 4, 6, 8 and 12 weeks after surgery. The messenger RNA (mRNA) expression levels of aggrecan and type II collagen in cartilages were measured using in situ hybridization techniques. Results showed that aggrecan mRNA expression reduced in the first week after DD. The expression began to recover after 4 weeks and reached a normal level after 6 weeks. Type II collagen mRNA expression reduced from 4 weeks and the expression recovered after 8 weeks. This suggests that the chondrocyte reacting to the displacement of the TMJ disc, alters its matrix gene expression patterns and it is may be the cause of the shape changes of TMJ after DD.     

A comparative evaluation of different compensating curves in the lingual and labial techniques using 3D FEM.

Because adults dislike the visibility of orthodontic appliances, the use of the lingual orthodontic technique has increased over time. But few studies compare tooth movement of the lingual technique with that of the labial technique. In this study, human mandibular left teeth were aligned, and a 3-dimensional finite element model was made (consisting of 19382 nodes and 12150 elements). To compare the effect of compensating curves on canine retraction between the lingual and the labial orthodontic techniques, the compensating curve was increased on the.016-in stainless steel labial or lingual archwire, and a 150-g force was applied distally on the canine. The relative direction and the amount of tooth displacement of the finite element model were compared on a schematic displacement graph (magnified 10,000 times), and the compressive stress distributed on the root surface was observed. The pattern of tooth movement (with or without a compensating curve) was different between the labial and the lingual techniques. As the amount of compensating curve increased (0, 2, and 4 mm) in the archwire, the rotation and the distal tipping of the canine was reduced. The antitip and antirotation action of compensating curve on the canine retraction was greater in the labial archwire than in the lingual archwire.     

CSF-1对体外培养人牙囊细胞OPG表达的影响

目的研究集落刺激因子(CSF-1)对体外培养的人牙囊细胞骨保护素(OPG)表达的影响。方法原代培养人牙囊细胞,传代至第3代,制备细胞爬片,进行OPG免疫组化染色;3~6代人牙囊细胞长至80%时,用胰酶消化,离心,重悬,制成单细胞悬液,接种到细胞培养皿,细胞长满至80%时,培养基中加入25ng/ml的CSF-1,孵育0.5、1、3、6h,分别收集上清液,ELISA法检测OPG蛋白分泌量的变化,提取总RNA进行RT-PCR,检测OPG mRNA的表达变化。结果正常人牙囊细胞OPG蛋白表达阳性;25ng/ml的CSF-1可降低人牙囊细胞OPG的表达,共同孵育1h,OPG表达降至最低(P<0.05)。结论牙囊细胞表达OPG,同时在牙齿萌出的特定时期,CSF-1通过下调人牙囊细胞OPG,减弱对破骨细胞形成的抑制作用,促进破骨细胞分化成熟,调节牙齿萌出。