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41.
Jin-Mann S Lin Dana J Brimmer Elizabeth M Maloney Ernestina Nyarko Rhonda BeLue William C Reeves 《Population health metrics》2009,7(1):18-13
Background
The Multidimensional Fatigue Inventory (MFI-20) was developed in 1995. Since then, it has been widely used in cancer research and cancer-related illnesses but has never been validated in fatiguing illnesses or in a large US population-selected sample. In this study, we sought to examine the reliability and validity of the MFI-20 in the population of the state of Georgia, USA. Further, we assessed whether the MFI-20 could serve as a complementary diagnostic tool in chronically fatigued and unwell populations. 相似文献42.
43.
Ernestina Saulle Roberta Riccioni Simona Coppola Isabella Parolini Daniela Diverio Viviana Riti Gualtiero Mariani Stefan Laufer Massimo Sargiacomo Ugo Testa 《British journal of haematology》2009,145(3):399-411
Previous studies suggested an important role for vascular endothelial growth factor (VEGF) and its receptors in postnatal haemopoiesis. However, it is unclear how VEGF receptor (VEGFR) signalling could interact with that issued from the activation of haematopoietic growth factor receptors. To elucidate this point we explored VEGF-R2 and granulocyte-macrophage colony-stimulating factor receptor (GM-CSFR) membrane localization and cell signalling in TF1-KDR cells (TF1 leukaemic cells that overexpress VEGF-R2/KDR). Activation of either GM-CSFR or VEGF-R2 was shown to determine the migration of both receptor elements (VEGF-R2 and the common β-chain of the GM-CSFR) to lipid rafts. The study of receptor phosphorylation showed that GM-CSF induced the phosphorylation of its own receptor and the transphosphorylation of VEGF-R2; on the other hand, VEGF triggered the phosphorylation of its receptor and transphosphorylated the β-chain of the GM-CSFR. Co-stimulation of TF1-KDR cells with both GM-CSF and VEGF-A resulted in massive migration of both the common GM-CSFR β-chain and VEGF-R2 to lipid rafts and sustained p38 mitogen-activated protein kinase activation. Disruption of lipid rafts inhibited the capacity of both GM-CSF and VEGF-A to activate p38. Experiments with specific p38 inhibitors showed that p38 activation was required to sustain the VEGF- and GM-CSF-dependent proliferation of TF1-KDR and the survival of primary acute myeloid leukaemia blasts. 相似文献
44.
Calderón Guzmán D Barragán Mejía G Hernández García E Juárez Olguín H 《Nutrition and cancer》2006,55(2):195-200
The aim of this study was to analyze the effect of nutritional condition and simulated exposure to ozone on Glutathione (GSH), the activity of Na+/K+ ATPase and lipid peroxidation in rat brain. Male Wistar rats were fed with 7% and 23% protein diets. Two groups were formed for each nutritional condition: one group was exposed for 15 successive days to 0.75 ppm of ozone and the other to air. Subsequently, the brain was dissected in cortex, hemispheres, cerebellum, and brainstem to measure the activity of thiobarbituric acid reactive substances (TBARS), ATPase, and levels of GSH. The activity of Na+/K+ ATPase increased in cerebellum of well-nourished rats exposed to ozone, while total ATPase and TBARS decreased in all studied areas in the malnourished groups. The levels of GSH decreased significantly (P < 0.05) in the brain of rats fed with 7% of protein diet and exposed to ozone but increased in rats fed with normal diet and exposed to ozone. These results suggest that malnutrition causes alterations in the values of Na+/K+ ATPase, total ATPase, GSH, and lipid peroxidation, while ozone contributes to these modifications. As a consequence, both variables are involved in oxidative stress in the rat brain. 相似文献
45.
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47.
M. Tartaglia Veronica Bordoni Francesco Velardi Rosaria T. Basile Ernestina Saulle Romano Tenconi Concezio Di Rocco Piero A. Battaglia 《Child's nervous system》1999,15(8):389-394
A number of craniosynostotic disorders have recently been ascribed to mutations in genes coding for the fibroblast growth
factor receptors(FGFRs). The common feature of these FGFR-associated conditions is the unilateral or bilateral premature ossification
of the coronal suture. One distinct craniosynostotic condition is trigonocephaly, which results from the premature fusion
of the metopic suture. Trigonocephaly mostly occurs as isolated cranial defect; however, the premature closure of the metopic
suture may represent a feature of more complex craniosynostotic conditions in which a progressive involvement of other cranial
sutures with age is observed. The possible involvement of mutated FGFRs in trigonocephaly was investigated in nine newborns affected by isolated premature synostosis of the metopic suture. All
except one of these cases carried no mutations in the FGFR1–3 domains indicated as hot spots for craniosynostosis-associated mutations. A T(978)C transition in the FGFR2 exon IIIa was found in a patient who had a phenotype that apparently fitted the trigonocephalic condition at birth, but
showed additional facial anomalies, which worsened progressively with age towards a Crouzon-like profile. The present finding
points out the importance, from both diagnostic and prognostic points of view, of early FGFR mutational screening in craniosynostotic conditions, even in forms that apparently do not involve closure of the coronal
suture at birth.
Received: 23 December 1998 相似文献
48.
Calderón-Guzmán D Hernández-Islas JL Espítia Vázquez IR Barragán-Mejía G Hernández-García E Del Angel DS Juárez-Olguín H 《Regulatory toxicology and pharmacology : RTP》2005,41(1):1-5
The objective of the present trial was to evaluate the effect of toluene and o-cresol, m-cresol, and p-cresol on serotonin (5-HT), its precursor 5-hydroxytryptophane (5-HTP), Na(+),K(+)-ATPase, total ATPase, and lipid peroxidation (TBARS) in rat brain. Evaluation of lipid peroxidation was realized by means of TBARS, determination of biogenic amines and enzymes assay was carried out in brain homogenate samples using HPLC and spectrophotometry, respectively. Five groups of male Wistar rats (200 g) were treated as follow: control, toluene, o-cresol, m-cresol, and p-cresol groups, which were administered 35 mg/kgi.p. of each compound, the control group was given only glycerine as vehicle. 5-HT and 5-HTP levels increased significantly (p < 0.001) in toluene and o-cresol groups. Lipid peroxidation increased significantly (p < 0.002) in all groups. A significant increase (p < 0.001) of Na(+),K(+)-ATPase was noted in the toluene and o-cresol groups, while this enzyme was reduced in the p-cresol group compared to the control group. Total ATPase showed significant differences in the p-cresol group, compared to the control group. Based in our results, it can be concluded that toluene and all cresols groups may increase lipid peroxidation and consequently induce changes in membrane fluidity. 相似文献
49.
Larsson T Marsell R Schipani E Ohlsson C Ljunggren O Tenenhouse HS Jüppner H Jonsson KB 《Endocrinology》2004,145(7):3087-3094
Mutations in the fibroblast growth factor 23 gene, FGF23, cause autosomal dominant hypophosphatemic rickets (ADHR). The gene product, FGF-23, is produced by tumors from patients with oncogenic osteomalacia (OOM), circulates at increased levels in most patients with X-linked hypophosphatemia (XLH) and is phosphaturic when injected into rats or mice, suggesting involvement in the regulation of phosphate (Pi) homeostasis. To better define the precise role of FGF-23 in maintaining Pi balance and bone mineralization, we generated transgenic mice that express wild-type human FGF-23, under the control of the alpha1(I) collagen promoter, in cells of the osteoblastic lineage. At 8 wk of age, transgenic mice were smaller (body weight = 17.5 +/- 0.57 vs. 24.3 +/- 0.37 g), exhibited decreased serum Pi concentrations (1.91 +/- 0.27 vs. 2.75 +/- 0.22 mmol/liter) and increased urinary Pi excretion when compared with wild-type littermates. The serum concentrations of human FGF-23 (undetectable in wild-type mice) was markedly elevated in transgenic mice (>7800 reference units/ml). Serum PTH levels were increased in transgenic mice (231 +/- 62 vs. 139 +/- 44 pg/ml), whereas differences in calcium and 1,25-dihydroxyvitamin D were not apparent. Expression of Npt2a, the major renal Na(+)/Pi cotransporter, as well as Npt1 and Npt2c mRNAs, was significantly decreased in the kidneys of transgenic mice. Histology of tibiae displayed a disorganized and widened growth plate and peripheral quantitative computerized tomography analysis revealed reduced bone mineral density in transgenic mice. The data indicate that FGF-23 induces phenotypic changes in mice resembling those of patients with ADHR, OOM, and XLH and that FGF-23 is an important determinant of Pi homeostasis and bone mineralization. 相似文献
50.
Evaluation of decontamination methods and growth media for primary isolation of Mycobacterium ulcerans from surgical specimens
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Yeboah-Manu D Bodmer T Mensah-Quainoo E Owusu S Ofori-Adjei D Pluschke G 《Journal of clinical microbiology》2004,42(12):5875-5876
We evaluated four decontamination methods and one nondecontamination procedure in combination with four egg-based media for the primary isolation of Mycobacterium ulcerans from tissue specimens. With mycobacterial recovery and contamination rates of 75.6 and 2.4%, respectively, the combination of the oxalic acid decontamination method with Lowenstein-Jensen medium supplemented with glycerol yielded the best results. 相似文献