山莨菪碱对红细胞自氧化的影响

目的　研究山莨菪碱（Ａｎｉ）在红细胞自氧化损伤中的作用。方法　在红细胞悬液中加入不同浓度Ａｎｉ,温育２４ｈ,分别用比色法、硫代巴比妥酸荧光法、荧光偏振法测定红细胞溶血度、红细胞膜脂质过氧化物（ＬＰＯ） 、红细胞膜微粘度。结果　Ａｎｉ１－０,１－５ ｍ ｍｏｌ·Ｌ－１ 可显著抑制红细胞自氧化溶血（ 溶血度分别为：０－５８５ ±０－０２８、０－４３９ ±０－０２４,对照组为０－７９８ ±０－０３５ ,Ｐ＜０－０１）、显著降低红细胞膜ＬＰＯ〔分别为：（０－３５９ ±０－０１７） 、（０－３２３ ±０－０１９）μｍｏｌ·ｇ－１ Ｐｒｏ,对照组为（０－４１８±０－０１５）μｍｏｌ·ｇ－１ Ｐｒｏ, Ｐ＜ ０－０１〕、显著降低红细胞膜微粘度〔分别为：（０－３２８±０－０１７）、（０－２９６ ±０－０１９）Ｐａ·ｓ,对照组为（０－３７４ ±０－０１４） Ｐａ·ｓ〕。结论　Ａｎｉ 抗氧自由基引发的膜脂质过氧化、保护细胞膜,对红细胞自氧化损伤有保护作用     

原发性气管癌的影像学诊断

 目的：提高原发性气管癌的影像学诊断水平。方法：进行8例原发性气管癌的X线、CT、MRI检查与临床病理对照分析。结果：8例影像学显示气管壁广基底菜花状或乳头状肿块影致管腔狭窄,肿块大小2~4.5cm,1例伴纵膈淋巴结肿大转移。病理诊断：气管鳞癌4例,腺样囊性癌４例。结论：MRI多体位进行气管癌检查,在显示气管癌的形态、大小、气管壁浸润、纵膈淋巴结有无转移等信息优于X线和CT,有助于临床治疗方法选择及预后判断。     

心室晚电位阳性的冠心病患者心肌重构特征分析

对心室晚电位阳性的冠心病患者心肌重构特征进行了分析,为预防冠心病患者发生严重的室性心律失常,从基础理论上做了有益的探讨。     

热休克对氧化应激神经元产生NO的影响

目的 揭示脑在氧化应激状态下一氧化氮产生增多你神经细胞的毒性作用和热休克减少ＮＯ的产生对神经的保护作用机制。方法 对培养大鼠脑皮质神经元分别进行缺氧,Ｈ２Ｏ２,热休克＋缺氧和热休克＋Ｈ２Ｏ２处理,检测丙二醛,超氧化物歧化酶（ＳＯＤ）,ＮＯ和乳酸脱氢酶,并分析它们的变化和相互关系。     

增殖细胞核抗原、雌激素受体及表皮生长因子受体在胆囊肿瘤中的表达

用LSAB免疫组化方法对33例胆囊肿瘤作增殖细胞核抗原（PCNA）、雌激素受体（ER）和表皮生长因子受体（EGFR）检测,结果显示,25例胆囊癌组织中20例PCNA阳性（80％）,腺癌与鳞癌阳性率无显著差异。15例ER阳性（60．0％）,与相应病例PCNA表达等级相关（P＜0．01）。7例EFFR阳性（28．0％）,EGFR阳性病例的ER表达多阴性。在8例腺癌组织中,5例局部PCNA有阳性表达,其中4例该处ER或EGFR阳性。以上结果提示ER及EGFR可能影响胆囊癌细胞的增殖;ER与EGFR之间存在相互调控的关系。三指标在部份胆囊腺瘤的阳性表达应引起注意。     

乳糖化和半乳糖基人生长激素的肝靶向性比较研究

为比较乳糖化人生长激素（ｈＧＨ－１）和半乳糖基人生长激素（ｈＧＨ－Ｇａｌ）的肝靶向性,获得较理想的受体介导肝靶向促生长介素（ＳＯＭ）,以提高人生长激素（ｈＧＨ）治疗侏儒症的效果。作者合成了ｈＧＨ－１和ｈＧＨ－Ｇａｌ,并以＾１３１Ｉ或＾１２５Ｉ标记ｈＧＨ－Ｌ,ｈＧＨ－Ｇａｌ和ｈＧＨ进行小鼠体内分布实验和家兔显像实验对比研究,以及＾１３１Ｉ－ｈＧＨ－Ｌ,＾１３１Ｉ－ｈＧＨ－Ｇａｌ鸡显像和家兔体内竞争结     

The influence of Arhgef1 on pulmonary leukocyte function

Resident leukocytes in the lungs of healthy individuals are necessary for the innate and adaptive immune response toward potentially harmful foreign antigens that are inhaled on a constant basis. Under normal circumstances, inflammatory stimuli are effectively eradicated via innate immunity with accompanying resolution of inflammation and repair of the lung tissue. Work in our lab has explored how Arhgef1, an intracellular signaling molecule expressed by hematopoietic cells, participates in immune function with an emphasis on its contribution to pulmonary immunity. This review summarizes our studies on the role of Arhgef1 in regulating pulmonary macrophage function.     

Dissipation of Clodinafop-Propargyl and Its Metabolite in Wheat Field Ecosystem

The dissipation and residues of clodinafop-propargyl and clodinafop in wheat and soil were investigated by high performance liquid chromatography-tandem mass spectrometry. Clodinafop-propargyl rapidly degraded to the acid derivative-clodinafop as major metabolite in plant and soil. The half-lives of clodinafop-propargyl were 2.35–11.20 days in soil and 6.00 days in wheat plant. The half-lives of clodinafop were 7.04–11.22 days in soil and 0.67–1.24 days in wheat plant. The residues of clodinafop-propargyl and clodinafop in wheat grain, wheat straw and soil were below the detection limit.     

Bioactive insulin microparticles produced by supercritical fluid assisted atomization with an enhanced mixer

Supercritical fluid assisted atomization introduced by a hydrodynamic cavitation mixer (SAA–HCM) was used to micronize insulin from aqueous solution without use of any organic solvents. Insulin microparticles produced under different operating conditions including solution type, solution concentration and precipitator temperature presented distinct morphologies such as highly folded, partly deflated, corrugated or smooth hollow spherical shape. Solution concentration had a striking influence on particle size, and insulin microparticles produced from acidic solution had mean diameters increasing from 1.4 μm to 2.7 μm when protein concentration increased from 3 g/L to 50 g/L. HPLC chromatograms showed no degradation of insulin after SAA–HCM processing and FTIR, CD and fluorescence data further confirmed the structural stability. TGA analysis revealed that insulin microparticles remained moderate moisture content compared with raw material. In vivo study showed that insulin processed by SAA–HCM from acidic solution retained identical bioactivity. SAA–HCM is demonstrated to be a very promising process for insulin inhaled formulation development.