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991.
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994.
Ithasbeenprovedthatanumberofdiseasesarerelatedwithabnormalityofbloodviscosityandcoagulationinclinicalresearch.Bloodhyperviscosityandhypercoagulationcauseandaccelearatethedevelopmentofcertaindiseases,deathrateofsomeofwhicharerisingwithyears.Lookingforawaytoreducebloodviscosityandrestrainfasterandstrongercoagulationbecomesasubjectdrawingmoreattention.Theproperseofthisresearchwastofindsuchaway.Intheblood,therearechargrdRBC,WBC,PLT,inorganicions,sothattheremustbesensitiveandcomplicatedresponse… 相似文献
995.
用单克隆抗体间接免疫荧光标记法测定创伤性休克病人单个核细胞表面Mac-1、CD18的表达。结果,创伤后单个核细胞表面Mac-1、CD18表达增加,以CD18为著。它可能参与休克时微循环中白细胞贴壁粘着的发生,带来微循环血流紊乱 相似文献
996.
Yang Jueqin A Fan L Yao Z A Volgger D Xu L J Yao F G. Brünnler E. D. Albert 《Human immunology》1996,47(1-2):14
We have investigated the polymorphism of the DQA1 promoter region (QAP) and we have deduced four point (DRB1, QAP, DQA1, DQB1) haplotypes of 60 unrelated healthy Dai minority individuals using the polymerase chain reaction and Dig-ddUTP labeled oligonucleotides. A total of eight QAP alleles (QAP1.1, 1.2, 1.3, 1.4, 3.1, 3.2, 4.1 and 4.2) were detected and two QAP alleles, QAP1.5 and QAP2.1 were absent in this population. The most predominant allele was QAP1.2 with 80% allele frequency. We also found that QAP alleles are in strong linkage disequilibrium with certain alleles of the neighboring loci DQA1 and DQB1. Complete positive association was found for QAP4.1-DQA1*05, QAP4.2-DQA1*0601, QAP1.2-DR2 group, QAP3.2-DRB1*09, QAP4.1-DRB1*03. A total of 28 different four point (DRB1-QAP-DQA1-DQB1) haplotypes were deduced and the most frequent haplotypes were DRB1*1602-QAP1.2-DQA1*0102-DQB1*0502 (N = 18, H.f. = 15%) and DRB1*09-QAP3.2-DQA1*03-DQB1*03032 (N = 18, H.f. = 15%) followed by the haplotypes DRB1*1401-QAP1.3-DQA1*01-DQB1*0502, DRB1*1202-QAP4.2-DQA1*0601-DQB1*0301 and DRB1*1502-QAP1.2-DQA1*0101-DQB1*0501 with H.f. 9.1%, 6.7% and 5.0% respectively. The other 23 haplotypes were all less than 5% (H.f. 0.8%-5%). The relationship between the QAP alleles and DQA1 in the Dai minority is the same as that in the Chinese and the Caucasoid population. 相似文献
997.
孕早期宫颈分泌物中获取胎儿细胞DNA进行产前诊断的可行性 总被引:2,自引:1,他引:2
目的:探索获取胎儿细胞DNA用于产前基因诊断的可行性,及能否用于预期胎儿性别。方法:收集92例孕早期人工流产妇女宫颈分泌物和绒毛组织,提取宫颈分泌物中DNA,扩增Y染色体特异重复序列DNA。短期培养制备绒毛染色体,分析核型确定流产胎儿性别。结果:92例经绒毛染色体核型分析的人工流产胎儿中,正确预期胎儿性别72例,准确率78%。结论:采集孕早期宫颈分泌物,可获得胎儿滋养层细胞DNA用于产前诊断,其准确性和可靠性有待进一步提高。 相似文献
998.
中药髓复康对大鼠脊髓GFAP表达的影响 总被引:3,自引:0,他引:3
为了探讨中药制剂髓复康对急性脊髓半横断损伤诱发的星形胶质细胞反应性增生是否具有抑制作用,本实验选用8周龄的清洁级雄性SD大鼠54只,其中48只用于制备T12胸髓右侧半横断损伤模型,并将其随机分为髓复康组(S)和空白对照组(B)。另6只设为正常对照组(N组)。不同时间点取材,采用免疫组织化学法比较各组脊髓损伤区GFAP表达的变化。结果显示,B组在术后3、7、15和30d四个时间点GFAP免疫反应阳性细胞数和GFAP免疫阳性产物的OD值均明显高于N组,术后15d达高峰,各时间点两组之间比较均有显著性差异(P<0.05);在脊髓损伤后3d,S组的GFAP免疫反应细胞数明显少于B组(P<0.05),而GFAP免疫阳性产物的OD值与B组接近。在以后的各个时间点,S组的GFAP免疫反应细胞计数和GFAP免疫阳性产物的OD值都明显低于B组(P<0.05),而高于N组(P<0.05)。研究结果提示,髓复康能减轻大鼠脊髓半横断损伤所诱发的星形胶质细胞反应性增生。 相似文献
999.
Huang P Miao S Fan H Sheng Q Yan Y Wang L Koide SS 《Molecular human reproduction》2000,6(12):1069-1078
The YWK-II cDNA, RSD-2, encoding a sperm membrane protein was isolated from a rat testis cDNA expression library. Using the RSD-2 insert in combination with rapid amplification of cDNA ends (RACE), the corresponding human gene was isolated from a human testis cDNA expression library. The human testis cDNA, HSD-2, is 3654 bp in length and contains an open reading frame of 763 codons. Hydropathicity analysis showed that the deduced polypeptide is a single strand transmembrane protein. The deduced polypeptide has partial homology with the amyloid precursor protein (APP) and high homology with the amyloid precursor homologue, APLP2/APPH. The YWK-II gene was mapped and assigned to human chromosome locus: 11q24-25. Northern blotting of various human tissue RNAs using the HSD-2 cDNA as a probe showed that the gene is transcribed ubiquitously. The cytoplasmic domain of HSD-2 was expressed in Escherichia coli. In-vitro studies showed that the recombinant polypeptide bound to a GTP-binding protein (G(o)) and was phosphorylated by protein kinase C and cdc2 kinase. In mammalian F11 cells, the recombinant polypeptide was found to be coupled to G(o). Thus, the YWK-II component has the characteristics of a G(o)-coupled receptor and may be involved in G(o)-mediated signal transduction pathway. Protein kinase C and cdc2 kinase may regulate this pathway in spermatozoa by phosphorylating the cytoplasmic domain of the YWK-II component. 相似文献
1000.
Qihan Li Lichun Wang Chenghong Dong Yanchun Che Li Jiang Longding Liu Hongling Zhao Yun Liao Yi Sheng Shaozhong Dong Shaohui Ma 《Journal of clinical virology》2005,34(2):133-139
The pathological mechanism of SARS-CoV infection was investigated. The gene for the SARS-CoV non-structural protein 10, which is located in the open reading frame of pp1a/pp1ab gene, was synthesized and used to screen for the specific cellular gene coding for the protein interacting with this nsp10 protein in a human embryo lung cDNA library using a yeast trap method. The results indicated that apart from the two subunits of cellular RNA polymerase complex, BTF3 and ATF5, this nsp10 protein was also able to interact specifically with the NADH 4L subunit and cytochrome oxidase II. Further study revealed that the activity of the NADH-cytochrome was altered and the inner mitochondrial membrane was depolarized in the transfected human embryo lung fibroblast by the nsp10 protein gene. The cytopathic effect of the Coronavirus 229E strain appeared more extensive in these cells than in the control cells. 相似文献