Whole CanA Gene Amplification of Helicobacter pylori and Its Fingerprinting by Restriction Fragment Length Polymorphism

To set up a method of amplification for the whole CagA gene of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism(RFLP),nested PCR was employed in combination with TD-PCR to amplify the gene and EcoRI and Hind Ⅲ were used to generate the RFLP fingerprinting.Target DNA fragments from 13 of 20 samples were successfully amplified and the relevant RFLP fingerprintings were obtained.It is concluded that the method can be used to amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile.     

Chronic recurrent multifocal osteomyelitis and psoriasis—A report of a new association and review of related disorders

In summary, we have described two patients with CRMO and psoriasis, and have reviewed the musculoskeletal manifestations associated with pustular eruptions of the palms and soles. In view of the frequent occurrence of PPP in patients with CRMO, we suggest that the occurrence of psoriasis in our two patients is more than coincidence, and that noninfectious, inflammatory lesions of bone may be another musculoskeletal manifestation of psoriasis. This rare association, as well as the association of PPP with disorders associated with new bone formation, may shed new insights on the relatively common finding of periosteal elevation associated with psoriatic arthritis and the occasional severe juxta-articular osteolytic destructive bone lesions seen in psoriatic arthritis.     

Effect of exercise on repetitive nerve stimulation studies: new appraisal of an old technique.

Repetitive nerve stimulation (RNS) is a simple and rapid method for evaluation of neuromuscular transmission defects. Although the effect of exercise in conjunction with RNS is well recognized, it has not been standardized in actual patient and control groups. In a prospective study over a period of 1 year, the authors evaluated the effect of exercise in conjunction with RNS in comparison with conventional 3-Hz RNS at rest in the clinical setting. Fifty-four patients who were referred for possible neuromuscular transmission disorders, in addition to 35 healthy control subjects, were studied. Amplitude and area decremental responses with RNS at rest and after 20 seconds of maximal exercise at 1-minute intervals up to 3 minutes were evaluated. The use of RNS with exercise resulted in additional diagnostic yield of up to 36.4% compared with conventional 3-Hz RNS at rest. The standardized use of exercise with RNS is advocated for increasing its diagnostic yield in the neurophysiologic laboratory.     

应用经皮内镜下胃造口术(PEG)实施肠内营养

目的 介绍应用经皮内镜下胃造口术(PEG)实施肠内营养支持。方法 在内镜引导下行胃造口术．7例病人均予以肠内营养。结果 7例病人置管8次，成功率100％，6例经治疗后恢复情况好，无严重并发症。结论在肠内营养输注途径中PEG具有创伤小、时间短、经济，安全等特点。易于护理和长期使用。     

我院病案管理的几个问题及改进建议

病案是医疗工作的综合体现,是医院医护质量、技术水平、管理水平综合评价的重要依据,可为医疗、教学、科研提供丰富的科学资料,并能为医疗事故、纠纷提供可靠的法律依据.因此,我院坚持不懈地采取各种措施加强对病案的管理.病案管理工作是我院实施医院管理,评价医院工作效率,进行工作预测和决策的基础,而且还为我院教学和科研提供可靠的临床资料,随着现代医学的进一步发展,医疗技术水平亦在不断递增,我们要保质保量地完成病案管理的日常事务工作,就有必要探讨提高病案管理工作的新方法.     

板股韧带的MRI研究及临床意义

目的　明确板股韧带正常MRI表现 ,建立由它所致假撕裂与外侧半月板后角真撕裂的鉴别方法。资料与方法　采用 6 0侧正常膝进行矢状和冠状位MR扫描 ,观察板股韧带的MRI表现。结果　板股韧带显示率为88.3% (5 3侧 ) ,其中板股前韧带为 2 6 .7% (16侧 )、板股后韧带为 86 .7% (5 2侧 )和两条韧带同时存在为 2 3.3% (14侧 )。板股韧带在矢状像上表现为位于后交叉韧带前或后方的类圆形或短棒状低信号结构 ,而在冠状像上表现为自外侧半月板后角至股骨内侧髁外侧面的条带样低信号结构。在矢状像上 ,5 2 .8% (2 8/5 3)板股韧带与外侧半月板后角之间显示出一线样高信号 ,被称为假撕裂 ,易与外侧半月板后角撕裂混淆。但假撕裂仅有两种走行方向即后下斜行 (2 1/2 8)或垂直方向 (7/2 8)。结论　根据假撕裂位置、方向以及冠状像和连续矢状面的观察 ,可正确区分外侧半月板后角真假撕裂     

夜间电子生物阻抗测量装置对阴茎勃起功能障碍的检测

目的 :探讨夜间电子生物阻抗测量装置 (NEVA)在阴茎勃起功能障碍的诊断中的应用价值。方法 :对 4 1例主诉阴茎勃起功能障碍者 (障碍组 ) 19例主诉无勃起功能障碍但有射精障碍者 (无障碍组 )进行NEVA检测。结果 :障碍组夜间试验中的夜间勃起的次数、阴茎勃起的最大体积改变、最长持续时间等客观指标均要差于无障碍组 ,差异有统计学意义 (P <0 .0 1)。结论 :NEVA为非侵入性检测、可鉴别心理性阴茎勃起功能障碍和严重的器质性勃起功能障碍 ,且对勃起功能障碍的定性和定量具有一定的客观性。     

聚氨酯避孕套临床前使用可行性和可接受性分析

目的 对使用新型聚氨酯(Pu)男用避孕套的可行性和可接受性进行初步观察。方法 对30对健康已婚夫妇为期6周360次使用国产PU避孕套的效果进行问卷调查。结果 避孕套总破裂率(临床破裂 非临床破裂)为2．22％，临床滑脱率为16．5％，使用观察期间无意外妊娠发生。结论 新型PU避孕套的破裂率和滑脱率分别相近于和高于报道的乳胶避孕套使用情况，感官指标反映PU避孕套具有良好的可接受性。     

隐性脊柱裂排尿功能异常的临床特征

目的：研究伴有排尿功能障碍的隐性脊柱裂患者临床特征。方法：总结41例有明显排尿功能障碍的隐性脊柱裂患者临床资料。以18岁为界，将患者分为儿童组和成人组，所有患者均接受X线检查，除4例儿童外，均接受普通尿流动力学检查。结果：儿童组发病高峰年龄在1～2岁，主要临床表现为持续性原发性遗尿，部分伴有尿频、尿急及轻度急迫性尿失禁现象；成人组发病高峰年龄在18～26岁，主要临床表现为尿频、尿急、排尿前踌躇、排尿困难、遗尿等。结论：隐性脊柱裂患者常有排尿功能异常表现，出现症状的高峰时间为出生后及青春发育期后。X线、CT、MRI和尿流动力学等检查在隐性脊柱裂排尿功能障碍的诊断和治疗方案制定中有重要地位。     

正畸固定矫治前后牙齿冷光美白疗效的对比研究

目的:了解经固定矫治的正畸患者在牙齿美白治疗后牙齿表面中部出现较规则斑块的成因。方法:分别对正畸及非正畸治疗患者、正畸治疗前后拔除的离体牙进行牙齿美白治疗,对牙列组进行视觉对比,对离体牙组进行扫描电镜对比。结果:经正畸治疗的牙列在美白治疗后牙面有色斑存在,而正畸治疗后拔除的离体牙的扫描电镜结果则显示有釉质-树脂突嵌合层存在。结论:经正畸治疗后牙齿表层的釉质-树脂突嵌合层是导致牙面出现色斑的原因。