An antibody targeting the N-terminal domain of SARS-CoV-2 disrupts the spike trimer

The protective human antibody response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) focuses on the spike (S) protein, which decorates the virion surface and mediates cell binding and entry. Most SARS-CoV-2 protective antibodies target the receptor-binding domain or a single dominant epitope (“supersite”) on the N-terminal domain (NTD). Using the single B cell technology called linking B cell receptor to antigen specificity through sequencing (LIBRA-Seq), we isolated a large panel of NTD-reactive and SARS-CoV-2–neutralizing antibodies from an individual who had recovered from COVID-19. We found that neutralizing antibodies against the NTD supersite were commonly encoded by the IGHV1-24 gene, forming a genetic cluster representing a public B cell clonotype. However, we also discovered a rare human antibody, COV2-3434, that recognizes a site of vulnerability on the SARS-CoV-2 S protein in the trimer interface (TI) and possesses a distinct class of functional activity. COV2-3434 disrupted the integrity of S protein trimers, inhibited the cell-to-cell spread of the virus in culture, and conferred protection in human angiotensin-converting enzyme 2–transgenic (ACE2-transgenic) mice against the SARS-CoV-2 challenge. This study provides insight into antibody targeting of the S protein TI region, suggesting this region may be a site of virus vulnerability.     

参麦注射液联合川芎嗪对脓毒症小鼠肝脏的保护作用

目的探讨参麦联合川芎嗪注射液对脓毒症小鼠肝脏的保护作用。方法采用盲肠结扎穿孔(Cecal ligation and puncture,CLP)方法诱导形成脓毒症。将雄性成年的昆明小鼠随机分为5组,分别是假手术组、模型组、参麦注射液治疗组、川芎嗪注射液治疗组和参麦联合川芎嗪注射液治疗组。CLP术前24,12,1 h各组小鼠分别腹腔注射参麦注射液和川芎嗪注射液或者等计量的生理盐水。观察CLP造模后24 h各组小鼠血清中肿瘤坏死因子α(TNF-α)、白介素6(IL-6)含量、谷丙转氨酶(ALT)、谷草转氨酶(AST)活性以及肝脏组织中丙二醛(MDA)、髓过氧化物酶(MPO)水平,并观察各组小鼠的存活率及其肝脏组织的病理变化。结果参麦、川芎嗪及参麦联合川芎嗪三组药物治疗组的脓毒症小鼠其死亡率、血清中炎症因子(TNF-α、IL-6)含量、肝脂质过氧化(MDA、MPO)水平、ALT/AST及其肝脏病理损伤程度均较模型组明显减轻,尤以参麦联合川芎嗪给药治疗组疗效最为显著。此外,联合给药治疗组血清中的炎症因子(TNF-α、IL-6)水平较单一用药组显著降低。结论参麦联合川芎嗪注射液较单一用药能够更有效地抑制炎症反应,提高脓毒症小鼠的存活率、减轻肝脏损伤。     

痔瘘熏洗凝胶剂药效学初步研究

目的:观察痔瘘熏洗凝胶剂的抗炎、镇痛效果.方法:选取雌性大鼠分组后通过建立角叉菜胶肛周肿胀模型,观察痔瘘熏洗凝胶剂对实验性痔疮的抗炎作用;选取雌性小鼠分组后通过热板法观察痔瘘熏洗凝胶剂的镇痛作用.结果:痔瘘熏洗剂及其改进剂型--痔瘘熏洗凝胶剂对角叉菜胶所致大鼠肛周肿胀均有显著的抑制作用,对热传导引起的疼痛有确切的镇痛作...     

Endocrine profile of follicles containing oocytes with subsequent polyploid fertilization

In an attempt to identify oocytes at risk for polypronuclear fertilization, follicular fluids were obtained retrospectively that contained oocytes that fertilized normally and abnormally. Whenever possible, each patient served as her own control during the same stimulation cycle. Twenty-six of 169 patients had oocytes that became polypronuclear, and of those 26, 21 had oocytes that fertilized and cleaved normally. Follicular fluids were analyzed for estradiol, progesterone, androstenedione, transferrin, and insulin. Insulin levels were noted to be significantly elevated (P less than 0.05) in the polypronuclear group when compropose that insulin, a known growth factor for granulosa cells cultured in vitro, when present in excessive concentrations may predispose to polypronuclear fertilization.     

生长抑素联合清营汤治疗重症急性胰腺炎临床疗效

目的探讨早期应用生长抑素联合清营汤治疗重症急性胰腺炎患者的近期疗效。方法68例重症急性胰腺炎患者,随机分成两组。治疗组(n=34),对照组(n=34),并观察两组患者的近期疗效。结果重症急性胰腺炎早期联用清营汤组治疗1周后,患者APACHEⅡ评分明显下降,两周复查CT显示联用清营汤组,患者急性胰腺炎的CT严重度指数(CT severity index,CTSI)评分明显下降。而单纯施他宁组患者治疗一周后APACHEⅡ评分略有下降,两周复查CT显示患者急性胰腺炎的CTSI评分略下降。两组结果相比差异显著(P<0.05)。结论早期应用生长抑素联合清营汤治疗重症急性胰腺炎可改善临床症状,提高疗效,减少并发症,降低死亡率。     

Altered in vitro immune response to hypoxia-treated normal peritoneal fibroblasts

Hypoxia treatment of normal peritoneal fibroblasts results in alterations that are characteristic for fibroblasts obtained from postoperative peritoneal adhesion tissue. In this study, we have shown that hypoxia treatment enhances normal peritoneal fibroblast elimination by lymphokine-activated killer cells to the level of adhesion tissue fibroblasts, suggesting a pivotal role of hypoxia in the adhesion development.     

Utility of screening for other causes of infertility in women with "known" polycystic ovary syndrome

We investigated the prevalence of abnormal screening results (questionnaire and testing for other causes of oligo-ovulation, male or tubal factor infertility) in a group of 1,313 oligo-ovulatory women (included and excluded subjects) whose condition was screened for inclusion in the Pregnancy in Polycystic Ovary Syndrome trial, a multicenter clinical trial that was conducted at 13 sites in the United States. Other than failure to demonstrate laboratory evidence of hyperandrogenemia, the most common reasons for subject exclusion were persistent oligospermia and tubal factor infertility.