Selection of continence products: perspectives of people who have incontinence and their carers

Purpose : The needs, issues and concerns of people with incontinence and their carers were explored in order to inform the development of a comprehensive Australian consumer guide to continence products.

Participants and methods : Semi-structured interviews and focus groups were conducted with a wide range of people with incontinence and carers. Questions centred around issues and concerns regarding continence product selection, what information people need to make informed choices, and how the information should be presented. Qualitative analysis highlighted common themes and specific issues.

Results : Issues raised by consumers included the paucity of written information currently available, ignorance among health professionals concerning continence products and the lack of information about sources of help. Most consumers selected products by trial and error and were unaware of the range of products and services available. They wanted a consumer guide to continence products to be user-friendly and easily accessible, with information on product availability, cost, quality, comfort and design. They also wanted more general information on incontinence, sources of help, and how to select, care for and dispose of products. Participants wanted the guide to be made available in different languages, in large print and simple language.

Conclusions : The findings highlight the need and provide a framework for a consumer guide and information on continence products.     

Intravital imaging of the kidney using multiparameter multiphoton microscopy

Intravital optical microscopy provides a powerful means of studying the cell biology in the most physiologically relevant setting. The ability of multiphoton microscopy to collect optical sections deep into biological tissues has opened up the field of intravital microscopy to high-resolution studies of multiple organs. Presented here are examples of how two-photon microscopy can be applied to intravital studies of kidney physiology and the study of disease processes. These include studies of cell vitality and apoptosis, fluid transport, receptor-mediated endocytosis, blood flow, and leukocyte trafficking. Efficient two-photon excitation of multiple fluorophores permits comparison of multiple probes and simultaneous characterization of multiple parameters. Two-photon microscopy can now provide a level of investigation previously unattainable in intravital microscopy, enabling kinetic analyses and physiological studies of the organs of living animals with subcellular resolution. Therefore, application of this technology will provide direct visualization of organ-specific and cell-specific responses to an array of stimuli and therapeutic approaches, enhancing our understanding and treatment of disease processes.     

A phthalide with in vitro growth inhibitory activity from an oidiodendron strain

A screening campaign was implemented utilizing capillary electrophoresis as a primary assay to discover binders to the cancer target Akt1 from a crude natural extract library. Fungal extracts with binding activities were characterized for biochemical inhibition of Akt1 to phosphorylate the downstream substrate protein Bad. One of the crude extracts with bioactivity selected for isolation and structure elucidation from fermentation of the fungal culture Oidiodendron sp. F01895 yielded a new trihydroxy phthalide (1). The structure of 1 was determined by a combination of 1D and 2D NMR spectroscopic data along with high-resolution mass spectrometric data. Compound 1 displays inhibition of Akt1 biochemical activity in vitro and confers growth inhibition on some cancer-derived cell lines in culture.     

State substance abuse treatment gaps

This study estimated the adequacy of state substance abuse treatment rates relative to treatment needs. The investigators created composite drug and alcohol treatment need indexes from explicit-mention mortality and substance-defined arrest rates. The indexes were reliable and had evidence of construct validity, but alternative population-at-risk and survey-based need measures did not fair as well. States varied substantially in per capita alcohol and drug treatment needs, although the two did not correlate with each other. While the need indexes correlated significantly with state treatment rates, the adequacy of state treatment rates varied greatly. States with the largest treatment gaps were in the South, Southwest, and northern plains and mountain regions. The failure of the Block Grant formula to reflect the needs of rural states with high-risk minority populations may contribute to disparities in access to services.     

Excitotoxic lesions of the medial prefrontal cortex attenuate fear responses in the elevated-plus maze,social interaction and shock probe burying tests

Previous research investigating the effects of medial prefrontal cortex (MPFC) lesions on fear- and anxiety-related behavior has yielded an inconsistent body of findings. Behavioral studies have reported increases, decreases, and no effect on anxiety. In addition, many studies are complicated by the use of lesioning techniques that destroy fibers of passage, and the use of conditioned fear tests, which may introduce the confounding effects of learning and memory. Therefore, the present study examined the effects of ibotenic acid lesions of the MPFC (including prelimbic, infralimbic and anterior cingulate) on three wide-ranging and well-validated behavioral assays of anxiety: the elevated plus maze (EPM), social interaction (SI) and the shock-probe tests (SP). In the EPM test, lesioned rats showed a significantly higher percentage of open arm entries and open arm time than controls. In a version of the SI test sensitive to anxiolytic effects, lesioned rats were found to spend a significantly greater amount of time in active interaction with a conspecific; while another version of the SI test sensitive to anxiogenic effects did not show any differences between lesioned and non-lesioned controls. In the SP test, lesioned rats exhibited significantly lower rates of burying. In contrast, retention of shock probe avoidance was not affected. No effects of lesions on measures of locomotor activity or shock reactivity were found. The concordant anxiolytic-like effects found in the three behavioral assays strongly suggests a general reduction in fear responsiveness in MPFC lesioned rats.     

Near-infrared imaging in the small animal brain: optimization of fiber positions

We investigate fiber placement issues associated with a hybrid magnetic resonance imaging (MRI) near-infrared (NIR) imaging technique for small animal brain studies. Location of the optical fibers on the cranium is examined, with an emphasis on maximizing the recovered resolution and contrast in the region of interest, which in this case is the murine brain. In a series of simulation studies, singular value decomposition of the Jacobian is used in order to determine the measurement sites that provide the most information about the region of interest. The modeling results indicate that data collected using fibers arranged on one side of the head near the brain contain as much information about optical changes within the brain as those positioned equally spaced around the entire periphery of the head. Practical space limitation considerations favor the one-sided fiber array geometry in the case where the NIR acquisition is expected to occur simultaneously with MRI.