急诊切痂植皮联合rh-bFGF治疗中小面积深度烧伤的临床观察

目的 研究急诊切痂植皮联合重组人碱性成纤维细胞生长因子(rh-bFGF)治疗中小面积深度烧伤的效果.方法 选取142例中小面积深度烧伤患者为研究对象,根据治疗方式的不同,分为对照组(切痂植皮,71例)和联合组(切痂植皮+rh-bFGF,71例),比较两组手术情况、术后皮片存活率、炎性反应、过敏反应及创面组织成纤维细胞(...     

Vitamin B6 as a novel risk biomarker of fractured ankles

Ankle fractures are the most common intra-articular fractures. Osteoporosis is a common and frequent disease among the elderly with a poor prognosis and high risk of fractured ankles. However, the relationship between vitamin B6 and the incidence of fractured ankles in patients with osteoporosis is unclear.A total of 101 patients with osteoporosis were recruited. Clinical and followed-up information was recorded. And the vitamin B6, albumin, globulin, and hemoglobin in the blood were tested. Pearson''s chi-squared and spearman test were performed to analyze the correlation between fractured ankles and relative parameters. Univariate and multivariate logistic regression, receiver operating characteristic curve analysis, univariate and multivariate Cox proportional hazards regression analysis, and Kaplan–Meier method were also performed.There exist strong relation between the expression level of vitamin B6 and fractured ankle (P < .001). The expression of vitamin B6 [Odd ratio (OR) = 12.071, 95% confidence interval (CI): 4.69–31.143, P < .001] has a clear correlation with whether the patients have fractured ankles via the univariate logistic regression analysis. In terms of multivariate logistic regression level, vitamin B6 (OR = 15.384, 95% CI:5.195–45.556, P < .001) was significantly associated with fractured ankle. In addition, expression level of vitamin B6 [hazard ratio (HR) = 11.684, 95% CI: 6.419–21.267, P < .001] was significantly associated with Maintenance time from recovery to recurrence (MRTT) of patients with osteoporosis.Enhanced vitamin B6 is significantly correlated with the poor prognosis of patients with osteoporosis and the increasing incidence of fractured ankles.     

体外膜氧合器辅助下经皮冠状动脉介入治疗高危复杂冠心病

目的 观察体外膜氧合器(ECMO)辅助下经皮冠状动脉介入治疗(PCI)用于高危复杂冠心病(CHD)的价值。方法 回顾性分析48例接受ECMO辅助下PCI的高危复杂CHD患者,收集其临床资料、ECMO辅助情况、PCI情况、住院期间病情变化及随访资料,观察ECMO辅助下PCI治疗高危复杂CHD的价值。结果 ECMO辅助时间0.50~3.55 h,平均(1.57±0.65) h;对46例成功完成PCI,2例因导丝、球囊通过困难而转至心脏外科行冠状动脉旁路移植术(CABG)治疗。46例PCI成功患者中,术后肺部感染4例,脑梗死2例,输血治疗2例,支架内急性血栓形成1例,切口感染1例,腹股沟血肿1例,急性心肌梗死1例。其中4例院内死亡,包括1例术后24 h内支架内急性血栓形成、1例术后第10日急性心肌梗死导致心源性休克、2例术后1周肺部感染并发感染性休克,42例出院。随访过程中2例失访,共对40例随访35.5(6,72)个月,期间5例死于其他疾患,35例病情获得改善。结论 ECMO辅助下PCI治疗高危复杂CHD安全、可行。     

成骨细胞体外培养模型的研究进展

多种细胞参与骨的形成、重建和修复过程,其中成骨细胞的作用尤为重要,它不仅调节骨的矿化,还间接调节骨吸收功能,它的数量和功能变化直接影响多种骨骼疾病的发生、发展和预后。作为骨组织中最重要的力学感受细胞和成骨效应细胞,它的功能及调节机制已成为骨生物学研究的重中之重。目前用于研究成骨细胞生物学特性的细胞模型种类繁多,而每种模型都有其自身特点,在选择实验对象时需要对其生物学特性进行综合评估。本文将针对成骨细胞不同种属来源的原代细胞和细胞系作一简要概述。     

膀胱癌组织中MMP9、MMP2与PCNA及临床指标的研究

目的：探讨基质金属蛋白酶(MMP9、MMP2)在膀胱癌组织中的表达及意义。方法：采用免疫组织化学S—P法检测64例人膀胱移行细胞癌(TCCB)石蜡切片中MMP9、MMP2的表达。结果：64例TCCB组织中，MMP9、MMP2的阳性表达率分别为60．93％(39／64)、42．19％(27／64)；且MMP9的阳性表达率与肿瘤的病理分级和分期，以及肿瘤的大小及PCNA的阳性表达率相关；与年龄、性别、肿瘤数目无关。结论：MMP9的表达可以作为估计TCCB的预后及术后综合治疗的生物学指标。     

南通汉族群体17个常染色体STR基因座遗传多态性

目的:研究南通汉族群体17个常染色体STR基因座遗传多态性。方法:利用AGCU 17+1荧光检测试剂盒,对467例南通汉族无关个体的17个常染色体STR基因座(CSF1PO、D13S317、D16S539、D18S51、D19S433、D21S11、D2S1338、D3S1358、D5S818、D6S1043、D7S820、D8S1179、FGA、Penta E、TH01、TPOX、vWA)进行扩增,用ABI 3100xl遗传分析仪和GeneMapper ID v3.2软件进行STR分型分析,用Cervus 3.0软件进行等位基因频率和遗传学参数统计分析,并检验Hardy-Weinberg。结果:南通汉族群体中,17个STR基因座基因频率(GF)在0.001 1⁰.509 6之间,杂合度(H)在0.636 00.509 6之间,杂合度(H)在0.636 0⁰.933 6之间,期望杂合度(He)在0.626 80.933 6之间,期望杂合度(He)在0.626 8⁰.921 9,个体识别能力(DP)在0.797 40.921 9,个体识别能力(DP)在0.797 4⁰.986 5之间,非父排除率(PE)在0.33630.986 5之间,非父排除率(PE)在0.3363⁰.8645之间,多态性息含量(PIC)在0.563 50.8645之间,多态性息含量(PIC)在0.563 5⁰.915 4之间,结果经χ2检验均符合Hardy-Weinberg平衡定律(P>0.05)。结论:南通汉族群体17个STR基因座具有较高多态性,能够满足南通汉族人群个体识别和亲权鉴定的需要,也可在群体遗传学等相关研究和实践中有较高的价值。     

Earthquake experience interference effects in a modified Stroop task: An ERP study

The effects of the modified Stroop task on ERP were investigated in 20 subjects who had experienced the Sichuan earthquake and a matched control group. ERP data showed that Incongruent stimuli elicited a more negative ERP deflection (N300–450) than did Congruent stimuli between 300 and 450 ms post-stimulus in the earthquake group but not found in the control group, and the N300–450 might reflect conflict monitor (the information of color and meaning do not match) in the early phase of perception identification due to their sensitivity to the external stimulus. Then, Incongruent stimuli elicited a more negative ERP deflection than did Congruent stimuli between 450 and 650 ms post-stimulus in both the groups. Dipole source analysis showed that the N450–650 was mainly generated in the ACC contributed to this effect in the control group, which might be related to monitor and conflict resolution. However, in the earthquake group, the N450–650 was generated in the thalamus, which might be involved in inhibiting and compensating of the ACC which may be related to conflict resolution process.     

齐拉西酮与利培酮治疗女性精神分裂症对照研究

目的探讨齐拉西酮与利培酮治疗女性精神分裂症患者的临床疗效及安全性。方法将64例精神分裂症患者随机分为两组,每组32例,研究组口服齐拉西酮治疗,对照组口服利培酮治疗,观察8周。于治疗前及治疗第2周、4周、8周末采用阳性与阴性症状量表评定临床疗效,副反应量表评定不良反应。结果治疗后两组阳性与阴性症状量表总分及各因子分均较治疗前有显著下降（P〈o．01）,同期两组问比较均无显著性差异（P〉0．05）。治疗8周末,研究组显效率71．88％,有效率为84．38％;对照组分别为84．38％,93．75％。两组疗效无显著性差异（P〉0．05）。两组不良反应均较轻微,研究组主要表现为头晕和锥体外系反应,对照组主要表现为体质量增加。结论齐拉西酮与利培酮治疗女性精神分裂症疗效均显著且相当,安全性高,依从性好。     

骨桥蛋白和核因子-κB在侵袭转移性胃癌中的表达

目的:探讨骨桥蛋白(Osteopontin OPN)和核因子-κB(Nuclear factor-κB p65 NF-κB p65)在胃癌侵袭转移中的作用。方法:采用免疫组织化学SABC方法检测12例非癌胃组织和72例胃癌组织中OPN和NF-κB p65的表达及分布。结果:1)OPN和NF-κB p65的阳性表达率在12例非癌胃组织中均为0,在30例无淋巴结转移胃癌组织中分别为43.30%和40.00%,在26例有淋巴结转移胃癌组织中分别为76.92%和73.07%,在16例有淋巴结转移且伴胃外器官转移的胃癌组织中分别为87.50%和81.25%。2)OPN和NF-κB p65的阳性表达率在淋巴结转移伴有胃外器官转移与无淋巴结转移的胃癌组织中有明显差异(P_a=0.004,P_c=0.007),在有淋巴结转移与无淋巴结转移的胃癌组织中有明显差异(P_b=0.011,P_d=0.013)。3)OPN与NF-κB的阳性表达有差异且相关(P=0.042,r=0.67)。结论:胃桥蛋白和核因子-κB在胃癌组织及其转移淋巴结和组织中高表达,提示其与胃癌的发生和转移有关。