The absorption of oleic acid in the bile fistula rat

A technique is described for collecting thoracic duct lymph in a portable glass saddle from an unrestrained rat.

Uniformly labelled oleic acid in various physical states was given to rats with thoracic duct and bile fistulae to study the influence of bile salts on the amount absorbed and on the route of transport and esterification after absorption. It is suggested that in addition to their emulsifying action bile salts may stimulate the esterification of absorbed oleic acid by the intestinal mucosa.

     

The Effect of Methylene Blue Addition to Whole Blood during Prolonged Storage

Abstract. Addition of methylene blue (MB) in concentrations of 10^–3, 10^–4 and 10^–5 m to CPD blood resulted in lowered levels of glutathione and glutathione reductase. The levels of adenosine triphosphate were not affected by the addition of MB. However, the 2,3-diphosphoglycerate levels remained higher than the control values after 21–42 days of storage at all MB concentrations used. Hemoglobin function was better maintained by the two lower concentrations of MB during most of the storage period. The highest concentration had an adverse effect, lowering the p₅₀ below the control level in CPD.     

The effect of different calcium antagonists and a calcium agonist on the metabolism of propranolol by isolated rat hepatocytes

Summary— The influence of the dihydropyridine calcium entry blockers nicardipine, amlodipine, nifedipine, isradipine and of the dihydropyridine calcium entry promotor BAY K 8644 on the disappearance rate of propranolol by isolated rat hepatocytes was compared to the effect of diltiazem and verapamil, two non-dihydropyridine calcium channel blockers and known inhibitors of hepatic cytochrome P450 mixed function oxidases. All compounds dose-dependently inhibited the disappearance rate of propranolol. Nicardipine and isradipine were more potent in inhibiting the disappearance rate of propranolol than the other dihydropyridines and than diltiazem and verapamil. The inhibitory effect of nicardipine on the disappearance rate of propranolol was not stereoselective and was not influenced by age.