吉西他滨化疗联合CIK细胞治疗胰腺癌的临床分析

目的分析吉西他滨化疗联合CIK细胞治疗胰腺癌的临床效果。方法将胰腺癌患者44例随机分为观察组(吉西他滨化疗联合CIK细胞治疗)与对照组(吉西他滨化疗)。结果比较两组总有效率,差异有统计学意义(P0.05);且治疗后,观察组患者KPS评分高于对照组,差异有统计学意义(P0.05)。结论吉西他滨化疗联合CIK细胞治疗胰腺癌的临床效果显著。     

层级质量控制在外科ICU护理管理中的应用

目的探讨在外科ICU护理管理中实施层级质量控制的效果。方法选择2016年1-12月为实施前,2018年1-12月为实施后,实施前后均选取50例外科ICU患者及护理人员为研究对象,对实施前后外科ICU护理管理质量进行观察。结果实施后外科护理人员在工作人员素质、环境管理、护理质量管理及质量总分上均显著高于实施前(P<0.05);实施后ICU患者对护理管理的总满意率为98.00%,明显高于实施前总满意率86.00%,差异有统计学意义(P<0.05)。结论在外科ICU护理管理中,实施层级质量控制的护理管理模式,可显著提高ICU病房护理治疗,提高患者的满意率,值得推广。     

萸竹定眩丸治疗痰浊阻络型眩晕的疗效及对外周血FGF-21水平的影响

目的:评价萸竹定眩丸对痰浊阻络型眩晕的治疗效果及对外周血FGF-21水平的影响。方法:120例患者随机分为治疗组和对照组,对照组口服盐酸氟桂利嗪胶囊10mg,每晚1次;治疗组在对照组的基础上给予萸竹定眩丸,每次1丸,每日2次;两组均治疗14天。14天后比较两组患者临床疗效、血脂及外周血中FGF-21水平。结果:治疗组总有效率为93. 3%,对照组总有效率为66. 7%,两组总有效率比较有统计学差异(P 0. 05);治疗后两组FGF-21水平均较治疗前明显降低(P 0. 01,P 0. 05),且治疗组FGF-21水平比对照组降低更明显(P 0. 05);治疗后,两组的TC、TG、LDL-C水平均较治疗前明显下降,HDL-C水平均较治疗前明显升高(P 0. 01),且治疗组的TC水平比对照组下降更显著,HDL-C水平比对照组上升更显著(P 0. 01)。结论:萸竹定眩丸治疗椎-基底动脉供血不足性眩晕(中医辨证属痰浊阻络型眩晕)具有明确疗效;萸竹定眩丸对改善人体的糖脂代谢水平,降低TC,升高HDL-C疗效明确;萸竹定眩丸在临床应用中未发现严重不良反应,临床应用安全可靠。     

基于复杂网络技术对电针治疗乳腺增生病处方分析

目的基于复杂网络技术分析电针治疗乳腺增生病的核心穴位及配伍穴相关性,并对电针波型进行探讨。方法检索中国期刊全文数据库(CNKI)、万方数据库、维普网数据库(VIP)中从1954年1月1日到2018年12月31日公开发表的电针治疗乳腺增生病的临床中文文献,利用Excel表格工具建立电针治疗乳腺增生病数据库,利用Matlab2014a软件进行节点中心性分析和聚类分析,利用Gephi0.9.1软件制作复杂网络示意图对分析结果进行描述和展示,利用Excel表格工具制作电针治疗乳腺增生病不同波形比例饼状图并分析其规律。结果选定43条电针处方:电针治疗乳腺增生病主穴核心度前三位依次为膻中穴、足三里、肩井穴;配伍穴位核心度前三位依次为太冲穴、太溪穴、脾俞穴;经络核心度前三位依次为足阳明胃经、任脉、足少阳胆经。配伍穴位相关性分析中,相关性频度前三位组合依次为太冲穴-太溪穴、太冲穴-肾俞穴、太冲穴-三阴交。电针波形中以连续波与疏密波常见,均具有较高的临床应用价值。结论电针治疗乳腺增生病处方以足阳明胃经为最多,其次为任脉;电针波型以连续波为主。     

藿香正气汤抑制新型冠状病毒复制过程的有效成分及机制初探＊

目的 收集藿香正气汤的主要活性成分，通过分子对接及网络药理学探讨其防控新型冠状病毒肺炎（COVID-19）的有效成分及治疗机制。方法 通过基于配体-蛋白质相互作用的计算方法，以瑞德西韦为对照，探索藿香正气汤潜在治疗COVID-19的成分，并选出对接较好成分进行药理学机制预测，初探其药理学机制。结果 本研究筛选出5种与新冠病毒3CLpro结合能力强于瑞德西韦的小分子成分。网络药理学初步预测抗病毒途径可能是通过PI3K-Akt 信号通路影响病毒复制。结论 成分C1-C5与3CLpro结合良好，推测其可能是潜在的3CLpro的抑制剂，为抗病毒天然药物的开发提供了理论依据。     

F形空心钉与传统3枚平行钉治疗Pauwels Ⅲ型股骨颈骨折的疗效比较

目的:分析比较"F"形空心钉与传统倒三角3枚平行螺钉内固定治疗青壮年Pauwels Ⅲ型股骨颈骨折的临床疗效。方法：2017年1月至2020年1月收治Pauwels Ⅲ型股骨颈骨折患者38例，根据置入钉方式的不同将其分为两组，其中A组18例，采用"F"形空心钉固定，男12例，女6例，年龄37~55岁，受伤至手术时间1~3 d。B组20例，采用传统倒三角3枚平行拉力螺钉固定，男12例，女8例，年龄35~55岁，受伤至手术时间为1~3 d。比较两组患者骨折不愈合，股骨头坏死，股骨颈短缩，空心螺钉退出情况，髋关节功能Harris评分，疼痛视觉模拟评分（visual analogue scale，VAS）。结果：所有患者获得随访，时间为15~31个月。两组患者在骨折不愈合，股骨颈短缩，股骨头坏死方面差异无统计学意义（P>0.05）；两组患者在螺钉退出方面差异有统计学意义（P<0.05）。两组患者术后12个月时髋关节Harris评分及VAS评分差异均无统计学差异（P>0.05）。结论："F"形与传统倒三角3枚平行空心钉内固定治疗青壮年Pauwels Ⅲ型股骨颈骨折中短期疗效相似，但"F"形空心钉退钉率较低。     

RNA sequencing screening of differentially expressed genes after spinal cord injury

In the search for a therapeutic schedule for spinal cord injury, it is necessary to understand key genes and their corresponding regulatory networks involved in the spinal cord injury process. However, ad hoc selection and analysis of one or two genes cannot fully reveal the complex molecular biological mechanisms of spinal cord injury. The emergence of second-generation sequencing technology(RNA sequencing) has provided a better method. In this study, RNA sequencing technology was used to analyze differentially expressed genes at different time points after spinal cord injury in rat models established by contusion of the eighth thoracic segment. The numbers of genes that changed significantly were 944, 1362 and 1421 at 1, 4 and 7 days after spinal cord injury respectively. After gene ontology analysis and temporal expression analysis of the differentially expressed genes, C5ar1, Socs3 and CCL6 genes were then selected and identified by real-time polymerase chain reaction and western blot assay. The mRNA expression trends of C5ar1, Socs3 and CCL6 genes were consistent with the RNA sequencing results. Further verification and analysis of C5ar1 indicate that the level of protein expression of C5ar1 was consistent with its nucleic acid level after spinal cord injury. C5ar1 was mainly expressed in neurons and astrocytes. Finally, the gene Itgb2,which may be related to C5ar1, was found by Chilibot database and literature search. Immunofluorescence histochemical results showed that the expression of Itgb2 was highly consistent with that of C5ar1. Itgb2 was expressed in astrocytes. RNA sequencing technology can screen differentially expressed genes at different time points after spinal cord injury. Through analysis and verification, genes strongly associated with spinal cord injury can be screened. This can provide experimental data for further determining the molecular mechanism of spinal cord injury, and also provide possible targets for the treatment of spinal cord injury. This study was approved ethically by the Laboratory Animal Ethics Committee of Jiangsu Province, China(approval No. 2018-0306-001) on March 6, 2018.