党的先进性建设与构建和谐社会

目的加强党的先进性建设,加强党的执政能力建设,同构建社会主义和谐社会紧密相联,加强党的先进性建设,将有力促进构建社会主义和谐社会。方法按照辨证唯物主义的观点,揭示党的先进性与构建和谐社会之间的关系。即:构建社会主义和谐社会,是党的先进性建设的内在要求;党的先进性建设是构建社会主义和谐社会的重要保证。结果在加强党的先进性建设中,必须努力提高构建社会主义和谐社会的能力。结论保持共产党员先进性教育,加强党的先进性建设必须紧紧围绕构建社会主义和谐社会这个重大任务来进行。     

高效液相色谱法测定补骨脂中三种黄酮类成分的含量

目的 :建立补骨脂中三种黄酮类成分含量测定方法。方法 :采用高效液相色谱法分析补骨脂中补骨脂甲素和补骨脂异黄酮的含量。色谱柱 :汉邦 Lichrosphore C1 8(2 5 0 mm× 4.6mm ,5μm) ;流动相 :乙腈 -水 (4∶6) ;流速 1.0 ml/ min;检测波长 :2 79nm(0～ 3 0 min) ,2 47nm (3 0～ 45 min) ;进样量 2 0μl。补骨脂查耳酮 :色谱柱 :汉邦 L ichrosphore C1 8(2 5 0 mm× 4.6mm,5 μm) ;流动相 :甲醇 -水 (85∶ 15 ) ;流速 1.0 ml/ min;检测波长 3 87nm ;进样量 2 0μl。结果 :平均加样回收率为 98.2 % ,RSD为 1.3 % ,0 .0 489～ 0 .3 91μg,r=0 .9999(n=7)。结论 :该方法适合于测定补骨脂中黄酮类成分的含量 ,应用该方法对不同产地的补骨脂中 3种成分含量进行了测定。不同产地中黄酮类成分的含量存在很大差异 ,对补骨脂黄酮类成分进行含量测定应成为补骨脂质量控制的重要环节。     

SFE-CGC法测定腰痛片中主要成分的含量

 目的 建立超临界流体萃取(SFE)技术和毛细管气相色谱(CGC)非在线联用技术,测定中成药腰痛片中的主要成分。方法 以SFE法进行样品前处理,CGC法采用含5%苯基硅氧烷交联毛细管柱,蒽作为内标进行定量。结果 对国内5个厂家生产的腰痛片中的补骨脂素和异补骨脂素进行测定,含量分别为：补骨脂素0.11～0.51 mg·g^-1,异补骨脂素0.21～0.58 mg·g^-1。结论 SFE-CGC法是腰痛片质量控制的快速可靠、准确灵敏的方法。     

Biomedical Support Requirements in the Space Era

Chest radiographs made on 1,007 canine subjects at the University of Pennsylvania Veterinary Hospital between 1965 and 1968 were reviewed to determine whether a relationship between nonspecific chronic canine pulmonary disease and the urban environment could be demonstrated. Radiographs were graded for evidence of chronic pulmonary changes without prior knowledge of the animal’s age or residence. The environment was divided into urban and rural segments based upon the concentration of industrialization in the Philadelphia area and available atmospheric pollution data. No significant differences were noted in the urban-rural distribution of absent, moderate, and severe changes in younger dogs. In middle-aged and older dogs, however, significant differences appeared in the distribution of changes between environmental groups.     

蒙药治疗肝损伤的实验研究进展

随着蒙医药临床与实验科学的快速发展，关于肝损伤的实验研究也逐渐增多，其研究内容从最初的药物对实验动物生化指标的影响发展到组织病理形态学改变以及分子生物，免疫蛋白测定等。本文章通过查阅与总结从1992年至今关于蒙药治疗肝损伤实验研究，总结多种蒙药复方、单药及其提取物对不同模型导致肝损伤的作用及影响，为蒙药治疗肝损伤的进一步研究发展提供最初的信息资源。     

Effects of vasopressin during a pulmonary hypertensive crisis induced by acute hypoxia in a rat model of pulmonary hypertension

Background

A pulmonary hypertensive crisis (PHC) can be a life-threatening condition. We established a PHC model by exposing rats with monocrotaline (MCT)-induced pulmonary hypertension to acute hypoxia, and investigated the effects of vasopressin, phenylephrine, and norepinephrine on the PHC.

Molecular interaction of imino sugars with human α-galactosidase: Insight into the mechanism of complex formation and pharmacological chaperone action in Fabry disease

Enzyme enhancement therapy (EET) for Fabry disease involving imino sugars has been developed and attracted interest. It is thought that imino sugars act as pharmacological chaperones for wild-type and mutant α-galactosidases (GLAs) in cells, but the mechanisms underlying the molecular interactions between the imino sugars and the enzyme have not been clarified yet. We examined various kinds of imino sugars and found that galactostatin bisulfite (GBS) inhibited GLA in vitro and increased the enzyme activity in cultured Fabry fibroblasts as in the case of 1-deoxygalactonojirimycin (DGJ). Then, we analyzed the molecular interactions between the imino sugars and recombinant human GLA by means of isothermal titration calorimetry and surface plasmon resonance biosensor assays, and first determined the thermodynamic and binding-kinetics parameters of imino sugar and GLA complex formation. The results revealed that DGJ bound to the enzyme more strongly than GBS, the binding of DGJ to the enzyme protein being enthalpy-driven. In the case of GBS, the reaction was mainly enthalpy-driven, but there was a possibility that entropy-driven factors were involved in the binding. Structural analysis in silico revealed that both the chemicals fit into the active-site pocket and undergo hydrogen bonding with residues comprising the active-site pocket including the catalytic ones. The side chain of GBS was oriented towards the entrance of the active-site pocket, and thus it could be in contact with residues comprising the wall of the active-site pocket. Thermodynamic, kinetic and structural studies should provide us with a lot of information for improving EET for Fabry disease.