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991.
Update on Pneumocystis carinii f. sp. hominis Typing Based on Nucleotide Sequence Variations in Internal Transcribed Spacer Regions of rRNA Genes 总被引:6,自引:0,他引:6
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Chao-Hung Lee Jannik Helweg-Larsen Xing Tang Shaoling Jin Baozheng Li Marilyn S. Bartlett Jang-Jih Lu Bettina Lundgren Jens D. Lundgren Mats Olsson Sebastian B. Lucas Patricia Roux Antonietta Cargnel Chiara Atzori Olga Matos James W. Smith 《Journal of clinical microbiology》1998,36(3):734-741
Pneumocystis carinii f. sp. hominis isolates from 207 clinical specimens from nine countries were typed based on nucleotide sequence variations in the internal transcribed spacer regions I and II (ITS1 and ITS2, respectively) of rRNA genes. The number of ITS1 nucleotides has been revised from the previously reported 157 bp to 161 bp. Likewise, the number of ITS2 nucleotides has been changed from 177 to 192 bp. The number of ITS1 sequence types has increased from 2 to 15, and that of ITS2 has increased from 3 to 14. The 15 ITS1 sequence types are designated types A through O, and the 14 ITS2 types are named types a through n. A total of 59 types of P. carinii f. sp. hominis were found in this study. 相似文献
992.
本实验用家兔全血加精制大肠杆菌内毒素,体外培养提取粗制家兔内生致热原。给大鼠静脉注射复制发热模型,观察了不同温度保存和不同时间保存的EP对其致热活性的影响。结果表明:4℃保存3天,-40℃保存3天,7天,30天和180天的EP与4℃保存1天的EP比较,其发热第一时相发热峰值和1小时体温反应指数均无显著性差异(P<0.05)。发热第二时相△T和第二时相1小时TRI,在4℃保存3天和-40℃保存3天, 相似文献
993.
Histological review and immunohistochemical studies of 8 cases of medullary carcinoma were carried out by using ABC technique. The results showed 8 calcitonin positive cases, 3 Somatostatin positive cases, 7 NSE positive cases, 5 CEA positive cases and 8 keratin positive cases. In addition, histogenesis, histological characteristics and the evaluation of immunohistochemistry in diagnosis of thyroid medullary carcinoma are discussed. 相似文献
994.
995.
Abstract: Polymorphisms in genes encoding transporters associated with antigen processing (TAP) have been associated with heterogeneity of disease progression in HTV-l-infected homosexual men. In our recent AIDS-related studies of cohorts from Rwanda and Zambia, four new polymorphic sites in the TAP2 coding region were detected by single-strand conformation polymorphism (SSCP) and confirmed by bi-directional nucleotide sequencing and restriction enzyme digestion. The first, a substitution of Thr (GCC) for Ala (ACC) at codon position 374 in exon 5, was found in about 13% of Rwandans and Zambians ( n =213). The remaining 3 new polymorphisms were seen in the 7th exon with changes of 458Thr-ACG to ACA, 466Gly-GGG to GGA, and 467Val-GTT to Ile-ATT, respectively. These 3 variants occurred exclusively on the same chromosome and appeared to have arisen together from the 374Thr-bearing allele. Analyses of the relationship between the 374Thr-457Ile segment and the nearby markers in DQB1 and DRB1 suggested the existence of a unique extended haplotype related to these newly identified variants. 相似文献
996.
Mutations of OCTN2, an organic cation/carnitine transporter, lead to deficient cellular carnitine uptake in primary carnitine deficiency 总被引:3,自引:0,他引:3
Tang NL Ganapathy V Wu X Hui J Seth P Yuen PM Wanders RJ Fok TF Hjelm NM 《Human molecular genetics》1999,8(4):655-660
Systemic primary carnitine deficiency (CDSP, OMIM 212140) is an autosomal recessive disease characterized by low serum and intracellular concentrations of carnitine. CDSP may present with acute metabolic derangement simulating Reye's syndrome within the first 2 years of life. After 3 years of age, patients with CDSP may present with cardiomyopathy and muscle weakness. A linkage with D5S436 in 5q was reported in a family. A recently cloned homologue of the organic cation transporter, OCTN2, which has sodium-dependent carnitine uptake properties, was also mapped to the same locus. We screened for mutation in OCTN2 in a confirmed CDSP family. One truncating mutation (Trp132Stop) and one missense mutation (Pro478Leu) of OCTN2 were identified together with two silent polymorphisms. Expression of the mutant cDNAs revealed virtually no uptake activity for both mutations. Our data indicate that mutations in OCTN2 are responsible for CDSP. Identification of the underlying gene in this disease will allow rapid detection of carriers and postnatal diagnosis of affected patients. 相似文献
997.
Aim
External quality assurance (EQA) is an extremely valuable resource for clinical pathologists to maintain high standards, improve diagnostic skills, and possibly revalidate medical license. The aim of this study was to participate in and compare four international slide survey programs (UK, IAP-Germany, USA-Canada, Australasia) in pediatric histopathology for clinical pathologists with the aim to use it as a revalidation method. 相似文献998.
Zuochen Du Anwei Chen Lu Huang Xin Dai Qiuyue Chen Di Yang Liling Li Heather Miller Lisa Westerberg Yuan Ding Xuemei Tang Masato Kubo Liping Jiang Xiaodong Zhao Hua Wang Chaohong Liu 《The Journal of allergy and clinical immunology》2021,147(5):1907-1923.e6
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999.
Michelle K Williamson Jane Pirkis Jon J Pfaff Orla Tyson Moira Sim Ngaire Kerse Nicola T Lautenschlager Nigel P Stocks Osvaldo P Almeida 《BMC medical research methodology》2007,7(1):42
Background
Recruiting and retaining GPs for research can prove difficult, and may result in sub-optimal patient participation where GPs are required to recruit patients. Low participation rates may affect the validity of research. 相似文献1000.
将白细胞介素-1(IL)注入大鼠侧脑室,用Fos癌蛋白抗体免疫组化法检测了下丘脑室旁核的激活神经元:大量位于含促肾上腺皮质激素释放因子(CRF)相应区域的室旁核小细胞部神经元呈Fos免疫强阳性。Fos和CRF的免疫双染色显示了许多Fos免疫阳性神经元也呈CRF免疫阳性。此外,在IL-1注射动物中,CRF的免疫阳性显著加强,提示CRF合成增加。 相似文献