Gallstones are associated with carotid atherosclerosis

Background/Aims: Gallstone disease (GD) and cardiovascular disease (CD) are common diseases worldwide with considerable economical impact and they are strongly associated. Carotid atherosclerosis is an excellent marker of risk for CD like stroke and myocardial infarction. The aim of this study was to assess the association between gallstones and carotid atherosclerosis. Methods: A cross‐sectional study was conducted. We evaluated subjects with ultrasonographical evidence of GD and asymptomatic subjects without such evidence. Anthropometric, clinical and biochemical variables were collected. The Metabolic syndrome was evaluated using adult treatment panel III criteria. Carotid artery intima–media thickness (CIMT) was determined by a standard ultrasound protocol. Insulin‐like growth factor‐1 (IGF‐1) serum levels were measured in all subjects. Results: We studied 191 subjects: 62 subjects with GD (53.2% males) and 129 asymptomatic subjects without GD (65.9% males). Subjects with GD exhibited a higher body mass index, body fat percent, insulin serum levels and CIMT (P<0.05 for all). The prevalence of GD was higher in subjects with a CIMT>0.75 independently of other factors [odds ratio (OR) 2.12, 95% confidence interval (CI) 1.04–4.34; P=0.039], and for every 0.1 mm increase in CIMT the independent probability to be a case of GD increased by a factor of 1.25 (95% CI 1.02–1.53; P=0.027). IGF‐1 levels did not differ among groups. Conclusions: Subjects with GD exhibit greater carotid atherosclerosis, and therefore have a higher risk for stroke and myocardial infarction.     

Love,Trust, and HIV Risk Among Female Sex Workers and Their Intimate Male Partners

Objectives. We examined correlates of love and trust among female sex workers and their noncommercial male partners along the Mexico–US border.Methods. From 2011 to 2012, 322 partners in Tijuana and Ciudad Juárez, Mexico, completed assessments of love and trust. Cross-sectional dyadic regression analyses identified associations of relationship characteristics and HIV risk behaviors with love and trust.Results. Within 161 couples, love and trust scores were moderately high (median 70/95 and 29/40 points, respectively) and correlated with relationship satisfaction. In regression analyses of HIV risk factors, men and women who used methamphetamine reported lower love scores, whereas women who used heroin reported slightly higher love. In an alternate model, men with concurrent sexual partners had lower love scores. For both partners, relationship conflict was associated with lower trust.Conclusions. Love and trust are associated with relationship quality, sexual risk, and drug use patterns that shape intimate partners’ HIV risk. HIV interventions should consider the emotional quality of sex workers’ intimate relationships.While public health interventions continue to be grounded in tenets of risk and disease, there is growing recognition of the importance of emotions such as love and trust in shaping the health behaviors of diverse populations. Unprotected sex allows for transmission of HIV and other sexually transmitted infections (STIs) but often carries emotional meanings of trust and commitment that impede condom negotiation even among individuals with high knowledge and self-efficacy.1–4 Research has consistently found female sex workers’ condom use to be less frequent with their noncommercial male partners than with clients, which has been linked to intimacy, love, and trust.5–7 Similarly, drug-using couples may share syringes and engage in additional known HIV risk behaviors to convey support and emotional closeness.8,9 Although much of this research is qualitative, some quantitative work has confirmed associations between perceptions of intimacy and condom use among sex workers and clients10 and men who have sex with men.11Quantitative researchers have attempted to operationalize emotions into theoretical constructs for empirical research.12 Sternberg’s “triangular” theory posits that love comprises 3 components: intimacy (feelings of emotional closeness between partners), passion (physical attraction and sexual consummation), and commitment (decisions that partners make about maintaining relationships).13,14 Taken together, these components combine to generate different kinds of love.13,15,16Another important aspect of intimate relationships, trust is conceptualized as a belief in the integrity of a partner. Trust may be based on individuals’ assessments of their partners’ benevolence and honesty17 and subjective feelings of security within relationships.18,19 To measure trust quantitatively, Larzelere and Huston developed a dyadic trust scale that correlated with love and relationship quality.17A rich body of ethnographic scholarship has highlighted the importance of companionate relationships and love in diverse global contexts,20–22 but quantification of emotional constructs are largely absent from settings outside the Western middle-class experience. Most quantitative studies of love and trust have focused on privileged populations who do not experience the burden of disease that disproportionately affects socially marginalized couples. Existing studies among US populations have also suggested that women and men value components of these emotions differently,23–25 yet the implications of these potential gender differences for HIV prevention among vulnerable couples remains underexplored.Our study of love and trust among sex workers and their noncommercial partners is situated in 2 Mexican border cities: Tijuana, Baja California, south of San Diego, California (population 1.6 million), and Ciudad Juárez, Chihuahua, adjacent to El Paso, Texas (population 1.3 million). Both cities have areas where sex work and drug use are concentrated,26 lending to the conceptualization of the border as an HIV risk environment27 characterized by poverty, unemployment, violence, and availability of illicit drugs.28 Research has documented substantial overlap in risky sexual and injection drug use behaviors among marginalized female sex workers29 who often turn to sex work as a last option for supporting themselves and their dependents.30Drawing on Connell’s conceptualization of gender as a relational process influenced by structural and sociocultural factors,31 our previous qualitative and ethnographic inquiry into sex workers’ intimate partnerships in these cities proposed that emotions are shaped by the HIV risk environment of the border. Structural conditions bring partners together into relationships that provide critical material and emotional support. In light of limited economic opportunities, couples form tacit agreements regarding women’s engagement in sex work. In the process, women emerge as primary wage earners, alienating men from traditional gender roles as providers and shifting gender dynamics. To cope, partners mostly avoid communicating about sex work or disclosing HIV risk to protect the emotional integrity of the relationship.32We also found that these couples’ relationships range in emotional intensity from friendship and care to transformative experiences of love. Regardless, most couples demarcate their primary relationships from sex work by engaging in unprotected sex. Among couples who inject drugs, sharing syringes together (but not with others) often reinforces trust and care.33 Concerns of mistrust and infidelity also reduced couples’ interest in alternative HIV-prevention technologies such as pre-exposure prophylaxis.34In light of the central role of emotions in our qualitative research with these couples, we attempted to quantify constructs of love and trust and identify associated relationship traits and HIV risk behaviors in our larger cohort. We hypothesized that markers of relationship quality (e.g., higher satisfaction, lower conflict) and unprotected sex within primary relationships would be associated with higher love and trust, whereas extradyadic HIV risk behaviors (e.g., concurrent sexual partners, syringe sharing with others) would be associated with lower love and trust.     

Economic vulnerability,violence, and sexual risk factors for HIV among female sex workers in Tijuana,Mexico

AIDS and Behavior - Economic vulnerability is often reported to underlie involvement in sex work among female sex workers (FSW), but may also create urgency in women’s work, limiting...     

Cutaneous reactions due to diltiazem and cross reactivity with other calcium channel blockers

BackgroundThe spectrum of cutaneous eruptions in association with calcium channel blockers is extensive, varying from exanthemas to severe adverse events. Reactions due to diltiazem occur more frequently than with other calcium channel blockers. Patch testing has been used as confirmatory testing in patients with extensive cutaneous reactions. Cross-reactivity among these drugs have not been established.MaterialWe present 3 patients: 1) A 54-year-old man developed a generalized eythema-multiformelike reaction followed by erythrodermia and exfoliative dermatitis 6-7 days after starting on diltiazem. The drug was stopped and remission was obtained with emollients and systemic corticosteroids and antihistamines within 12 days. 2) A 80-year-old woman experienced a pruritic exanthematous eruption on her trunk which evolved to generalized erythrodermia and superficial desquamation. This reaction appeared 10 days after taking diltiazem, and gradually improved in 10-12 days after discontinuation of this drug.3) A 79-year-old man presented with erythema and pruritus initially on the back, and then affecting thorax, extremities and face. He had started treatment with diltiazem three days before. Diltiazem was stopped and steroid and antihistamine therapy was given. His skin condition improved, but 3 days later the patient received verapamil with worsening of previous situation. He recovered within 7 days.Methods and resultsTwo to six months after the reaction, we carried out epicutaneous tests with calcium channel blockers from different groups. Diltiazem proved positive (at 48 and 96 hours) in the three patients; nifedipine was also positive in patient 2, and verapamil in patient 3. Controlled administration of verapamil was well tolerated in patient 2 after the reaction, and the patient 1 has taken nifedipine without problems.Conclusions1) We report 3 cases of cutaneous reactions due to diltiazem. 2) Epicutaneous tests have been useful for diagnosis. 3) As one of patients had positive patch tests to diltiazem and nifedipine, and other one with diltiazem and verapamil, more studies are needed to demonstrate cross reactions among calcium channel blockers.     

Characterization of Neuroendocrine Tumors of the Pancreas by Real-Time Quantitative Polymerase Chain Reaction. A Methodological Approach

The aim of this study was to assess the suitability of using real-time quantitative PCR (RT-qPCR) to characterize neuroendocrine (NE) tumors of the pancreas. For a series of tumors, we evaluated several genes of interest, and the data were matched with the “classical” immunohistochemical (IHC) features. In 21 cases, we extracted RNA from formalin-fixed paraffin-embedded (FFPE) blocks, and in nine cases, we also extracted RNA from fresh-frozen tissue. The RT-qPCR procedure was performed using two sets of customized arrays. The test using the first set, covering 96 genes of interest, was focused on assessing the feasibility of the procedure, and the results were used to select 18 genes indicative of NE differentiation, clinical behavior, and therapeutic responsiveness for use in the second set of arrays. Threshold cycle (Ct) values were used to calculate the fold-changes in gene expression using the 2-??Ct method. Statistical procedures were used to analyze the results, which were matched with the IHC and follow-up data. Material from fresh-frozen samples performed better in terms of the level of amplification, but acceptable and concordant results were also obtained from FFPE samples. In addition, high concordance was observed between the mRNA and protein expression levels of somatostatin receptor type 2A (R?=?0.52, p?=?0.016). Genes associated with NE differentiation, as well as the gastrin-releasing peptide receptor and O-6-methylguanine-DNA methyltransferase genes, were underexpressed, whereas angiogenesis-associated markers (CDH13 and SLIT2) were overexpressed in tissues with malignant behavior. The RT-qPCR procedure is practical and feasible in economic terms for the characterization of NE tumors of the pancreas and can complement morphological and IHC-based evaluations. Thus, the results of the RT-qPCR procedure might offer an objective basis for therapeutic choices.     

Improvement of bone repair with l-PRF and bovine bone in calvaria of rats. histometric and immunohistochemical study

Clinical Oral Investigations - The effect of leucocyte- and platelet-rich fibrin (L-PRF), associated with DBBM (deproteinized bovine bone mineral; Bio-Oss®) was investigated and compared with...