Histologic evaluation of radiofrequency ablation in renal cancer.

AIM: To report on the histologic evaluation of renal tumors after intraoperative radiofrequency ablation (RFA) performed immediately before surgical nephrectomy. MATERIALS AND METHODS: Ten patients with renal tumors were studied. All tumors were confirmed to be renal cell carcinoma before radio frequency ablation treatment. All specimens were stained with hematoxylin and eosin and nicotinamide adenine dinucleotide (NADH). RESULTS: Mean tumor size was 29 mm (range 14 to 48 mm). Of the 10 tumors 5 were completely devitalized with a treatment margin ranged from 1 to 20mm. One tumor needed two treatment sessions. No complication related to radiofrequency treatment was recorded. CONCLUSIONS: Our data indicate that RFA can completely destroy renal tumors. However, in our study, complete tumor cell death was obtained only in 50% of cases. More studies are needed to ensure that is technique is an effective and reproducible treatment.     

FK506-壳聚糖膜与体外培养雪旺细胞生物相容性的研究

目的 探讨FK506-壳聚糖膜片与培养的雪旺细胞生物相容性.方法 将生长状况和纯度较好的第3代雪旺细胞,分别传代接种于含有FK506-壳聚糖膜片、单纯壳聚糖膜片的培养皿中培养,以盖玻片作为对照组.计算3组雪旺细胞倍增时间、雪旺细胞纯度;用MTT法比较不同膜片上雪旺细胞的生长活力,绘制生长曲线;S-100免疫荧光染色.结果 雪旺细胞倍增时间对照组为5.9 d,单纯壳聚糖膜片及FK506-壳聚糖膜片组均为4.0 d;雪旺细胞纯度分别为80%、89%,93%;雪旺细胞增殖情况以FK506-壳聚糖组最佳,单纯壳聚糖组次之,对照组最差;各组雪旺细胞均呈S-100阳性,FK506-壳聚糖组与单纯壳聚糖组雪旺细胞排列呈典型的漩涡状或栅栏状.结论 FK506-壳聚糖膜片具有更显著的组织相容性与生物功能性,并保持了雪旺细胞的生物学特性.     

Tics moderate treatment outcome with sertraline but not cognitive-behavior therapy in pediatric obsessive-compulsive disorder.

BACKGROUND: The presence of a comorbid tic disorder may predict a poorer outcome in the acute treatment of pediatric obsessive-compulsive disorder (OCD). METHODS: Using data from the National Institute of Mental Health (NIMH)-funded Pediatric OCD Treatment Study (POTS) that compared cognitive-behavior therapy (CBT), medical management with sertraline (SER), and the combination of CBT and SER (COMB), to pill placebo (PBO) in children and adolescents with OCD, we asked whether the presence of a comorbid tic disorder influenced symptom reduction on the Children's Yale-Brown Obsessive Compulsive Scale (CY-BOCS) after 12 weeks of treatment. RESULTS: Fifteen percent (17 of 112) of patients exhibited a comorbid tic disorder. In patients without tics, results replicated previously published intent-to-treat outcomes: COMB > CBT > SER > PBO. In patients with a comorbid tic disorder, SER did not differ from PBO, while COMB remained superior to CBT and CBT remained superior to PBO. CONCLUSIONS: In contrast to CBT outcomes, which are not differentially impacted, tic disorders appear to adversely impact the outcome of medication management of pediatric OCD. Children and adolescents with obsessive-compulsive disorder and a comorbid tic disorder should begin treatment with cognitive-behavior therapy alone or the combination of cognitive-behavior therapy plus a serotonin reuptake inhibitor.     

Infected chronic hematoma mimicking soft tissue neoplasm

A rare case of an infected chronic hematoma in a patient with immunodeficiency syndrome mimicking a soft tissue neoplasm is presented. There are few reported cases of hematogenous infection of chronic hematomas, which be difficult to differentiate from soft tissue neoplasms.     

腰椎峡部裂并滑脱CT扫描技术与CT征象分析

1资料与方法本组共35例,男25例,女10例,年龄25~56岁。其中腰痛伴单或双侧下肢疼痛及麻木19例,单纯腰痛15例,骶尾部疼痛1例,均无急性外伤史。使用美国GE公司Pro-speedAI螺旋CT机,层厚3 mm,层距3 mm。20例采用常规扫描,即L3~S1三个椎间盘,对峡部裂椎体从椎体下缘向上按椎间盘扫描     

两法提取皮质骨Ⅰ型胶原的比较研究

[目的]通过对两种方法提取的骨胶原产量及特性的比较,推荐一种较为理想的提取皮质骨I型胶原的方法。[方法]以猪皮质骨为原料,去除软组织后劈成小骨块,经酒精梯度脱水、无水乙醚脱脂、盐酸脱钙、甲醇:氯仿(1:1,v/v)再脱脂处理后,骨块软化。然后将软化的骨块在高速粉碎机上粉碎成脱钙骨基质粉。分别采用传统的碱溶法和自建的增强酶解法对脱钙骨基质粉处理,经溶解、离心、透析、冻干等操作提取皮质骨胶原。最后对增强酶解法所得产物进行鉴定,并对两种提取方法的效率及得到的产物的外观、黏度、凝胶固化性进行比较。[结果]增强酶解法所得胶原的氨基酸组成、蛋白电泳、相对分子量大小、最大光吸收波长均符合I型胶原特征;和碱溶法比较,提取率由(57.8±4.96)%提高到(94.04±0.55)%;其相同浓度(0.03%,w/v)醋酸溶液黏度测定,增强酶解法为3.71,碱提法为2.81;在37.0℃,pH值7.35～7.45条件下行凝胶固化性检测,增强酶解法所提胶原凝胶液约10min可固化形成凝胶块,而碱提法所提胶原的溶液仍为黏性液体。[结论]增强酶解法提取的骨胶原是I型胶原;相对于碱提法,该法提取效率高,所得胶原纯度高、黏性好、固化性好,是一种较好的从猪皮质骨中制备I型胶原的方法。     

Cognitive behavioral treatment for young children with obsessive-compulsive disorder.

Obsessive-compulsive disorder (OCD) is a distressing and functionally impairing disorder that can emerge as early as age 4. Cognitive behavior therapy (CBT) for OCD in youth shows great promise for amelioration of symptoms and associated functional impairment. However, the empirical evidence base for the efficacy of CBT in youth has some significant limitations, particularly as related to treating the very young child with OCD. This report includes a quantitative review of existing child CBT studies to evaluate evidence for the efficacy of CBT for OCD. It identifies gaps in the literature that, when addressed, would enhance the understanding of effective treatment in pediatric OCD. Finally, it presents a proposed research agenda for addressing the unique concerns of the young child with OCD.     

Validation of a screening questionnaire for hip and knee osteoarthritis in old people

Background  

To develop a sensitive and specific screening tool for knee and hip osteoarthritis in the general population of elderly people.     

医用硫酸钙在家兔腰椎融合模型中的成骨能力

[目的]通过与自体髂骨进行比较,评价医用硫酸钙单独应用的成骨能力,并探讨其可能的成骨机理。[方法]建立家兔腰椎后外侧融合模型,以自身作为对照,双侧横突间植骨,左侧植入硫酸钙颗粒,右侧植入自体髂骨。于术后3、6、12周行X线、CT及组织学检查。[结果]术后3周,硫酸钙尚可见残留颗粒,自体骨完全降解;植骨区域内均可见到大量的破骨细胞,并有血管纤维组织长入。术后6周,硫酸钙完全降解,影像学检查两者之间没有明显的差异,表现为局部骨密度增高,新骨成形;组织学检查两侧植骨区内均为大量的透明软骨形成,自体髂骨植骨区内可见散在的骨小梁结构。术后12周,组织学及影像学上二者没有任何的差别,两侧形成的骨组织与家兔的椎体骨组织结构相同。[结论]医用硫酸钙作为骨移植替代材料,除了具有良好的骨传导性外,硫酸钙可能还具有骨诱导性,其成骨能力与自体髂骨相当,医用硫酸钙单独应用可以取得良好的融合效果。