全文获取类型
收费全文 | 1989篇 |
免费 | 116篇 |
国内免费 | 11篇 |
专业分类
耳鼻咽喉 | 35篇 |
儿科学 | 83篇 |
妇产科学 | 49篇 |
基础医学 | 317篇 |
口腔科学 | 23篇 |
临床医学 | 171篇 |
内科学 | 392篇 |
皮肤病学 | 107篇 |
神经病学 | 104篇 |
特种医学 | 55篇 |
外科学 | 249篇 |
综合类 | 9篇 |
一般理论 | 1篇 |
预防医学 | 122篇 |
眼科学 | 48篇 |
药学 | 283篇 |
中国医学 | 3篇 |
肿瘤学 | 65篇 |
出版年
2023年 | 8篇 |
2022年 | 23篇 |
2021年 | 52篇 |
2020年 | 24篇 |
2019年 | 34篇 |
2018年 | 55篇 |
2017年 | 38篇 |
2016年 | 39篇 |
2015年 | 38篇 |
2014年 | 79篇 |
2013年 | 76篇 |
2012年 | 115篇 |
2011年 | 102篇 |
2010年 | 68篇 |
2009年 | 84篇 |
2008年 | 128篇 |
2007年 | 85篇 |
2006年 | 86篇 |
2005年 | 64篇 |
2004年 | 66篇 |
2003年 | 67篇 |
2002年 | 58篇 |
2001年 | 57篇 |
2000年 | 51篇 |
1999年 | 57篇 |
1998年 | 20篇 |
1997年 | 14篇 |
1995年 | 16篇 |
1994年 | 12篇 |
1992年 | 32篇 |
1991年 | 27篇 |
1990年 | 30篇 |
1989年 | 29篇 |
1988年 | 34篇 |
1987年 | 46篇 |
1986年 | 31篇 |
1985年 | 24篇 |
1984年 | 33篇 |
1983年 | 28篇 |
1982年 | 15篇 |
1981年 | 14篇 |
1980年 | 7篇 |
1979年 | 21篇 |
1978年 | 9篇 |
1977年 | 10篇 |
1973年 | 11篇 |
1972年 | 15篇 |
1970年 | 10篇 |
1968年 | 7篇 |
1967年 | 7篇 |
排序方式: 共有2116条查询结果,搜索用时 15 毫秒
21.
de Pontual L Népote V Attié-Bitach T Al Halabiah H Trang H Elghouzzi V Levacher B Benihoud K Augé J Faure C Laudier B Vekemans M Munnich A Perricaudet M Guillemot F Gaultier C Lyonnet S Simonneau M Amiel J 《Human molecular genetics》2003,12(23):3173-3180
Congenital central hypoventilation syndrome (CCHS, Ondine's curse) is a rare disorder of the chemical control of breathing. It is frequently associated with a broad spectrum of dysautonomic symptoms, suggesting the involvement of genes widely expressed in the autonomic nervous system. In particular, the HASH-1-PHOX2A-PHOX2B developmental cascade was proposed as a candidate pathway because it controls the development of neurons with a definitive or transient noradrenergic phenotype, upstream from the RET receptor tyrosine kinase and tyrosine hydroxylase. We recently showed that PHOX2B is the major CCHS locus, whose mutation accounts for 60% of cases. We also studied the proneural HASH-1 gene and identified a heterozygous nucleotide substitution in three CCHS patients. To analyze the functional consequences of HASH-1 mutations, we developed an in vitro model of noradrenergic differentiation in neuronal progenitors derived from the mouse vagal neural crest, reproducing in vitro the HASH-PHOX-RET pathway. All HASH-1 mutant alleles impaired noradrenergic neuronal development, when overexpressed from adenoviral constructs. Thus, HASH-1 mutations may contribute to the CCHS phenotype in rare cases, consistent with the view that the abnormal chemical control of breathing observed in CCHS patients is due to the impairment of noradrenergic neurons during early steps of brainstem development. 相似文献
22.
23.
24.
25.
Ribault S Harper K Grave L Lafontaine C Nannini P Raimondo A Faure IB 《Journal of clinical microbiology》2004,42(5):1903-1908
Public awareness has long focused on the risks of the transmission of viral agents through blood product transfusion. This risk, however, pales in comparison to the less publicized danger associated with the transfusion of blood products contaminated with bacteria, in particular, platelet concentrates. Up to 1,000 cases of clinical sepsis after the transfusion of platelet concentrates are reported annually in the United States. The condition is characterized by acute reaction symptoms and the rapid onset of septicemia and carries a 20 to 40% mortality rate. The urgent need for a method for the routine screening of platelet concentrates to improve patient safety has long been recognized. We describe the development of a rapid and highly sensitive method for screening for bacteria in platelet concentrates for transfusion. No culture period is required; and the entire procedure, from the time of sampling to the time that the final result is obtained, takes less than 90 min. The method involves three basic stages: the selective removal of platelets by filtration following activation with a monoclonal antibody, DNA-specific fluorescent labeling of bacteria, and concentration of the bacteria on a membrane surface for enumeration by solid-phase cytometry. The method offers a universal means of detection of live, nondividing, or dead gram-negative and gram-positive bacteria in complex cellular blood products. The sensitivity is higher than those of the culture-based methods available at present, with a detection limit of 10 to 10(2) CFU/ml, depending upon the bacterial strain. 相似文献
26.
Mosaicism of a novel variant in the ANKRD11 gene in a child with a mild KBG phenotype: A case report
27.
Roger Suau Marta Vidal Ruth Aguilar Gemma Ruiz-Olalla Miquel Vázquez-Santiago Chenjerai Jairoce Augusto J. Nhabomba Ben Gyan David Dosoo Kwaku Poku Asante Seth Owusu-Agyei Joseph J. Campo Luis Izquierdo David Cavanagh Ross L. Coppel Virander Chauhan Evelina Angov Sheetij Dutta Carlota Dobaño 《Vaccine》2021,39(4):687-698
BackgroundThe evaluation of immune responses to RTS,S/AS01 has traditionally focused on immunoglobulin (Ig) G antibodies that are only moderately associated with protection. The role of other antibody isotypes that could also contribute to vaccine efficacy remains unclear. Here we investigated whether RTS,S/AS01E elicits antigen-specific serum IgA antibodies to the vaccine and other malaria antigens, and we explored their association with protection.MethodsNinety-five children (age 5–17 months old at first vaccination) from the RTS,S/AS01E phase 3 clinical trial who received 3 doses of RTS,S/AS01E or a comparator vaccine were selected for IgA quantification 1 month post primary immunization. Two sites with different malaria transmission intensities (MTI) and clinical malaria cases and controls, were included. Measurements of IgA against different constructs of the circumsporozoite protein (CSP) vaccine antigen and 16 vaccine-unrelated Plasmodium falciparum antigens were performed using a quantitative suspension array assay.ResultsRTS,S vaccination induced a 1.2 to 2-fold increase in levels of serum/plasma IgA antibodies to all CSP constructs, which was not observed upon immunization with a comparator vaccine. The IgA response against 13 out of 16 vaccine-unrelated P. falciparum antigens also increased after vaccination, and levels were higher in recipients of RTS,S than in comparators. IgA levels to malaria antigens before vaccination were more elevated in the high MTI than the low MTI site. No statistically significant association of IgA with protection was found in exploratory analyses.ConclusionsRTS,S/AS01E induces IgA responses in peripheral blood against CSP vaccine antigens and other P. falciparum vaccine-unrelated antigens, similar to what we previously showed for IgG responses. Collectively, data warrant further investigation of the potential contribution of vaccine-induced IgA responses to efficacy and any possible interplay, either synergistic or antagonistic, with protective IgG, as identifying mediators of protection by RTS,S/AS01E immunization is necessary for the design of improved second-generation vaccines.Clinical trial registration: ClinicalTrials.gov: NCT008666191. 相似文献
28.
E Voiglio F Gorry J Margonari J L Faure 《Chirurgie; mémoires de l'Académie de chirurgie》1990,116(8-9):690-698
A multiple organ block is composed of "en bloc" removed organs, connected by the vascular system, whose blood circulation is maintained by the heart, and oxygenation by the lungs under artificial ventilation. To develop the removal technique and the reanimation proceeding, and to validate this new physiological model, we used 70 Wistar rats. Some multiple organ blocks (heart-lungs, liver, pancreas, kidneys and bowel) functioned in vitro at 37 degrees C for more than 150 minutes with stable haemodynamics and preserved electrolyte and acid-base balances. Bile secretion and bilateral diuresis were present. Histopathological examination confirmed the integrity of these different organs. This physiological model could be used for the study of new preservation solutions. 相似文献
29.
Giovina De Rosa Arnerico Testa Maurizio Maurizi Maria Antonietta Satta Claudia Aimoni Alberto Artuso Evelina Silvestri Vittoria Rufini Luigi Troncone 《European journal of nuclear medicine and molecular imaging》1990,17(3-4):179-184
A young woman with a thyroid papillary carcinoma behaving as an autonomously hyperfunctioning nodule is described. Only 17 similar patients have been seen in the past 25 years. It is emphasized that hyperthyroidism does not exclude malignant disease in hot nodules. This possibility suggests that all thyroid nodules, either cold or hot, require careful management. Therefore, in at risk cases, surgery could be the most useful treatment. 相似文献
30.
Richard Bost MD Jean Hostein MD Maria Valenti Bruno Bonaz MD Nicole Payen Henri Faure Jacques Fournet MD 《Digestive diseases and sciences》1990,35(2):193-199
A quantitatively and/or qualitatively abnormal duodenogastric reflux (DGR) could be involved in the pathogenesis of nonulcer dyspepsia (NUD). The aims of this prospective study were to look for (1) a pathological DGR profile during fasting and (2) an eventual correlation between DGR profile and clinical symptoms. Twenty-six NUD patients were investigated. Seven other operated patients with a surgical procedure facilitating DGR episodes and 27 healthy volunteers served as control groups. A clinical score was determined for each patient from a standardized questionnaire. Gastric aspiration was performed for 6 hr in fasting subjects. The aspirates were pooled into 17 samples. In each sample the concentration and the output of total bile acids was determined. If the concentration was larger than 30 mol/liter in pooled samples, the concentrations of free bile acids and the distribution of the conjugated bile acids was determined. The percentage of aliquots with a total bile acid concentration larger than 50 mol/liter (without upper limit), and the percentage with a concentration larger than 2500 mol/liter was also obtained. No significant difference was demonstrated between the healthy volunteers and NUD patients, whatever the parameter considered. However, there was a significant increase in each of the quantitative parameters for the group of operated patients in comparison with the NUD patient group. No significant correlation was found between the clinical score and the DGR profile in NUD patients. Apparently, DGR episodes do not play a primary role in the pathogenesis of NUD.Part of this work was presented at the 4th European Symposium on Gastrointestinal Motility, Krakow, Poland. September 22–24, 1988.Hepatogastroenterology, 35:178, 1988 (abstract). 相似文献