全文获取类型
收费全文 | 152篇 |
免费 | 10篇 |
专业分类
儿科学 | 3篇 |
妇产科学 | 3篇 |
基础医学 | 45篇 |
口腔科学 | 1篇 |
临床医学 | 6篇 |
内科学 | 25篇 |
皮肤病学 | 11篇 |
神经病学 | 20篇 |
特种医学 | 1篇 |
外科学 | 13篇 |
预防医学 | 13篇 |
眼科学 | 1篇 |
药学 | 3篇 |
中国医学 | 1篇 |
肿瘤学 | 16篇 |
出版年
2022年 | 8篇 |
2021年 | 5篇 |
2020年 | 7篇 |
2019年 | 6篇 |
2018年 | 3篇 |
2017年 | 5篇 |
2016年 | 2篇 |
2015年 | 2篇 |
2014年 | 10篇 |
2013年 | 8篇 |
2012年 | 19篇 |
2011年 | 27篇 |
2010年 | 11篇 |
2009年 | 1篇 |
2008年 | 2篇 |
2007年 | 7篇 |
2006年 | 5篇 |
2005年 | 8篇 |
2004年 | 5篇 |
2003年 | 4篇 |
2002年 | 4篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 2篇 |
排序方式: 共有162条查询结果,搜索用时 15 毫秒
71.
Fabrizia Brisdelli Mariagrazia Perilli Doriana Sellitri Marisa Piovano Juan A. Garbarino Marcello Nicoletti Argante Bozzi Gianfranco Amicosante Giuseppe Celenza 《Phytotherapy research : PTR》2013,27(3):431-437
The purpose of this study was to investigate the effects of six lichen metabolites (diffractaic acid, lobaric acid, usnic acid, vicanicin, variolaric acid, protolichesterinic acid) on proliferation, viability and reactive oxygen species (ROS) level towards three human cancer cell lines, MCF‐7 (breast adenocarcinoma), HeLa (cervix adenocarcinoma) and HCT‐116 (colon carcinoma). Cells were treated with different concentrations (2.5–100 μM) of these compounds for 48 h. In this comparative study, our lichen metabolites showed various cytotoxic effects in a concentration‐dependent manner, and usnic acid was the most potent cytotoxic agent, while variolaric acid did not inhibit the proliferation of any of the three cell lines used. All tested lichen compounds did not exhibit free radical scavenging activity using the 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) assay. The lichen metabolites did not significantly increase the intracellular ROS level and did not prevent oxidative injury induced by t‐butylhydroperoxide in HeLa cells. To better clarify the mechanism(s) of cytotoxic effect induced by protolichesterinic acid in HeLa cells, we investigated apoptotic markers such as condensation and fragmentation of nuclear chromatin and activation of caspase‐3, 8 and 9. Our results revealed that the antiproliferative activity of 40 μM protolichesterinic acid in HeLa cells is related to its ability to induce programmed cell death involving caspase‐3, 8 and 9 activation. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
72.
Tosato S Bonetto C Di Forti M Collier D Cristofalo D Bertani M Zanoni M Marrella G Lazzarotto L Lasalvia A De Gironcoli M Tansella M Dazzan P Murray R Ruggeri M 《Neuroscience letters》2011,487(1):17-21
This study examined the inter-ocular (alternating monocular samples) and intra-ocular (monocular or binocular samples) integration during a prehensile task with a range of occlusion intervals (0-75 ms). In the first experiment, participants were uncertain regarding the impending visual condition, as well as target size and location. In the second experiment, a pre-cue on target location was provided. Data from both experiments indicated that participants modified their movement kinematics when provided with alternating monocular samples, irrespective of whether or not there was an occlusion interval. Similar adaptations were found in conditions requiring intra-ocular integration but only following the introduction of an occlusion interval. These findings are consistent with participants having a general intolerance for alternating monocular samples and as a consequence using a more cautious reach and grasp strategy. 相似文献
73.
74.
75.
76.
Altamura C Torquati K Zappasodi F Ferretti A Pizzella V Tibuzzi F Vernieri F Pasqualetti P Landi D Del Gratta C Romani GL Maria Rossini P Tecchio F 《Experimental neurology》2007,204(2):631-639
Growing evidence emphasizes a positive role of brain ipsilesional (IL) reorganization in stroke patients with partial recovery. Ten patients affected by a monohemispheric stroke in the middle cerebral artery territory underwent functional magnetic resonance (fMRI) and magnetoencephalography (MEG) evaluation of the primary sensory (S1) activation via the same paradigm (median nerve galvanic stimulation). Four patients did not present S1 fMRI activation [Rossini, P.M., Altamura, C., Ferretti, A., Vernieri, F., Zappasodi, F., Caulo, M., Pizzella, V., Del Gratta, C., Romani, G.L., Tecchio, F., 2004. Does cerebrovascular disease affect the coupling between neuronal activity and local haemodynamics? Brain 127, 99-110], although inclusion criteria required bilateral identifiable MEG responses. Mean Euclidean distance between fMRI and MEG S1 activation Talairach coordinates was 10.1+/-2.9 mm, with a 3D intra-class correlation (ICC) coefficient of 0.986. Interhemispheric asymmetries, evaluated by an MEG procedure independent of Talairach transformation, were outside or at the boundaries of reference ranges in 6 patients. In 3 of them, the IL activation presented medial or lateral shift with respect to the omega-shaped post-rolandic area while in the other 3, IL areas were outside the peri-rolandic region. In conclusion, despite dissociated intensity, the MEG and fMRI activations displayed good spatial consistency in stroke patients, thus confirming excessive interhemispheric asymmetries as a suitable indicator of unusual recruitments in the ipsilesional hemisphere, within or outside the peri-rolandic region. 相似文献
77.
Selmer KK Gilfillan GD Strømme P Lyle R Hughes T Hjorthaug HS Brandal K Nakken S Misceo D Egeland T Munthe LA Braekken SK Undlien DE 《European journal of human genetics : EJHG》2012,20(1):58-63
Next-generation sequencing (NGS) techniques have already shown their potential in the identification of mutations underlying rare inherited disorders. We report here the application of linkage analysis in combination with targeted DNA capture and NGS to a Norwegian family affected by an undiagnosed mental retardation disorder with an autosomal recessive inheritance pattern. Linkage analysis identified two loci on chromosomes 9 and 17 which were subject to target enrichment by hybridization to a custom microarray. NGS achieved 20-fold or greater sequence coverage of 83% of all protein-coding exons in the target regions. This led to the identification of compound heterozygous mutations in NAGLU, compatible with the diagnosis of Mucopolysaccharidosis IIIB (MPS IIIB or Sanfilippo Syndrome type B). This diagnosis was confirmed by demonstrating elevated levels of heparan sulphate in urine and low activity of α-N-acetyl-glucosaminidase in cultured fibroblasts. Our findings describe a mild form of MPS IIIB and illustrate the diagnostic potential of targeted NGS in Mendelian disease with unknown aetiology. 相似文献
78.
79.
Menno M. Schoonheim Jeroen J.G. Geurts Doriana Landi Linda Douw Marieke L. van der Meer Hugo Vrenken Chris H. Polman Frederik Barkhof Cornelis J. Stam 《Human brain mapping》2013,34(1):52-61
Multiple sclerosis (MS) is characterized by extensive damage in the central nervous system. Within this field, there is a strong need for more advanced, functional imaging measures, as abnormalities measured with structural imaging insufficiently explain clinicocognitive decline in MS. In this study we investigated functional connectivity changes in MS using resting‐state magnetoencephalography (MEG). Data from 34 MS patients and 28 age and gender‐matched controls was assessed using synchronization likelihood (SL) as a measure of functional interaction strength between brain regions, and graph analysis to characterize topological patterns of connectivity changes. Cognition was assessed using extensive neuropsychological evaluation. Structural measures included brain and lesion volumes, using MRI. Results show SL increases in MS patients in theta, lower alpha and beta bands, with decreases in the upper alpha band. Graph analysis revealed a more regular topology in the lower alpha band in patients, indicated by an increased path length (λ) and clustering coefficient (γ). Attention and working memory domains were impaired, with decreased brain volumes. A stepwise linear regression model using clinical, MRI and MEG parameters as predictors revealed that only increases in lower alpha band γ predicted impaired cognition. Cognitive impairments and related altered connectivity patterns were found to be especially predominant in male patients. These results show specific functional changes in MS as measured with MEG. Only changes in network topology were related to poorer cognitive outcome. This indicates the value of graph analysis beyond traditional structural and functional measures, with possible implications for diagnostic and/or prognostic purposes in MS. Hum Brain Mapp, 2013. © 2011 Wiley Periodicals, Inc. 相似文献
80.
Palmira Bernocchi Fabio Vanoglio Doriana Baratti Roberta Morini Silvana Rocchi Alberto Luisa 《Topics in stroke rehabilitation》2013,20(2):106-115
Background: After discharge from in-hospital rehabilitation, post-stroke patients should have the opportunity to continue the rehabilitation through structured programs to maintain the benefits acquired during intensive rehabilitation treatment.Objective: The primary objective was to evaluate the feasibility of implementing an home-based telesurveillance and rehabilitation (HBTR) program to optimize the patient's recovery by reducing dependency degree.Method: Post-stroke patients were consecutively screened. Data were expressed as mean ± standard deviation (SD). 26 patients enrolled: 15 were sub-acute (time since stroke: 112?±?39?days) and 11 were chronic (time since stroke: 470?±?145?days). For 3?months patients were followed at home by a nurse-tutor, who provided structured phone support and vital signs telemonitoring, and by a physiotherapist (PT) who monitored rehabilitation sessions by videoconferencing.Results: 23 patients completed the program; 16.7?±?5.2 phone contacts/patient were initiated by the nurse and 0.9?±?1.8 by the patients. Eight episodes of atrial fibrillation that required a change in therapy were recorded in two patients. Physiotherapists performed 1.2?±?0.4 home visits, 1.6?±?0.9 phone calls and 4.5?±?2.8 videoconference-sessions per patient. At least three sessions/week of home exercises were performed by 31% of patients, two sessions by 54%. At the end of the program, global functional capacity improved significantly (P?<?0.001), in particular, static (P?<?0.001) and dynamic (P?=?0.0004) postural balance, upper limb dexterity of the paretic side (P?=?0.01), and physical performance (P?=?0.002). Symptoms of depression and caregiver strain also improved.Conclusion: The home-based program was feasible and effective in both sub-acute and chronic post-stroke patients, improving their recovery, and maintaining the benefits reached during inpatient rehabilitation 相似文献