高效液相色谱法测定槐榆丸中芦丁含量的研究汉

目的 建立以高效液相色谱(HPLC)法快速测定槐榆丸中芦丁含量的方法.方法 色谱柱为Kromasil-C18流动相为甲醇-1%冰醋酸溶液(42∶58),检测波长为358 nm,流速为1.0 ml/min.结果 芦丁线性范围为115.2～1 152 ng(r=0.999 9),平均加样回收率为98.16%(RSD=0.54%).结论 方法简便、快速、分离度好、结果稳定,可用于槐榆丸的质量控制.     

肾衰泄浊颗粒主要药效学及急性毒性实验研究

目的:观察肾衰泄浊颗粒对慢性肾功能衰竭(CRF)大鼠的影响并观察其急性毒性反应。方法:建立CRF大鼠模型,观察CRF大鼠使用肾衰泄浊颗粒前后肾功能及血液学指标的变化情况。将肾衰泄浊颗粒以最大浓度给小鼠灌胃,小鼠没有出现死亡,未测出LD50故测其最大耐受量。结果:肾衰泄浊颗粒能明显降低腺嘌呤所致CRF大鼠的血肌酐(Scr)、尿素氮(BUN)水平,能提高红细胞(RBC)、血红蛋白(HGB)和红细胞压积(HCT)。肾衰泄浊颗粒给小鼠灌服的最大耐受量可达46.16g/kg,相当于临床用量的276.96倍。结论:肾衰泄浊颗粒是治疗CRF大鼠的有效药物,且具有较好的安全性。     

复方甘草甜素联合西替利嗪及多虑平治疗荨麻疹

目的:分析复方甘草甜素片联合西替利嗪及多虑平治疗慢性荨麻疹的疗效和可行性.方法:将296例慢性荨麻疹患者随机分为两组,对照组144例,西替利嗪10 mg,p0.qd,多虑平25 mg,po tid;治疗组152例,在对照组的基础上加服复方甘草甜素片75 mg,tid,两组均连用4周.结果:治疗组和对照组有效率分别为82.89%、61.11%,两组比较差异有统计学意义(P<0.01).结论:复方甘草甜素片联合西替利嗪及多虑平治疗慢性荨麻疹的疗效满意,值得临床选择应用.     

两种保守性手术治疗输卵管妊娠临床分析

目的:比较经腹腔镜与开腹保守性手术治疗输卵管妊娠的临床效果。方法:将146例确诊为输卵管妊娠、包块直径≤5cm且有生育要求的患者,随机分为腹腔镜组76例和开腹组70例,比较两组保守性手术手术时间、住院时间、术中出血量、手术成功率及术后血β-hCG恢复情况。结果:与开腹组相比,腹腔镜组手术成功率高(96.05%∶81.49%,P<0.05),术中出血少〔(32.7±14.3)ml∶(81.6±41.2)ml,P<0.05〕,手术时间短〔(41±19)min∶(57±12)min,P<0.05〕,住院时间短〔(4.9±0.8)天∶(7.0±2.3)天,P<0.05〕,肛门排气时间短〔(17.1±5.6)h∶(32.0±8.4)h,P<0.05〕,手术后血β-hCG恢复正常时间两组比较无显著差异(P>0.05)。结论:腹腔镜保守治疗输卵管妊娠具有手术时间短、术中出血少、术后患者肠道功能恢复迅速、住院时间短等优点,且手术成功率高,值得推广应用。     

基于Web和SQL／ASP技术构建胆石病数据挖掘系统

目的探讨基于Web和SQL/ASP技术、采用B/S模式构建胆石病数据查询和挖掘系统的方法,验证胆石病中医病因病机诊断标准和结果的准确性.方法以胆石病患者的临床病案资料为基础,构建胆石病数据查询和挖掘系统,同时运用SQL Server 2005建立胆石病中医病因病机Logistic Regression模型,对胆石病临床症状进行分析,预测中医病因病机的分布.结果建立了基于Web & SQL/ASP技术、采用B/S模式结构的胆石病数据查询和挖掘系统,设计了胆石病中医病因病机Logistic Regression模型.结论基于Web和SQL/ASP技术设计的医用信息系统,不仅能高效地管理和利用医院各类信息资料,便于归档和查询,同时通过对不断累积的数据进行挖掘,能为理论研究和临床实践提供更精准的信息支持.     

Evidence for a NOD2-independent susceptibility locus for inflammatory bowel disease on chromosome 16p

Heritable predisposition to inflammatory bowel disease (IBD) has been demonstrated by epidemiological and genetic analysis. Linkage of IBD to broad regions of chromosome 16 has been established by analysis of multiple populations. NOD2, located on proximal 16q, was recently identified as an IBD gene. As the linkage regions on chromosome 16 are large, we have investigated the possibility that NOD2 is not the only IBD gene located on this chromosome. A high-density experiment using 39 microsatellite markers was performed to identify additional regions of association, and to indicate areas of interest for further investigation. A triple-peaked configuration of the linkage curve with peak logarithm of odds (lod) scores of 2.7, 3.2, and 3.1 was observed on proximal 16p, proximal 16q, and central 16q, respectively. The cohort was stratified by coding individuals carrying the NOD2 single nucleotide polymorphism (SNP)8 and SNP13 "unknown." Significance at the central peak, corresponding to the genomic location of NOD2, then decreased from 3.2 to 1.2. The maximal lod scores on the proximal p-arm (lod = 2.1) and central q-arm (lod = 2.6) changed only moderately. An exploratory association analysis (TRANSMIT) yielded a strong lead at D16S3068 (P = 0.00028). The region around this marker was further investigated by using anonymous SNPs. An associated haplotype containing three SNPs was identified (peak significance P = 0.00027, IBD phenotype). On stratification based on NOD2 genotype, this significance increased to P = 0.0001. These results confirm the importance of NOD2 and provide evidence for a second IBD gene located on chromosome 16p.     

Effects of cytotoxic hippocampal lesions in mice on a cognitive test battery

Mice received cytotoxic lesions which selectively removed all of the hippocampus and dentate gyrus except the most ventral portions. They were impaired on both spontaneous and rewarded discrete-trial alternation in T-mazes. Acquisition of reference memory for the location of a hidden platform in the Morris water maze was impaired in lesioned mice. On an elevated Y-maze reference memory task, in which only one arm was rewarded, lesioned mice showed no evidence of learning. In a Lashley III maze task, however, where maze rotation demonstrated that control performance was independent of distal spatial cues, acquisition in the lesioned mice was unimpaired. Control levels of continuous spontaneous alternation in a Y-maze were too low to reveal a hippocampal deficit. A small impairment in acquisition of a multiple-trial passive avoidance task was seen in lesioned mice, despite a small but significant increase in reactivity to the footshock. These results are largely consistent with findings in hippocampal lesioned rats on the same or similar tasks, and reflect a major impairment of spatial cognition, with relative sparing of non-spatial task performance.     

羊膜和角膜干细胞移植治疗眼表疾病临床观察

目的探讨羊膜移植、自体角膜干细胞移植及自体体外培养角膜干细胞移植重建眼表治疗眼表疾病的疗效。方法眼表疾病43例46眼（瘢痕性结膜囊狭窄、睑球粘连6例6眼，角结膜化学、热烧伤10例13眼，原发和复发性翼状胬肉切除术后结膜缺损27例27眼）进行羊膜移植、自体角膜干细胞移植及自体体外培养角膜干细胞移植，观察植片及角结膜上皮愈合、组织浸润和新生血管情况。结果羊膜移植术重建结膜囊效果满意，睑球粘连松解。羊膜、自体体外培养角膜干细胞移植术治疗角结膜烧伤均可使眼表上皮化，基质炎性浸润减轻，新生血管明显减少；自体体外培养角膜干细胞移植对角膜缘干细胞损伤严重的患者效果显著。自体和自体体外培养角膜干细胞移植治疗翼状胬肉均有良好效果，术后不适症状轻时间短，角膜创面愈合快，随访（1～2）y两种术式各复发1眼。自体体外培养角膜干细胞移植对复发性翼状胬肉切除术后较大的结膜缺损更为适合。结论羊膜移植、自体角膜干细胞移植和自体体外培养角膜干细胞移植对重建眼表都有重要的功效，手术方式的选择必须根据角膜干细胞损伤的程度、范围进行选择。     

胃肠道无线内窥镜中可吞服摄像药丸的研究

介绍无线摄像药丸直径11mm,长30 mm,包括CMOS图像传感器、光学系统、电池、发射电路及天线等.利用MEMS工艺将CMOS图像传感器和驱动电路微型化,也可将发射电路集成化.图像信号有模拟和数字两种传输方式.数字方式使用串行数据接口传输图像.最后介绍药丸外壳的加工工艺.