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21.
Subarna Bhattacharyya Punarbasu Chaudhuri Siddartha Dutta Subhas Chandra Santra 《Bulletin of environmental contamination and toxicology》2010,84(5):618-622
Total mercury levels were quantified in Tilapia mossambicus, Cirrhinus mrigela and Labio rohita, captured from East Calcutta Wetlands and Titagarh sewage fed aquaculture ponds. The bioconcentration factor of collected
fish was assessed. Total mercury level ranged from 0.073 to 0.94 μg/g in both pre and post monsoon season. T. mossambicus in both season and C. mrigela at pre monsoon, cross the Indian recommended maximum limit (0.50 μg/g wet weight) for food consumption and according to World
Health Organization guidelines all fish were not recommended for pregnant women and individuals under 15 years ages. A significant
correlation was observed between mercury content of aquaculture pond water and fish muscle tissue. Total mercury concentration
in experimental sites were higher than the control area (Wilcoxon Ranked-Sum test p > 0.05), which suggested the connection between mercury bioaccumulation and sewage fed aquaculture. 相似文献
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Silk fibroin scaffolds with muscle‐like elasticity support in vitro differentiation of human skeletal muscle cells
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Vishal Chaturvedi Deboki Naskar Beverley F. Kinnear Elizabeth Grenik Danielle E. Dye Miranda D. Grounds Subhas C. Kundu Deirdre R. Coombe 《Journal of tissue engineering and regenerative medicine》2017,11(11):3178-3192
Human adult skeletal muscle has a limited ability to regenerate after injury and therapeutic options for volumetric muscle loss are few. Technologies to enhance regeneration of tissues generally rely upon bioscaffolds to mimic aspects of the tissue extracellular matrix (ECM). In the present study, silk fibroins from four Lepidoptera (silkworm) species engineered into three‐dimensional scaffolds were examined for their ability to support the differentiation of primary human skeletal muscle myoblasts. Human skeletal muscle myoblasts (HSMMs) adhered, spread and deposited extensive ECM on all the scaffolds, but immunofluorescence and quantitative polymerase chain reaction analysis of gene expression revealed that myotube formation occurred differently on the various scaffolds. Bombyx mori fibroin scaffolds supported formation of long, well‐aligned myotubes, whereas on Antheraea mylitta fibroin scaffolds the myotubes were thicker and shorter. Myotubes were oriented in two perpendicular layers on Antheraea assamensis scaffolds, and scaffolds of Philosamia/Samia ricini (S. ricini) fibroin poorly supported myotube formation. These differences were not caused by fibroin composition per se, as HSMMs adhered to, proliferated on and formed striated myotubes on all four fibroins presented as two‐dimensional fibroin films. The Young's modulus of A. mylitta and B. mori scaffolds mimicked that of normal skeletal muscle, but A. assamensis and S. ricini scaffolds were more flexible. The present study demonstrates that although myoblasts deposit matrix onto fibroin scaffolds and create a permissive environment for cell proliferation, a scaffold elasticity resembling that of normal muscle is required for optimal myotube length, alignment, and maturation. © 2016 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons Ltd. StartCopTextStartCopText© 2016 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons Ltd. 相似文献
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With the epidemic increase in obesity in the USA and consequent increased demand for bariatric surgery, new complications
of the surgery are being described. The most common surgery practiced is the Roux-en-Y gastric bypass (RYGBP). Unraveling
of suture material at the gastrojejunal anastomosis may occur, which may be troublesome if nonabsorbable suture is employed.
We describe, for the first time, two patients who developed obstructive symptoms as a consequence of food matter/bezoars entrapped
within a mesh of unraveled nonabsorbable suture material at their anastomoses. One of these patients also developed ulceration,
presumably as a result of pressure necrosis from the entrapped bezoar. We describe a third patient where the placement of
nonabsorbable sutures led to obstructive symptoms by limiting distensibility at an otherwise satisfactory anastomosis. We
also describe for the first time, the use of a new endoscopic scissors in cutting luminal suture material with subsequent
resolution of the clinical problem. 相似文献
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Gabriel A Shores J Heinrich C Baqai W Kalina S Sogioka N Gupta S 《International wound journal》2008,5(3):399-413
This data review reports the results of 15 patients who were treated with Vacuum-Assisted Closure (VAC) negative pressure therapy system in addition to the timed, intermittent delivery of an instilled topical solution for management of their complex, infected wounds. Prospective data for 15 patients treated with negative pressure wound therapy (NPWT)-instillation was recorded and analysed. Primary endpoints were compared to a retrospective control group of 15 patients treated with our institution's standard moist wound-care therapy. Culture-specific systemic antibiotics were prescribed as per specific patient need in both groups. All data were checked for normality of distribution and equality of variance and appropriate parametric and non parametric analyses were conducted. Compared with the standard moist wound-care therapy control group, patients in the NPWT-instillation group required fewer days of treatment (36.5 +/- 13.1 versus 9.9 +/- 4.3 days, P < 0.001), cleared of clinical infection earlier (25.9 +/- 6.6 versus 6.0 +/- 1.5 days, P < 0.001), had wounds close earlier (29.6 +/- 6.5 versus 13.2 +/- 6.8 days, P < 0.001) and had fewer in-hospital stay days (39.2 +/- 12.1 versus 14.7 +/- 9.2 days, P < 0.001). In this pilot study, NPWT instillation showed a significant decrease in the mean time to bioburden reduction, wound closure and hospital discharge compared with traditional wet-to-moist wound care. Outcomes from this study analysis suggest that the use of NPWT instillation may reduce cost and decrease inpatient care requirements for these complex, infected wounds. 相似文献
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Roopashri Rajesh Kashyap Gopakumar R. Nair Subhas Babu Gogineni Kumari N. Suchetha 《International journal of diabetes in developing countries.》2011,31(4):194-198
This study was undertaken to determine the effect of smoking and diabetes mellitus individually and combined on the total antioxidant capacity (TAC) of saliva in type 2 diabetes mellitus. The study consisted of four groups, one control and three study groups, each with 20 subjects. Salivary and serum samples were collected from all the groups, which were further subjected to biochemical analysis. The observations of the study were subjected to statistical analysis using SPSS software version 12. The level of salivary TAC decreases secondary to diabetes mellitus and smoking. As the duration of diabetes mellitus increases, the level of total antioxidants decreases. Correlation of salivary TAC with Russell’s index had a negative correlation. Smoking has a synergistic effect on diabetes mellitus in reducing the salivary total antioxidant level. Further research should be devoted to the possible benefits of supplementation with antioxidant supplements. Saliva is considered to be a representative of serum and salivary analysis is simple and noninvasive. From the clinical standpoint, it may be reasonable to conclude that salivary TAC can be a useful marker to assess the oxidative stress, as an indicator of progression of diabetes mellitus. 相似文献
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Jasneet Singh Bhullar Gokulakkrishna Subhas Aditya Gupta Michael J. Jacobs Melissa Decker Boris Silberberg Vijay K. Mittal 《JSLS, Journal of the Society of Laparoendoscopic Surgeons》2012,16(4):606-611