Genetic analysis of 18 families with tuberous sclerosis complex

neurogenetics - Tuberous sclerosis complex (TSC) is mainly caused by variants in TSC1 and TSC2, which encodes hamartin protein and tuberin protein, respectively. Here, we report clinical and...     

医护治一体化工作模式对全膝关节置换术患者生活质量的影响

目的探讨医护治一体化工作模式对全膝关节置换术后患者生活质量的影响。方法2018年10月至2019年10月甘肃省中医院骨科行初次全膝关节表面置换术的患者90例。采用数字表法将患者随机分为2组。观察组45例实施专病医护治一体化工作模式;对照组45例实施常规临床工作模式。采用美国膝关节学会评分(KSS)、疼痛视觉模拟评分(VAS)、膝关节活动度(ROM)、日常生活活动能力量表(ADL)评分进行比较。采用spss 23.0软件对数据进行处理。结果入院当天患者KSS、VAS、ROM、ADL评分两组间比较差异无统计学意义(P>0.05)。术后第3天VAS、ROM、ADL评分观察组优于对照组(P<0.05),KSS评分两组间比较差异无统计学意义(P>0.05)。术后第7天,KSS、VAS、ROM、ADL观察组优于对照组(P<0.05)。术后2周KSS、VAS、ROM、ADL观察组优于对照组(P<0.05),KSS功能评分两组间比较差异无统计学意义(P>0.05)。术后1个月KSS、VAS、ROM、ADL观察组优于对照组(P<0.05),KSS两组间比较差异无统计学意义(P>0.05)。术后3、6个月KSS、KSS、VAS、ROM、ADL观察组优于对照组(P<0.05)。结论专病医护治一体化工作模式对全膝关节置换术患者膝关节功能、疼痛程度、膝关节活动能力,日常活动能力都有明显改善,有利于提高患者生活质量。     

基于OSCE模式的考核框架在院级骨科专科护士培训中的应用

目的 探讨基于OSCE模式的考核框架在院级骨科专科护士培训中的应用效果。方法 选取2020年10月至2021年1月济宁医学院附属医院进行骨科专科技术培训的骨科护理人员为研究对象,按照随机数字表法分为常规培训组和OSCE模式培训组,每组35人,常规培训组护士接受传统模式的培训,即理论知识教学和临床技能培训;OSCE模式培训组护士开展基于OSCE模式考核的培训形式。比较两组护士理论知识成绩、技能操作成绩、岗位胜任力以及培训满意度。结果 培训后,OSCE模式培训组的理论知识和技能考核的平均成绩、岗位胜任能力以及培训的满意度均高于常规培训组,差异有统计学意义(P<0.05)。结论 基于OSCE模式的培训有利于提高骨科专科护士的岗位胜任力,实现由操作型护士转变为综合能力型护士的目的,提升护理人才质量,值得临床推广。     

Dysregulated miR‐98 Contributes to Extracellular Matrix Degradation by Targeting IL‐6/STAT3 Signaling Pathway in Human Intervertebral Disc Degeneration

Intervertebral disc degeneration (IDD) is associated with dysregulated expression of microRNAs (miRNAs). However, the precise molecular mechanisms underlying this disorder remain unclear. Therefore, we tested the hypothesis that miRNAs modulate IDD through effects on the IL‐6/STAT3 signaling pathway, a potential regulator of IDD. The miRNA expression profile was determined in nucleus pulposus (NP) tissues from patients with IDD and controls, employing miRNA microarray and quantitative real‐time PCR (RT‐qPCR). Biological functions of differential expression miRNAs were further investigated using immunofluorescent staining. Luciferase reporter assays and Western blotting were performed to determine miRNA targets. We identified 41 miRNAs that were differentially expressed in patients compared with controls. Following RT‐qPCR confirmation, miR‐98 was significantly downregulated in degenerative NP tissues. Moreover, its level was inversely correlated with grade of disc degeneration. Through gain‐of‐function and loss‐of‐function studies, miR‐98 was shown to significantly promote type II collagen expression in NP cells. Interleukin‐6 (IL‐6) was identified as a target of miR‐98. Knockdown of IL‐6 induced effects on NP cells similar to those induced by miR‐98. In contrast, IL‐6 treatment abrogated the effects induced by miR‐98 upregulation. Moreover, miR‐98 dramatically suppressed expression of STAT3 target gene, MMP2. IL‐6 treatment antagonized this effect, whereas knockdown of IL‐6 by IL‐6 short hairpin RNA (shIL‐6) induced inhibitory effects on the expression of p‐STAT3 and its main target genes, similar to miR‐98. The mRNA level of IL‐6 was inversely correlated with that of miR‐98 in degenerative NP tissues. These results suggest the downregulation of miR‐98 could promote IDD through the IL‐6/STAT3 signaling pathway. Our findings also highlight miR‐98 as a novel hopeful therapeutic target for IDD. © 2015 American Society for Bone and Mineral Research.