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71.
Phylogenetic analysis of human G9P[8] rotavirus strains circulating in Jiangsu,China between 2010 and 2016 下载免费PDF全文
Cheng Xu MD Jianguang Fu DC Jing Ai MD Jun Zhang MD Cheng Liu BD Xiang Huo MD Changjun Bao MD Yefei Zhu DM 《Journal of medical virology》2018,90(9):1461-1470
Rotavirus A (RVA) is the leading cause of acute viral gastroenteritis in children under 5 years of age worldwide. G9P[8] is a common RVA genotype that has been persistently prevalent in Jiangsu, China. To determine the genetic diversity of G9P[8] RVAs, 7 representative G9P[8] strains collected from Suzhou Children’s Hospital between 2010 and 2016 (named JS2010‐JS2016) were analyzed through whole‐genome sequencing. All evaluated strains showed the Wa‐like constellation G9‐P[8]‐I1‐R1‐C1‐M1‐A1‐N1‐T1‐E1‐H1. Furthermore, phylogenetic analysis revealed that the VP7 genes of all strains clustered into lineage G9‐III and G9‐VI. With the exception of strain JS2012 (P[8]‐4), the VP4 sequences of all strains belonged to the P[8]‐3 lineage. Sequencing further revealed that amino acid substitutions were present in the antigenic regions of the VP7 and VP4 genes of all strains. Moreover, there were multiple substitutions in antigenic sites I and II of the nonstructural protein 4 (NSP4) genes, whereas the other NSP genes were relatively conserved. In conclusion, our phylogenetic analysis of these 7 G9P[8] strains suggests that RVA varied across regions and time. Therefore, our findings suggest that continued surveillance is necessary to explore the molecular evolutionary characteristics of RVA for better prevention and treatment of acute viral gastroenteritis. 相似文献
72.
Investigating nasal cytology as a potential tool for diagnosing occupational rhinitis in woodworkers
Claudia Staffieri MD PhD Andrea Lovato MD Federica Aielli MD Martina Bortoletto MD Luciano Giacomelli BD Mariella Carrieri MD Salvatore Romeo MD PhD Paolo Boscolo-Rizzo MD Maria Cristina Da Mosto MD Giovanni Battista Bartolucci MD Gino Marioni MD Maria Luisa Scapellato MD 《International forum of allergy & rhinology》2015,5(9):814-819
73.
Defective opsonization in multiple myeloma 总被引:1,自引:0,他引:1
The mechanisms responsible for the unusual susceptibility of multiple myeloma (MM) patients to infections are incompletely defined. Since MM is associated with decreased production of normal serum proteins, we investigated the possibility that the production of opsonins might also be impaired. The neutrophil chemiluminescence assay of opsonization was used to evaluate the ability of serum from patients with MM to opsonize zymosan. It was found that sera from 18 MM patients exerted only 50% +/- 2.5% (mean +/- SEM) of the opsonic activity found in 18 control sera (p less than 0.001). In mixture experiments, untreated normal serum completely restored the opsonic activity of MM serum, suggesting a deficiency of opsonic factors rather than an inhibitor. In other mixture experiments, heat-inactivated normal serum only partially corrected the opsonic defect in MM serum. Serum from three patients had low C3 levels, and treatment of particles with these resulted in a greater opsonic defect than the patient population as a whole (p less than 0.02). No correlation between the opsonic defect and infections was established over an 18-mo period. These data suggest that MM serum lacks both heat-stable and heat-labile opsonic activity, the direct clinical significance of which remains to be clarified. However, these studies support the concept that defective host resistance in MM may be multifactoral, combining opsonic abnormalities with other defects previously described. 相似文献
74.
Synergistic cytotoxic effect of azidothymidine and recombinant interferon alpha on normal human bone marrow progenitor cells 总被引:4,自引:0,他引:4
Berman E; Duigou-Osterndorf R; Krown SE; Fanucchi MP; Chou J; Hirsch MS; Clarkson BD; Chou TC 《Blood》1989,74(4):1281-1286
Azidothymidine (AZT) and interferon alpha (IFN-alpha) are among the drugs showing strong in vitro activity against the human immunodeficiency virus type-1 (HIV-1). Each drug, however, has significant toxicity against normal marrow progenitor cells that frequently proves dose-limiting in patients. In this study, AZT and recombinant IFN-alpha 2a (rIFN-alpha 2a) were tested as single agents and in combination against normal myeloid (CFU-GM) and erythroid (BFU- E) colony forming cells in a standard methylcellulose culture assay. The data were analyzed using a quantitative computerized analysis based on the median-effect principle and the isobologram equation as described by Chou and Talalay (Adv Enz Regul 22:27, 1984). The ED90 for BFU-E and CFU-GM inhibition was then compared with previously measured in vivo plasma levels of each drug and the ED90 for the anti-HIV-1 effect in vitro. We demonstrate that (a) the drugs are strongly synergistic in inhibiting marrow progenitor cell growth and that this synergism occurs at drug levels that are within the range of measured plasma levels in phase I clinical trials, (b) BFU-E are more sensitive than CFU-GM to the inhibiting effects of AZT, rIFN-alpha 2a or both drugs in combination, (c) the drug concentrations in combination that synergistically inhibit bone marrow progenitors are much higher than those required to inhibit HIV-1 replication in vitro, and (d) the anti- HIV-1 effect for the combination of AZT and rIFN-alpha 2a was clearly superior to the effect of AZT or rIFN-alpha 2a alone as indicated by the combination index and the dose-reduction index. These data suggest that substantially lower doses of AZT and rIFN-alpha than those currently being tested in clinical trials might not only maintain a strong synergistic anti-HIV-1 effect but might also avoid significant hematologic toxicity. 相似文献
75.
76.
Uma Nahar Saikia Baijayantimala Mishra Mirnalini Sharma Ritambhra Nada BD Radotra 《Diagnostic microbiology and infectious disease》2014
Coxsackieviral myocarditis is associated with systemic involvement in neonates; however, fulminant coxsackieviral myocarditis is rare in adults, and its dissemination with fatal myocarditis involving kidneys, liver, and adrenal is further rarely reported. We report a case of fulminant myocarditis along with dissemination of coxsackievirus, which was clinically unrecognized. 相似文献
77.
78.
Imaging of the skin with 20-MHz US 总被引:4,自引:0,他引:4
79.
Guided fine-needle aspiration biopsy of nonpalpable breast lesions: calculation of accuracy values 总被引:1,自引:0,他引:1
B D Fornage 《Radiology》1990,177(3):884-885
80.
B Fornage 《Journal de radiologie》1984,65(5):375-384
Prostatic sonography is now routinely performed. Transrectal scanning provides the best imaging quality. Two different types of probes are available: radial probes for transverse scans and linear array probes for longitudinal scans. A comparative study of both types of probes has been performed in 98 patients. Complementary roles of radial and linear probes are demonstrated and the consecutive use of both probes is recommended for a complete sonographic study of prostate and surrounding structures. 相似文献