全文获取类型
收费全文 | 11660篇 |
免费 | 699篇 |
国内免费 | 69篇 |
专业分类
耳鼻咽喉 | 65篇 |
儿科学 | 360篇 |
妇产科学 | 249篇 |
基础医学 | 1987篇 |
口腔科学 | 111篇 |
临床医学 | 1118篇 |
内科学 | 2883篇 |
皮肤病学 | 191篇 |
神经病学 | 1029篇 |
特种医学 | 299篇 |
外科学 | 1588篇 |
综合类 | 35篇 |
现状与发展 | 1篇 |
一般理论 | 9篇 |
预防医学 | 711篇 |
眼科学 | 78篇 |
药学 | 740篇 |
中国医学 | 21篇 |
肿瘤学 | 953篇 |
出版年
2023年 | 44篇 |
2022年 | 87篇 |
2021年 | 241篇 |
2020年 | 149篇 |
2019年 | 238篇 |
2018年 | 278篇 |
2017年 | 183篇 |
2016年 | 219篇 |
2015年 | 240篇 |
2014年 | 377篇 |
2013年 | 508篇 |
2012年 | 845篇 |
2011年 | 898篇 |
2010年 | 492篇 |
2009年 | 499篇 |
2008年 | 807篇 |
2007年 | 864篇 |
2006年 | 852篇 |
2005年 | 913篇 |
2004年 | 867篇 |
2003年 | 809篇 |
2002年 | 770篇 |
2001年 | 86篇 |
2000年 | 39篇 |
1999年 | 110篇 |
1998年 | 168篇 |
1997年 | 123篇 |
1996年 | 89篇 |
1995年 | 94篇 |
1994年 | 74篇 |
1993年 | 77篇 |
1992年 | 40篇 |
1991年 | 40篇 |
1990年 | 36篇 |
1989年 | 29篇 |
1988年 | 28篇 |
1987年 | 35篇 |
1986年 | 27篇 |
1985年 | 8篇 |
1984年 | 16篇 |
1983年 | 19篇 |
1982年 | 15篇 |
1981年 | 22篇 |
1980年 | 27篇 |
1979年 | 9篇 |
1978年 | 7篇 |
1977年 | 8篇 |
1976年 | 5篇 |
1975年 | 3篇 |
1968年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
91.
92.
93.
Wollmer MA Papassotiropoulos A Streffer JR Grimaldi LM Kapaki E Salani G Paraskevas GP Maddalena A de Quervain D Bieber C Umbricht D Lemke U Bosshardt S Degonda N Henke K Hegi T Jung HH Pasch T Hock C Nitsch RM 《Psychiatric genetics》2002,12(3):155-160
Tissue inhibitor of metalloproteinases 1 (TIMP-1) inhibits several proteinases including a disintegrin and metalloproteinase 10 (ADAM10), a major alpha-secretase that cleaves the beta-amyloid precursor protein within its amyloidogenic Abeta domain. The gene encoding TIMP-1 (TIMP 1) maps to the short arm of the X chromosome, in a region previously suggested as conferring genetic susceptibility for Alzheimer's disease (AD). To determine whether genetic variability of TIMP 1 contributes to the pathogenesis of AD, we analysed one single nucleotide polymorphism within TIMP 1 and one single nucleotide polymorphism in the 5'-untranslated region of TIMP 1 in patients with AD and control subjects from two independent and ethnically different populations. We did not observe any association between TIMP 1 genotypes and the diagnosis of AD in men or women. We also measured TIMP-1 protein levels in the cerebrospinal fluid of patients with AD, healthy control subjects, and patients with other neurological disorders. TIMP-1 levels were similar in all groups. In addition, no significant differences were observed after stratification for TIMP 1 genotypes. Our data show that neither genetic variability nor protein levels of TIMP-1 are associated with AD. 相似文献
94.
Faucher M Guillot C Marqueste T Kipson N Mayet-Sornay MH Desplanches D Jammes Y Badier M 《Pflügers Archiv : European journal of physiology》2005,450(1):45-52
This study tried to differentiate the consequences of chronic hypoxia on the electrophysiological and physiological properties and the histological characteristics of slow and fast muscles in rats. Animals inhaled a 10% O2 concentration for a 1-month period. Then, slow [soleus (SOL)] and fast [extensor digitorum longus (EDL)] muscles were analyzed in vitro by physiological and electrophysiological measurements and histological analyses. The results were compared to those obtained in corresponding muscles of an age-matched normoxic group. After exposure to hypoxia: (1) in SOL, there was a tendency to elevated Fmax, a significant increase in twitch force and tetanic frequency and a shortening of M-wave duration, and a reduced percentage of type I fibres, whereas the proportion of type IIa fibres doubled; (2) in EDL, Fmax and tetanic frequency were lowered, the muscle became less resistant to fatigue, and the proportion of type IId/x fibres was halved. Then, after 1 month of hypoxia, in the SOL muscle, both the contractile and histological properties resemble those of a fast muscle. By contrast, the EDL became slower, despite its histology was modestly affected. Reduced muscle use in hypoxia could explain the tendency for deteriorating adaptations in EDL, and the faster properties of SOL could result from hypoxia-induced inhibition of the growth-related fast-to-slow shift in muscle fibre types. 相似文献
95.
TaqMan amplification system with an internal positive control for HCV RNA quantitation. 总被引:6,自引:0,他引:6
96.
Wuyts W Roland D Lüdecke HJ Wauters J Foulon M Van Hul W Van Maldergem L 《American journal of medical genetics》2002,113(4):326-332
Multiple exostoses represent a genetically heterogeneous disorder that may occur isolated or as part of a complex contiguous gene syndrome such as Langer-Giedion syndrome on chromosome 8 and the proximal 11p deletion syndrome on chromosome 11. Here we describe a boy with multiple exostoses, hypertrichosis, mental retardation, and epilepsy due to a de novo deletion on chromosome 8q24. Molecular analysis revealed that the deletion interval overlaps with the Langer-Giedion syndrome and involves the EXT1 gene and additional genes located distal to EXT1, but probably not encompassing the TRPS1 gene located proximal to EXT1. 相似文献
97.
Chapiro E Feldmann D Denoyelle F Sternberg D Jardel C Eliot MM Bouccara D Weil D Garabédian EN Couderc R Petit C Marlin S 《European journal of human genetics : EJHG》2002,10(12):851-856
Hearing impairment is the most frequent sensory defect in children, with a genetic basis in about 50% of cases. Several point mutations and deletions in mitochondrial DNA (mtDNA) have been identified in non-syndromic sensorineural hearing loss (NSSNHL). Beside the frequent A1555G mutation, a number of mutations in tRNAs have been reported recently, but their incidence remains unknown. We identified the T7511C mutation in the tRNASer(UCN) gene in two French families with isolated deafness. Maternal transmission was obvious in both. The 15 patients with hearing impairment exhibited a variable disease phenotype in terms of onset, severity, and progression. T7511C was present in all the patients screened. Homoplasmic and heteroplasmic levels were observed and did not correlate with the severity of the disease. T7511C was also present in 12 hearing offspring of the oldest deaf mothers, confirming the existence of modulatory factors. Our data suggest that this mtDNA mutation should be screened for in all cases of familial NSSNHL compatible with maternal transmission. 相似文献
98.
99.
David Laurin Eric Spierings Lars T van der Veken Abdelbasset Hamrouni J H Frederik Falkenburg Gerard Souillet Corine Vermeulen Annie Farre Claire Galambrun Dominique Rigal Yves Bertrand Els Goulmy Assia Eljaafari 《Biology of blood and marrow transplantation》2006,12(11):1114-1124
In vitro stimulation of human female T cells with male HLA-identical dendritic cells resulted in the generation of HLA-DQB1*0501/0502-restricted minor histocompatibility H-Y antigen-specific CD4(+) T cell clones. Two clones generated from different HLA-identical pairs were analyzed. Use of HLA-DQ5-expressing female Epstein-Barr virus transformed B lymphoblastoid cell lines transfected with various H-Y genes and loaded with overlapping peptides demonstrated that both T cell clones are specific for a peptide encoded by DDX3Y. Previously, an HLA-DQ5-restricted T cell clone specific for the same peptide was isolated from a patient with graft-versus-host disease. Thus, we compared the T cell receptor (TCR) rearrangements of the 2 in vitro generated T cell clones and the ex vivo isolated T cell clone. All 3 clones shared the same TCRBV5-4* gene segment and 2 of 3 clones also used similar TCR-Valpha segments. Our results suggest that T cells recognizing the HLA-DQ5/DDX3Y T cell epitope might be characterized by a relatively limited TCR-beta repertoire. The differences in the junctional TCR-beta region had no effect on the antigen specificity, but altered the capacity of the TCR to distinguish the HLA-DQ5/DDX3Y complex from its allelic counterpart. The results also demonstrate that in vitro stimulation of T cells with allogeneic HLA-identical dendritic cells may facilitate the characterization of in vivo, potentially relevant HLA class II-restricted minor H epitopes. 相似文献
100.
Denis Heresbach Mehdi Alizadeh Dominique Reumaux Jean-Frdric Colombel Maryvonne Delamaire Pierre-Marie Danze Michel Gosselin Bernard Genetet Jean-Franois Bretagne Gilbert Semana 《Journal of autoimmunity》1996,9(6):777-784
The pathogeny of ulcerative colitis (UC) is not yet elucidated, but some arguments suggest the implication of genetic factors. Among the candidate genes, those encoding for HLA class II genotypes have been extensively studied in UC; however, discordant data may be imputable to heterogeneity, characterized by immunological markers such as atypical ANCA (p-ANCA), or to inclusion of more or less intractable UC. The aim of our study is to evaluate the interest of HLA class II and TAP genetic markers to identify different clinical forms of UC, according to p-ANCA status. Unrelated patients with a history of UC (n=91) and healthy control subjects with no personal or family history of inflammatory bowel diseases (IBD) (n=200) were included. HLA-DRB103 was less frequent in UC patients than in healthy controls (8% vs 28%,PC<0.03). No association was found with any TAP genotypes. Moreover, there was no association with the HLA-DR2 specificity, either in the entire group of UC patients (38% vs 28%) or in the p-ANCA-positive subgroup of patients (30%). The most consistent finding in the present study is that some genetic markers may characterize intractability in UC patients. HLA-DR2 was associated with poor prognosis, regardless of p-ANCA status. In HLA-DR2 and non-HLA-DR2 groups, colectomy was done in 55% and 27% of patients, respectively (PC<0.05). Furthermore, in non-HLA-DR2 patients, p-ANCA could be of interest to characterize those with more severe prognosis. Our results confirm the interest of genetic studies to define UC genetic susceptibility, taking into account intractability of the disease. They do not support the hypothesis that p-ANCA is a subclinical marker of genetic susceptibility to UC. 相似文献