DNA酸解后甲基绿——派洛宁改良染色法在胃癌,食管癌诊断中的应用

目的 对ＤＮＡ酸解后甲基酸－－派洛宁染色进行改良并用于肿瘤诊断。方法 收集已明确诊断的常规外检标本胃癌及食管癌共１２６例，应用改良染色法对ＤＮＡ酸解后组织进行染色观察。结果 被染组织色彩艳丽可清楚显示肿瘤细胞的染色强度及染色数量，清楚显示肥大细胞的形态特点，也显示其他多种组织成分。结论 ＤＮＡ酸解后甲基绿－－派洛宁改良染色法可用于判定肿瘤的增殖活性及分化程度，用于区别良、恶性病变，也适用于其他病理外检的诊断及组织形态学研究。     

参芪扶正注射液联合化疗治疗恶性肿瘤的临床研究

目的比较参芪扶正注射液联合化疗与单纯化疗治疗恶性肿瘤的临床疗效及不良反应。方法入选的62例患者随机分为两组,观察组32例,采用参芪扶正联合化疗,平均化疗5.7周期。对照组30例,单纯化疗,平均化疗5.6周期。结果观察组完全缓解（CR）3例,部分缓解（PR）12例,稳定（NC）11例,进展（PD）6例,总有效率46.9%。对照组CR 1例,PR 9例,NC 11例,PD 9例,总有效率33.3%。治疗组与对照组比较,治疗组T细胞亚群CD3、CD4和CD4/CD8比值明显升高,差异有显著性（P〈0.01）。治疗组生活质量较对照组明显改善。结论参芪扶正注射液联合化疗治疗恶性肿瘤可以减轻化疗的不良反应,使患者化疗依从性更好,增强机体的免疫功能,改善患者生活质量。     

阿司匹林与可待因的协同镇痛作用研究

通过小鼠热板致痛试验模型、小鼠醋酸致扭体反应试验模型以及大鼠光热辐射所致痛反应试验模型来研究阿司匹林和可待因的协同镇痛作用.结果表明,两药同用可以使镇痛作用加强,镇痛E琏0减小,表明两者的镇痛作用协同.     

迅速达到稳态浓度的三次相继输注法

本文通过研究三次相继输注时血药浓度的情况,提出了一种新的给药方案,即以速率Q_1输注T_1h,然后以速率Q_2输注T_2-T_1h,最后以维持速率Q_3输注到结束。文中采用优化手段得到三个输注速率的关系,从而使血药浓度尽快地达到稳态,并能控制血药浓度的最大值及其在指定时间内到达所期望的浓度。     

葛根素缓释复合骨架片理化性质的研究

目的利用甲壳胺 海藻酸钠的聚合物作为新型缓控释制剂的复合骨架材料 ,并对其性质及影响因素进行研究。方法利用天然高分子材料甲壳胺 (CS) (阳离子型 )与海藻酸钠 (AL) (阴离子型 )模拟在体内条件下形成聚电解质复合物。通过DTA及IR图谱的峰值变化证明这种复合物确实存在 ,形成的关键是控制好两种电解质的比例。结果当WCS∶WAL=2∶3时 ,反应最完全。利用制成的聚电解质复合物 ,制备葛根素缓释复合骨架片 ,缓释效果符合中国药典规定。结论甲壳胺 海藻酸钠形成的复合物可以做缓、控释制剂的骨架材料。     

Analysis of Pyrethroid Insecticides in <Emphasis Type="Italic">Chironomus dilutus</Emphasis> Using Matrix Solid Phase Dispersion Extraction

A matrix solid phase dispersion method was developed to detect eight pyrethroid insecticides in the aquatic invertebrate, Chironomus dilutus. A mixture of silica gel, diatomaceous earth and primary/secondary amino solid absorbents were selected as the dispersion matrix, while 7% ethyl ether in hexane was used as the elution solvent. Method detection limits for the target pyrethroids ranged from 0.46 to 4.4 μg kg⁻¹, and recoveries were 63.5%–124.0%, 43.7%–116.0% and 53.1%–93.1% at spiked levels of 5, 20 and 50 μg kg⁻¹, respectively. The developed method was used to assess pyrethroid residues in laboratory-exposed and field-collected C. dilutus.     

如皋市甲型肝炎疫苗免疫接种成本效益分析

采用ＷＨＯ推荐的“国民收入法”评价指标，分析研究推广接种甲肝疫苗预防和控制甲肝的成本效益。１９９５～１９９９年如皋市实施甲肝疫苗接种，甲型病毒性肝炎发病率明显下降，测算减少甲型肝炎病人１０７７４例，取得的经济效益为４２４８．９７万元，去除成本后的净效益为３７７９．４６万元，成本效益比为１：９．０５。结果表明，接种甲肝疫苗其经济效益显。     

Effect of fructose or sucrose feeding with different levels on oral glucose tolerance test in normal and type 2 diabetic rats

This study was designed to determine whether acute fructose or sucrose administration at different levels (0.05 g/kg, 0.1 g/kg or 0.4 g/kg body weight) might affect oral glucose tolerance test (OGTT) in normal and type 2 diabetic rats. In OGTT, there were no significant differences in glucose responses between acute fructose- and sucrose-administered groups. However, in normal rats, the AUCs of the blood glucose response for the fructose-administered groups tended to be lower than those of the control and sucrose-administered groups. The AUCs of the lower levels fructoseor sucrose-administered groups tended to be smaller than those of higher levels fructose- or sucrose-administered groups. In type 2 diabetic rats, only the AUC of the lowest level of fructose-administered (0.05 g/kg body weight) group was slightly smaller than that of the control group. The AUCs of fructose-administered groups tended to be smaller than those of the sucrose-administered groups, and the AUCs of lower levels fructose-administered groups tended to be smaller than those fed higher levels of fructose. We concluded from this experiment that fructose has tendency to be more effective in blood glucose regulation than sucrose, and moreover, that smaller amount of fructose is preferred to larger amount. Specifically, our experiments indicated that the fructose level of 0.05 g/kg body weight as dietary supplement was the most effective amount for blood glucose regulation from the pool of 0.05 g/kg, 0.1 g/kg and 0.4 g/kg body weights. Therefore, our results suggest the use of fructose as the substitute sweetener for sucrose, which may be beneficial for blood glucose regulation.     

Protective effects of sodium para‐amino salicylate on manganese‐induced neuronal death: the involvement of reactive oxygen species

Objectives This study tested whether sodium para‐amino salicylic dihydrate, an antibacterial drug for tuberculosis, could block manganese‐induced apoptosis in SK‐N‐MC neurons. Methods Cell viability, Hoechst staining, dichlorofluorescin diacetate analysis for reactive oxygen species measurement, and immunoblotting were performed. Key findings In vitro, manganese chloride significantly decreased the viability of SK‐N‐MC cells, accompanied by apoptotic features such as changes in nuclear morphology. Sodium para‐amino salicylic dihydrate inhibited these apoptotic characteristics through reducing intracellular reactive oxygen species generation, protecting mitochondrial membrane potential and caspase‐3 activation. Conclusions Sodium para‐amino salicylic dihydrate inhibits manganese‐induced apoptosis in neurons and may reduce manganese‐mediated neurodegeneration.