全文获取类型
收费全文 | 2156篇 |
免费 | 85篇 |
国内免费 | 30篇 |
专业分类
耳鼻咽喉 | 3篇 |
儿科学 | 95篇 |
妇产科学 | 19篇 |
基础医学 | 418篇 |
口腔科学 | 77篇 |
临床医学 | 248篇 |
内科学 | 493篇 |
皮肤病学 | 112篇 |
神经病学 | 39篇 |
特种医学 | 266篇 |
外科学 | 175篇 |
综合类 | 48篇 |
预防医学 | 73篇 |
眼科学 | 25篇 |
药学 | 138篇 |
中国医学 | 1篇 |
肿瘤学 | 41篇 |
出版年
2015年 | 24篇 |
2014年 | 19篇 |
2013年 | 74篇 |
2012年 | 23篇 |
2010年 | 44篇 |
2009年 | 30篇 |
2008年 | 22篇 |
2007年 | 42篇 |
2006年 | 44篇 |
2005年 | 24篇 |
2004年 | 18篇 |
2003年 | 23篇 |
2001年 | 29篇 |
2000年 | 23篇 |
1999年 | 35篇 |
1998年 | 82篇 |
1997年 | 95篇 |
1996年 | 104篇 |
1995年 | 70篇 |
1994年 | 73篇 |
1993年 | 76篇 |
1992年 | 39篇 |
1991年 | 36篇 |
1990年 | 41篇 |
1989年 | 66篇 |
1988年 | 57篇 |
1987年 | 48篇 |
1986年 | 51篇 |
1985年 | 38篇 |
1984年 | 55篇 |
1983年 | 27篇 |
1982年 | 31篇 |
1981年 | 37篇 |
1980年 | 49篇 |
1979年 | 23篇 |
1978年 | 27篇 |
1977年 | 35篇 |
1976年 | 38篇 |
1975年 | 36篇 |
1972年 | 24篇 |
1971年 | 21篇 |
1970年 | 23篇 |
1969年 | 18篇 |
1965年 | 50篇 |
1964年 | 50篇 |
1963年 | 47篇 |
1962年 | 41篇 |
1961年 | 40篇 |
1960年 | 65篇 |
1959年 | 20篇 |
排序方式: 共有2271条查询结果,搜索用时 15 毫秒
71.
E García‐Molina J Lacunza F Ruiz‐Espejo M Sabater A García‐Alberola JR Gimeno F Caizares A García P Martínez M Valds I Tovar 《Clinical genetics》2013,83(6):530-538
We aim to study the SCN5A gene in a cohort of Brugada syndrome (BS) patients and evaluate the genotype–phenotype correlation. BS is caused by mutations in up to 10 different genes, SCN5A being the most frequently involved. Large genomic rearrangements in SCN5A have been associated with conduction disease, but its prevalence in BS is unknown. Seventy‐six non‐related patients with BS were studied. Clinical characteristics and family risk profile were recorded. Direct sequencing and multiplex ligation‐dependent probe amplification (MLPA) of the SCN5A gene for identification of mutations and larger rearrangements were performed, respectively. Eight patients (10.5%) had point mutations (R27H, E901K, G1743R (detected in three families), V728I, N1443S and E1152X). Patients with mutations had a trend toward a higher proportion of spontaneous type I Brugada electrocardiogram (ECG) (87.5% vs 52.9%, p = 0.06) and had evidence of familial disease (62.5%, vs 23.5%, p = 0.03). The symptoms and risk profile of the carriers were not different from wild‐type probands. There were non‐significant differences in the prevalence of type I ECG, syncope and history of arrhythmia in carriers of selected polymorphisms. None of the patients had any deletion/duplication in the SCN5A gene. In conclusion, 10.5% of our patients had mutations in the SCN5A gene. Patients with mutations seemed to have more spontaneous type I ECG, but no differences in syncope or arrhythmic events compared with patients without mutations. Larger studies are needed to evaluate the role of polymorphisms in the SCN5A in the expression of the phenotype and prognosis. Large rearrangements were not identified in the SCN5A gene using the MLPA technique. 相似文献
72.
73.
William M. Mihalko Anita L. Kerkhof Marcus C. Ford John R. Crockarell James W. Harkess James L. Guyton 《The Journal of arthroplasty》2021,36(5):1590-1598
BackgroundWe hypothesized that preoperative cryoneurolysis of the superficial genicular nerves in patients with osteoarthritis would decrease postoperative opioid use relative to standard of care (SOC) treatment in patients undergoing total knee arthroplasty (TKA).MethodsPatients received either cryoneurolysis (intent-to-treat [ITT]: n = 62) or SOC (ITT: n = 62). The cryoneurolysis group received cryoneurolysis of the superficial genicular nerves 3–7 days before surgery plus a similar preoperative, intraoperative, and postoperative pain management protocol as the SOC group. The primary end point was cumulative opioid consumption in total daily morphine equivalents from discharge to the 6-week study follow-up assessment. Secondary end points included changes in pain and functional scores. Primary and secondary end points were assessed using ITT and per-protocol (PP) analyses.ResultsThe primary end point was not met in the ITT analysis (4.8 [cryoneurolysis] vs 6.1 [SOC] mg; P = .0841) but was met in the PP analysis (4.2 vs 5.9 mg; P = .0186) after excluding patients with medication deviations or missing follow-up data. Compared with the SOC group, the cryoneurolysis group had improved functional scores and numerical improvements in pain scores across all follow-up assessments, with significant improvements observed in current pain from baseline to the 72-hour and 2-week follow-up assessments and pain in the past week from baseline to the 12-week follow-up assessment.ConclusionFindings from the PP analysis suggest that preoperative cryoneurolysis in patients with knee osteoarthritis can reduce opioid consumption and improve functional outcomes after TKA. 相似文献
74.
75.
76.
77.
78.
1. Three cases of hereditary hemolytic disease secondary to G-6-PD deficiency are described. Two of the cases were first cousins of Scotch-Irish-English descent and the mode of inheritance was believed to be sex-linked.The third case was of Turkish origin; no family studies were availale.2. The mothers, who were heterozygous for G-6-PD deficiency, showed onlyminimal expression of the defect, which was manifested by a slightly decreasedred cell survival in both mothers and an abnormal methemoglobin reductiontest in one of them.3. All three cases showed a more pronounced fall in erythrocyte ATP afterincubation with phenylhydrazine than that observed in primaquine-sensitiveNegroes whose red cells were less deficient in G-6-PD.4. It is suggested that the inability of the G-6-PD-deficient erythrocyte tomaintain adequate levels of ATP may be an important factor in the pathogenesis of the hemolytic process. Submitted on August 26, 1963 Accepted on October 24, 1963 相似文献
79.
Interventions in saphenous vein grafts present some of the most challenging problems in preventing acute complications and limiting restenosis. Available options include repeat bypass surgery, balloon angioplasty, directional atherectomy, transluminal extraction atherectomy, rotational atherectomy, laser angioplasty, and stenting. Stenting appears to provide the best acute and long-term results. Debulking with directional atherectomy prior to stenting may be helpful but its role is unproven. With any device, it is essential to attain the lowest possible residual stenosis with the least amount of manipulation. Complications with vein graft interventions are most commonly related to distal embolization, which occurs most frequently in older vein grafts with diffuse disease, large plaque volume or thrombus, or those with total occlusion. Use of thrombolytics, glycoprotein Ilb/IIIa receptor inhibitors, and thrombectomy devices may be helpful when thrombus is present. Calcium channel blockers may be beneficial when embolization of plaque debris results in slow flow or no-flow during interventions. 相似文献
80.
恰如其分的外周髓鞘形成取决于雪旺细胞增殖与分化进程间的平衡。丝氨酸/苏氨酸激酶(mTOR)整合多种环境因素,是细胞生长、代谢、发挥作用的中枢调节者。本文报道了一种mTOR的负性调节剂——结节性硬化复合体(TSC1),通过控制细胞增殖和髓鞘稳态,建立了雪旺细胞谱系进展和髓鞘形成的阶段依赖性程序。小鼠雪旺细胞祖细胞中TSC1的解离导致mTOR信号通路激活,继而导致雪旺细胞过量增殖,分化受阻,髓鞘形成减少。转录组分析显示,TSC1突变体中的mTOR活化使得polo样激酶(PLK)依赖性通路和细胞周期调节剂上调。弱化mTOR或者对PLK进行药理抑制部分挽救了因TSC1缺失导致的外周神经发育过程中的髓鞘形成减少。相较之下,成年小鼠成熟雪旺细胞中TSC1缺失可导致髓鞘的过度增殖和过度生长。本文的发现提示了TSC1-mTOR-PLK信号轴在控制雪旺细胞的发育过程中,从增殖到分化和髓鞘内稳态中起到的阶段特异性功能。 相似文献