Peripheral blood stem cell donation: an analysis from the International Bone Marrow Transplant Registry (IBMTR) and European Group for Blood and Marrow Transplant (EBMT) databases

Donation-related data for 1488 allogeneic peripheral blood stem cell (PBSC) transplants reported to the International Bone Marrow Transplant Registry (IBMTR) or the European Blood and Marrow Transplant Group (EBMT) by 152 teams worldwide between 1994 and 1998 were reviewed. In 1998, 26% of allografts registered with the IBMTR were collected from blood. Median age of PBSC donors was 38 years (range <1-76), and 55% were male. Of 1486 donor-recipient pairs evaluable for HLA compatibility, 1322 (89%) were HLA-identical siblings. Recombinant human granulocyte colony-stimulating factor (G-CSF) was employed to mobilize PBSCs in almost all (99%) cases. One hundred and seventy (20%) of 828 evaluable PBSC donors had a central catheter placed for leukapheresis. Eighty-five percent of 1321 evaluable PBSC grafts were collected with one or two leukaphereses. There were 15 reported donation-related adverse events (1% of evaluable donors). Complications were catheter-related in five. No donation-related fatalities were reported. These data suggest that PBSC donation is becoming more prevalent worldwide. It appears to have a safety profile comparable to marrow harvesting, although experience with the latter is much more extensive.     

流式细胞术对小鼠外周血红细胞微核自动化检测的研究

本文报道双激光流式术对小鼠外周血嗜多染红细胞（ＰＣＥ）和成熟红细胞（ＮＣＥ）微核（ＭＮ）的自动化检测新方法。用噻唑橙染ＲＮＡ以区分ＰＣＥ和ＮＣＥ，用Ｈｏｅｃｈｓｔ３３３４２染ＤＮＡ以检测红细胞内ＭＮ。在用ＭＭＣ和ＣＯＬ进行的ＭＮ量效和时效关系研究中，其机检们和镜检结果吻合很好（ｒ＞０．９５），流式仪分选验证，证实其ＭＮ真实性在９５％以上，而检测速度比人工提高４０倍以上。本方法具有高速、精确、客观和完全自动化的特点，标志着流式仪对ＭＮ的自动化检测已达到实用化程度。     

Hemodynamic effects of a new inotropic compound,PST-2744, in dogs with chronic ischemic heart failure

Inotropic agents for acute decompensated heart failure are associated with a lack of efficacy or increased mortality. New compounds are needed to support patients with acute exacerbations of heart failure. This study examined the hemodynamic effects of a new inotropic agent (PST-2744) in dogs with chronic ischemic heart failure. Eight mongrel dogs at low risk for postmyocardial infarction (MI) sudden death entered the protocol. Dogs were studied after ischemic left ventricular dysfunction was induced by repeated injections of latex microspheres into the circumflex artery until the ejection fraction reached 35%. Hemodynamic parameters were measured at baseline and peak drug effect (PST-2744 5 microg.kg-1.min-1). In 5 animals, PST-2744 effects were compared with dobutamine. Heart rates, PR intervals and QT intervals were unchanged following PST-2744 administration. PST-2744 increased contractility (+dP/dt) by 56% from 1881 +/- 282 mm Hg/s to 2939 +/- 734 mm Hg/s (P < 0.01). The inotropic effect of PST-2744 was equal to that produced by 5-microg.kg-1.min-1 dobutamine (56% increase in +dP/dt), but peak heart rates were significantly higher with dobutamine (129 +/- 24 bpm PST-2744 versus 160 +/- 6 bpm 5-microg.kg-1.min-1 dobutamine, P < 0.002). No arrhythmias or conduction delays were seen with either compound. PST-2744 is an effective inotropic agent without positive chronotropic effect in subjects with stable moderate left ventricular dysfunction.     

Unrecognized shoulder joint penetration during fixation of proximal fractures of the humerus

In osteosynthesis of proximal humerus fractures, the screws frequently penetrate the joint. Using orthogonal frontal and lateral radiographs, we found unrecognized pin penetration in 8 of 30 patients during fixation of unstable neck fractures of the humerus.     

动情周期和妊娠期豚鼠宫颈张力的变化与性激素水平的关系

本文用离体宫颈张力测定法及放射免疫测定法证实,动情周期中豚鼠宫颈张力的变化与血中孕酮水平呈正相关。妊娠期豚鼠宫颈张力变化明显。孕62天及65天时,宫颈张力极度减弱,宫颈口开大,耻骨联合松弛,宫颈增重,这些生理变化是受血中P、E_2、PGFM及羊水中PGFM浓度的影响。     

灵芝孢子粉碱提多糖对小鼠巨噬细胞的免疫调节作用

目的 :研究灵芝孢子粉碱提多糖 (LZSBS)对小鼠巨噬细胞的激活作用。方法 :用灵芝孢子粉碱提多糖刺激体外培养的小鼠巨噬细胞 ,用ELISA法检测巨噬细胞分泌至培养基中的TNF α和IL 1β的含量 ;用Griess法检测培养上清中NO的含量。小鼠巨噬细胞对乳胶颗粒的吞噬率用显微镜计数。结果 :经灵芝孢子粉碱提多糖刺激后 ,小鼠巨噬细胞变大 ,颜色加深 ,并能显著刺激巨噬细胞分泌TNF α和IL 1β ,并产生大量的NO。小鼠巨噬细胞对乳胶颗粒的吞噬功能也明显的增强。结论 :灵芝孢子粉碱提多糖对小鼠巨噬细胞具有明显的激活作用