全文获取类型
收费全文 | 1082篇 |
免费 | 37篇 |
国内免费 | 147篇 |
专业分类
儿科学 | 57篇 |
妇产科学 | 5篇 |
基础医学 | 73篇 |
口腔科学 | 21篇 |
临床医学 | 166篇 |
内科学 | 263篇 |
皮肤病学 | 30篇 |
神经病学 | 27篇 |
特种医学 | 309篇 |
外科学 | 82篇 |
综合类 | 24篇 |
预防医学 | 38篇 |
眼科学 | 13篇 |
药学 | 111篇 |
1篇 | |
肿瘤学 | 46篇 |
出版年
2023年 | 3篇 |
2022年 | 4篇 |
2021年 | 7篇 |
2020年 | 7篇 |
2019年 | 12篇 |
2018年 | 14篇 |
2017年 | 7篇 |
2016年 | 11篇 |
2015年 | 12篇 |
2014年 | 19篇 |
2013年 | 38篇 |
2012年 | 13篇 |
2011年 | 20篇 |
2010年 | 35篇 |
2009年 | 44篇 |
2008年 | 27篇 |
2007年 | 107篇 |
2006年 | 22篇 |
2005年 | 29篇 |
2004年 | 9篇 |
2003年 | 14篇 |
2002年 | 20篇 |
2001年 | 19篇 |
2000年 | 13篇 |
1999年 | 18篇 |
1998年 | 78篇 |
1997年 | 77篇 |
1996年 | 72篇 |
1995年 | 62篇 |
1994年 | 42篇 |
1993年 | 51篇 |
1992年 | 8篇 |
1991年 | 16篇 |
1990年 | 12篇 |
1989年 | 49篇 |
1988年 | 31篇 |
1987年 | 31篇 |
1986年 | 25篇 |
1985年 | 26篇 |
1984年 | 20篇 |
1983年 | 12篇 |
1982年 | 27篇 |
1981年 | 14篇 |
1980年 | 14篇 |
1979年 | 6篇 |
1978年 | 4篇 |
1977年 | 17篇 |
1976年 | 29篇 |
1975年 | 17篇 |
1924年 | 1篇 |
排序方式: 共有1266条查询结果,搜索用时 0 毫秒
51.
52.
Muscle necrosis in the extremities: evaluation with Tc-99m pyrophosphate scanning--a retrospective review 总被引:1,自引:0,他引:1
A retrospective review was done of 34 extremities studied between 1981 and 1985 with technetium-99m pyrophosphate scanning; 22 were subsequently amputated. Results of detailed pathologic examination or immediate postoperative examination of the resected extremity were available in 16 cases. In these cases, scanning had allowed correct prediction of the level of amputation and of the specific areas of muscle infarction in 13 cases. In the one case in which amputation was performed for infection rather than muscle necrosis, the lack of necrosis was correctly predicted with the scan. The limited results of this study indicate that the Tc-99m pyrophosphate scan allows the location of necrotic muscle to be predicted accurately and may therefore be a useful adjunct in determining the best level for ultimate amputation. Special caution is required in those cases in which muscle necrosis is due to acute causes (e.g., traumatic thrombosis) rather than chronic vascular disease. 相似文献
53.
Expression and immunogenicity of oncofetal antigen-immature laminin receptor in human renal cell carcinoma 总被引:3,自引:0,他引:3
Zelle-Rieser C Barsoum AL Sallusto F Ramoner R Rohrer JW Höltl L Bartsch G Coggin JH JR Thurnher M 《The Journal of urology》2001,165(5):1705-1709
PURPOSE: The 32 to 44 kDa. oncofetal antigen-immature laminin receptor (OFA-iLR) is a multifunctional protein expressed by various tumors, including breast, lung, ovary and prostate carcinoma as well as lymphoma. OFA-iLR has been implicated in tumor invasiveness, metastasis and growth. Interferon-gamma producing effector T cells and interleukin (IL)-10 producing suppressor T cells specific for OFA-iLR have been described. MATERIALS AND METHODS: The 43515 IgG2a anti-OFA-iLR monoclonal antibody was used to detect OFA-iLR expression in human renal cell carcinoma tissue by flow cytometry and immunoblotting. Spontaneous or therapy induced immune responses against OFA-iLR were determined in patients with metastatic renal cell carcinoma. Proliferative and cytokine (interferon-gamma and IL-10) responses of peripheral blood mononuclear cells from patients with renal cell carcinoma against recombinant OFA-iLR were assessed. RESULTS: Using flow cytometry OFA-iLR was detected in all 13 tumors tested. Immunoblotting revealed differences in OFA-iLR expression in renal cell carcinoma and normal kidney tissue. OFA-iLR specific proliferative and cytokine responses of mononuclear cells were detected in all 6 patients tested. Importantly evidence was also obtained that treating metastatic renal cell carcinoma with tumor lysate pulsed dendritic cells would enhance OFA-iLR specific immunity. CONCLUSIONS: This study demonstrates that OFA-iLR is an immunogenic tumor associated antigen in human renal cell carcinoma. OFA-iLR specific effector T cells producing interferon-gamma may have a role in the control of tumor growth, whereas suppressor T cells producing IL-10 may promote tumor tolerance and, thus, tumor progression. 相似文献
54.
KH Neppelenbroek RS Seó VM Urban S Silva LN Dovigo JH Jorge NH Campanha 《Oral diseases》2014,20(4):329-344
In healthy individuals, Candida species are considered commensal yeasts of the oral cavity. However, these microorganisms can also act as opportunist pathogens, particularly the so‐called non‐albicans Candida species that are increasingly recognized as important agents of human infection. Several surveys have documented increased rates of C. glabrata, C. tropicalis, C. guilliermondii, C. dubliniensis, C. parapsilosis, and C. krusei in local and systemic fungal infections. Some of these species are resistant to antifungal agents. Consequently, rapid and correct identification of species can play an important role in the management of candidiasis. Conventional methods for identification of Candida species are based on morphological and physiological attributes. However, accurate identification of all isolates from clinical samples is often complex and time‐consuming. Hence, several manual and automated rapid commercial systems for identifying these organisms have been developed, some of which may have significant sensitivity issues. To overcome these limitations, newer molecular typing techniques have been developed that allow accurate and rapid identification of Candida species. This study reviewed the current state of identification methods for yeasts, particularly Candida species. 相似文献
55.
56.
Adverse reaction to intravenous gadoteridol 总被引:1,自引:0,他引:1
57.
58.
59.
60.
Immunohistochemical localization of membrane and alpha-granule proteins in human megakaryocytes: application to plastic-embedded bone marrow biopsy specimens 总被引:12,自引:2,他引:12
Using a new technique for antigen localization, we have demonstrated platelet proteins in megakaryocytes in plastic-embedded biopsy specimens of normal human bone marrow. In a series of 25 specimens, megakaryocytes showed labeling with antibodies to the integral membrane glycoproteins IIIa, IIb, and the IIb-IIIa complex; granule membrane protein 140; and five alpha-granule matrix proteins: thrombospondin, factor VIII-related antigen, beta-thromboglobulin, platelet factor 4, and fibrinogen. The antibodies to the membrane glycoproteins IIIa, IIb, and IIb-IIIa produced diffuse cytoplasmic staining and heavier staining on the plasma membrane, whereas the antibodies to the alpha-granule matrix proteins produced a distinct granular staining within the cytoplasm. Staining for granule membrane protein 140 was also granular in distribution. Rare mononuclear cells consistent with megakaryocyte precursors were labeled with these markers. Other enzyme histochemical and lectin-binding studies showed that the enzyme alpha-naphthyl acetate esterase, the lectin Ulex europaeus I, and the periodic-acid Schiff reaction were consistent, but not specific, markers of megakaryocytes. This immunohistochemical technique should facilitate the examination of qualitative and quantitative changes in megakaryocytes in a variety of physiologic and pathologic processes. 相似文献