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131.
Fluoxetine-induced change in rat brain expression of brain-derived neurotrophic factor varies depending on length of treatment 总被引:7,自引:0,他引:7
De Foubert G Carney SL Robinson CS Destexhe EJ Tomlinson R Hicks CA Murray TK Gaillard JP Deville C Xhenseval V Thomas CE O'Neill MJ Zetterström TS 《Neuroscience》2004,128(3):597-604
Recent studies indicate that brain-derived neurotrophic factor (BDNF) may be implicated in the clinical action of antidepressant drugs. Repeated (2-3 weeks) administration of antidepressant drugs increases BDNF gene expression. The onset of this response as well as concomitant effects on the corresponding BDNF protein is however, unclear. The present study investigated the effects of acute and chronic administration of the selective serotonin reuptake inhibitor, fluoxetine (10mg/kg p.o.), upon regional rat brain levels of BDNF mRNA and protein expression. To improve the clinical significance of the study, fluoxetine was administered orally and mRNA and protein levels were determined ex vivo using the techniques of in situ hybridisation histochemistry and immunocytochemistry respectively. Direct measurement of BDNF protein was also carried out using enzyme-linked immunosorbent assay (ELISA). Four days of once daily oral administration of fluoxetine induced decreases in BDNF mRNA (hippocampus, medial habenular and paraventricular thalamic nuclei). Whilst 7 days of treatment showed a non-significant increase in BDNF mRNA, there were marked and region-specific increases following 14 days of treatment. BDNF protein levels remained unaltered until 21 days of fluoxetine treatment, when the numbers of BDNF immunoreactive cells were increased, reaching significance in the pyramidal cell layer of CA1 and CA3 regions of Ammon's horn (CA1 and CA3) but not in the other sub-regions of the hippocampus. Indicative of the highly regional change within the hippocampus, the ELISA method failed to demonstrate significant up-regulation at 21 days, measuring levels of BDNF protein in the whole hippocampus. In contrast to the detected time dependent and biphasic response of the BDNF gene, activity-regulated, cytoskeletal-associated protein (Arc) mRNA showed a gradual increase during the 14-day course of treatment. The results presented here show that BDNF is expressed differentially depending on length of fluoxetine administration, which could contribute in explaining the slow onset of antidepressant activity observed with selective serotonin reuptake inhibitors. 相似文献
132.
Flow cytometry evaluation of cell-mediated cytotoxicity 总被引:1,自引:0,他引:1
D Zarcone A B Tilden G Cloud H M Friedman A Landay C E Grossi 《Journal of immunological methods》1986,94(1-2):247-255
A novel flow cytometry method for the evaluation of cell-mediated cytotoxicity is described. This method uses flow cytometry analysis to distinguish target cells from effector cells by differences in volume and light scatter characteristics. Non-viable target cells, following their interaction with effector cells, are determined via propidium iodide (PI) dye exclusion and then expressed as a percentage of the total target cell population. This assay is suitable both for analysis of systems which allow recycling of cytotoxic effector cells (total cell cytotoxicity assays, TCCA), and of systems in which recycling does not occur (single cell cytotoxicity assays, SCCA). Natural killer (NK) cell-mediated cytotoxicity evaluated by flow cytometry is significantly correlated with the standard 51Cr release assay. Flow cytometry can also be used to evaluate the competitive inhibition that certain cell types exert on the cell-mediated killing of NK-sensitive targets. A prerequisite for this assay is that competitor cells and target cells are distinguishable through their volume and light scatter characteristics. Advantages and pitfalls of the flow cytometry method are discussed, in comparison with the 51Cr-release assay. 相似文献
133.
Mirande Candito Christiane Soler Anne Deville Soumeya Bekri Pierre Chambon Antoine Thyss 《Pediatric blood & cancer》1996,26(1):57-60
Neuroblastomas are malignant tumors derived embryonically from the neural crest. Biological diagnosis relies on assay of urinary excretion of homovanillic acid (HVA), vanillylmandelic acid (VMA), and dopamine (DA). Spontaneous regression of these neoplasms has been reported by numerous investigators. The authors report the case of a child with neuroblastoma that illustrates the relationship between catecholamine metabolites and tumor maturation. At 1 month of age, this infant presented an adrenal neuroblastoma with multiple metastases (stage IV); the initial histological diagnosis based on examination of cutaneous metastases was neuroblastoma. At the age of 6 months, after chemotherapy, the primary tumor was resected; hepatic metastases were discovered at laparotomy. The histological diagnosis for all lesions was highly differentiated, mature ganglioneuroma-like tissue. The main biochemical abnormality at the time of diagnosis was an elevation in normetanephrine (NMN). HVA was only slightly increased but rose progressively during chemotherapy; it dropped back to normal levels after the sixth course. This case illustrates the potential benefits of separate assays of urinary methylated catecholamine metabolites for biochemical diagnosis and therapeutic management of neuroblastoma in addition to assays of HVA, VMA, and DA. Case findings suggest existence of a transformation process with maturation of the tumor involving enzymatic regulation and expression of MAO. © 1996 Wiley-Liss, Inc. 相似文献
134.
Endovascular treatment of a tuberculous infected aneurysm of the descending thoracic aorta: a word of caution 总被引:1,自引:0,他引:1
Labrousse L Montaudon M Le Guyader A Choukroun E Laurent F Deville C 《Journal of vascular surgery》2007,46(4):786-788
An infected aneurysm of the thoracic aorta due to mycobacterium tuberculosis is an unusual entity for which the classical treatment is antituberculosis chemotherapy and open-chest surgery. Recent improvements in endovascular treatments have led to their proposed use for infected aneurysms in patients for whom open surgery poses too high a risk. We report on a 68-year-old man with a tuberculous aortic aneurysm who had been treated with an endoprosthesis and antituberculosis chemotherapy. His clinical and radiological follow-up was uneventful and led to the discontinuation of pharmacological treatment after 16 months. However, a recurrence of the infection led to a fatal aortic rupture 4 months after discontinuation of therapy. 相似文献
135.
Neha R. Malhotra Jessica D. Smith Alexandra C. Jacobs Cali E. Johnson Uzer S. Khan Halle B. Ellison Benjamin J. Brintz Morgan M. Millar William G. Cloud Jeffry Nahmias Kimberly M. Hendershot Brigitte K. Smith 《American journal of surgery》2021,221(2):291-297
BackgroundThe ACGME mandates that residency programs provide training related to high value care (HVC). The purpose of this study was to explore HVC education in general surgery residency programs.MethodsAn electronic survey was distributed to general surgery residents in geographically diverse programs.ResultsThe response rate was 29% (181/619). Residents reported various HVC components in their curricula. Less than half felt HVC is very important for their future practice (44%) and only 15% felt confident they could lead a QI initiative in practice. Only 20% of residents reported participating in a root cause analysis and less than one-third of residents (30%) were frequently exposed to cost considerations.ConclusionFew residents feel prepared to lead quality improvement initiatives, have participated in patient safety processes, or are aware of patients’ costs of care. This underscores the need for improved scope and quality of HVC education and establishment of formal curricula. 相似文献
136.
The multitude of caval filters now available and conflicting experimental and clinical findings indicate that no one model can be considered to be perfect. The principal drawbacks of conical endocaval filters, such as the Greenfield model, are the possibility of migration and tilting, often related to failure to open, or untimely ejection of the filter. In this study, we analyzed the geometrical aspects of these filters, leaving aside all concerns of biocompatibility and thrombogenicity, as well as the hemodynamic or clinical aspects. The goal was to determine an ideal geometrical model, with optimal filtration of clots and low risk of tilting. 相似文献
137.
A retrospective analysis of primary eye cancer at the University of Alabama 1958-1988. Part 2: Eyelid tumors 总被引:2,自引:0,他引:2
Records of 117 patients with eyelid tumors on file at the University of Alabama at Birmingham were reviewed. Basal cell carcinoma was the single most common tumor, followed by squamous cell carcinoma, cutaneous melanoma, meibomian gland carcinoma and sebaceous gland carcinoma. A high percentage of patients with eyelid tumors were found to have secondary skin and non-skin tumors (41 percent). 相似文献
138.
139.
Cerebral mycotic or infective aneurysms are a rare complication of infectious illness, and such aneurysms of the intracavernous portion of the internal carotid artery are rare. They have been described as a consequence of cavernous sinus infection in an immunocompromised host, but not previously in a renal transplant recipient. We present such a case with serial MRI showing progression from arterial narrowing to aneurysm formation. Transcranial Doppler sonography of the middle cerebral artery showed distal asymptomatic embolisation from the aneurysm. 相似文献
140.