A MICROPROCESSOR-DRIVEN ONE-OPERATOR FORCED-CHOICE PREFERENTIAL LOOKING APPARATUS

Forced-choice preferential looking (FPL) is a behavioural technique for the estimation of visual acuity in preverbal children. We have constructed an electromechanical FPL apparatus controlled by a microprocessor requiring just one operator. Sixty-three babies aged between six weeks and eleven months were evaluated with an overall success rate of 83% in obtaining an acuity measurement. FPL is a practical method for measurement of infant visual acuity in the ophthalmic clinic setting.     

Characterisation of the solution conformation of a cyclic RGD peptide analogue by NMR spectroscopy allied with a genetic algorithm approach and constrained molecular dynamics

The solution conformation of a cyclic RGD peptide analogue, cyclo-(S,S)-2-merrcaptobenzoate-arginine-glycine-aspartate -2-mercaptoanilide, has been determined via two independent approaches for the searching of conformational space and identification of conformations consistent with NMR and CD spectroscopic data: (i) the use of a binary genetic algorithm and (ii) a molecular dynamics simulation. Inter-proton distances were obtained via analysis of cross-peak volumes from a two-dimensional ROESY NMR spectroscopy experiment at 600 MHz and were used as constraints for the computational calculations. The mercaptoanilide amide proton resonance chemical shift had a very small temperature coefficient, indicating that this proton was hydrogen-bonded. Circular diehroism data showed that, in solution, the torsion angle about the disulfide bond was negative, consistent with one of the distinct conformations around this bond in the 200 ps molecular dynamics simulation. The backbone conformations of the structures resulting from the two different approaches were very similar.     

Gross muscle morphology and structure in spastic cerebral palsy: a systematic review

Aim This systematic review and critical evaluation of the literature was conducted to determine how gross muscle morphology and structure are altered in individuals with spastic cerebral palsy (CP). Method Electronic databases were searched for articles describing studies of muscle morphological and structural properties in individuals with spastic CP. Data describing muscle fascicle length, belly length, fascicle angle, cross‐sectional area, volume, and thickness were extracted and effect sizes were computed for comparisons between individuals with spastic CP and typically developed individuals, between the paretic and non‐paretic side in individuals with hemiplegia for all muscles examined, and across the full spectrum of gross motor function in individuals with spastic CP. Results The final yield consisted of 15 articles that met the inclusion criteria. The main finding of the review was the consistent evidence for reduced muscle belly length, muscle volume, cross‐sectional area, and muscle thickness in the comparisons between paretic and typically developed muscle and the paretic and non‐paretic muscle across a range of muscles. Interpretation Given the importance of muscle morphology and structure for generating muscle force, it is likely that the observed alterations that occur secondary to the neural lesion in individuals with spastic CP contribute to muscle weakness and the attendant loss of motor function in spastic CP.     

Interaction of atriopeptin III with lipids and detergents

Atriopeptin III, a potent natural hypotensive agent, contains little α-helical structure but substantial amounts of β-structure. The peptide can self-associate at millimolar concentrations or can associate with the anionic phospholipid, dimyristoylphosphatidylglycerol. Both of these processes are accompanied by a conformational change suggesting the formation of an increased amount of β-structure. The peptide can broaden the transition and lower the transition enthalpy of dimyristoylphosphatidylglycerol. The results demonstrate that a peptide hormone can associate with lipid largely in the form of a β-structure.     

The Effects of Tobacco Advertising on Children

The London Based Children's Research Unit (CRU) has investigated juvenile smoking initiation and advertising in 15 countries. Each country investigated differed with respect to tobacco advertising restrictions, ranging from Norway, which has had a long-term ban on all advertising of tobacco products, to Hong Kong and Japan, which had few advertising restrictions at the time of the research. Advertising was not found to significantly influence juvenile smoking initiation and it was also found to be a poor predictor of juvenile smoking prevalence. That is, countries with tobacco advertising did not have a higher level of juvenile smoking prevalence than countries without advertising.     

Coronary Flow Reserve Versus Geometric Measurements of Coronary Dimensions: Advantages and Limitations of the Functional Stenosis Assessment

This article reviews the physiopathological background of invasive functional assessment of coronary artery stenoses and the recent developments for clinical decision making based on intracoronary flow and pressure measurements. Limitations of these recordings are stressed, as well as their present clinical application to guide the decision of performing an intervention, or to assess the results of a percutaneous procedure. The additional value over (quantitative) angiography and intravascular ultrasound is discussed. Finally, the potentials of a new method of coronary flow measurement, based on the processing of the radiofrequency IVUS signal, offering combined simultaneous morphological and physiological assessments, are briefly introduced.