Using Sibling Differences to Estimate Effects of Parenting on Adolescent Sexual Risk Behaviors

PURPOSE: To estimate effects of positive and involved parenting during mid-adolescence on sexual risk behaviors (frequency of intercourse, unprotected intercourse, and number of sexual partners) during late adolescence. Substantial literature suggests that supportive family contexts and parenting behaviors may discourage adolescents from engaging in early and risky sexual activities; yet methodological limitations hamper the conclusions regarding causality and directionality that can be drawn from much existing research. To address such limitations, the current study used a variety of increasingly conservative statistical modeling techniques to help control for unobserved heterogeneity and potential bias and hence to progress toward identifying causal relationships. METHODS: Drawing from a nationally representative longitudinal survey of adolescents (NLSY97; N = 4980), this study used ordinary least squares (OLS) regression models, lagged regression models, and family fixed-effects models to assess whether parental knowledge, parent negativity, and family activities during midadolescence predicted differences in late adolescent sexual risk behaviors. RESULTS: Even after controlling for unobserved heterogeneity across individuals and across families, parenting processes significantly predicted later adolescent sexual risk behaviors. Specifically, more regular family activities and less negative and hostile parenting during mid-adolescence predicted lower sexual risk behaviors during late adolescence. CONCLUSIONS: Results concerning the buffering effects of parenting on adolescent risk behaviors help to inform prevention and intervention efforts. Through the use of more rigorous statistical methodology and large representative samples of youth, this research provides an exemplar of how survey research can seek to move closer to understanding causal processes in the exceedingly complex systems of human development.     

Discovery of novel targets of quinoline drugs in the human purine binding proteome

The quinolines have been used in the treatment of malaria, arthritis, and lupus for many years, yet the precise mechanism of their action remains unclear. In this study, we used a functional proteomics approach that exploited the structural similarities between the quinoline compounds and the purine ring of ATP to identify quinoline-binding proteins. Several quinoline drugs were screened by displacement affinity chromatography against the purine binding proteome captured with gamma-phosphate-linked ATP-Sepharose. Screening of the human red blood cell purine binding proteome identified two human proteins, aldehyde dehydrogenase 1 (ALDH1) and quinone reductase 2 (QR2). In contrast, no proteins were detected upon screening of the Plasmodium falciparum purine binding proteome with the quinolines. In a complementary approach, we passed cell lysates from mice, red blood cells, or P. falciparum over hydroxychloroquine- or primaquine-Sepharose. Consistent with the displacement affinity chromatography screen, ALDH and QR2 were the only proteins recovered from mice and human red blood cell lysate and no proteins were recovered from P. falciparum. Furthermore, the activity of QR2 was potently inhibited by several of the quinolines in vitro. Our results show that ALDH1 and QR2 are selective targets of the quinolines and may provide new insights into the mechanism of action of these drugs.     

Dural arteriovenous fistulae: noninvasive diagnosis with dynamic MR digital subtraction angiography

MR digital subtraction angiography (DSA) is a new diagnostic tool capable of producing dynamic images of the cerebral circulation with the injection of gadopentetate dimeglumine into a peripheral vein. Previous reports have concentrated on its potential as a noninvasive technique for the study of pial arteriovenous malformations. In this report, we present our early findings with MR DSA in the evaluation of intracranial dural arteriovenous fistulae.     

Cocaine use in pregnancy in Amsterdam

To study the effects of cocaine use in pregnancy in Amsterdam, clinical data on cocaine-using pregnant women ( n = 21) and their offspring ( n = 23) were obtained retrospectively (1987–1994) at the Academic Medical Center, Amsterdam. Infants exposed to cocaine had a median gestational age of 39 weeks and a median birth weight of 3090 g. There were six preterm infants, two small-for-gestational-age infants and five infants with a small head circumference. Three infants had a congenital malformation. One infant (Potter's syndrome) died shortly before birth. One infant had congenital syphilis, four had intracerebral abnormalities on ultrasound and four had abnormal neurologic symptoms in the neonatal period. One infant died after 21 days of life. At follow-up four infants showed abnormal development. In 12 of the 23 infants (52%), one or more possible effects of cocaine were found.     

Building geographic information system capacity in local health departments: lessons from a North Carolina project

State government, university, and local health department (LHD) partners collaborated to build the geographic information system (GIS) capacity of 5 LHDs in North Carolina. Project elements included procuring hardware and software, conducting individualized and group training, developing data layers, guiding the project development process, coordinating participation in technical conferences, providing ongoing project consultation, and evaluating project milestones. The project provided health department personnel with the skills and resources required to use sophisticated information management systems, particularly those that address spatial dimensions of public health practice. This capacity-building project helped LHDs incorporate GIS technology into daily operations, resulting in improved time and cost efficiency. Keys to success included (1) methods training rooted in problems specific to the LHD, (2) required project identification by LHD staff with associated timelines for development, (3) ongoing technical support as staff returned to home offices after training, (4) subgrants to LHDs to ease hardware and software resource constraints, (5) networks of relationships among LHDs and other professional GIS users, and (6) senior LHD leadership who supported the professional development activities being undertaken by staff.