硫酸多糖对体外人脐静脉内皮细胞损伤的保护作用

研究表明,硫酸多糖体外对多聚阳离子和氧自由基损伤的人脐静脉内皮细胞有保护作用。肝素、硫酸软骨素A抗多聚阳离子损伤作用比同浓度低分子肝素和甘糖酯强。肝素、硫酸软骨素A、甘糖酯抗氧自由基损伤作用优于同浓度低分子肝素。结果显示硫酸多糖有保护血管内皮的作用,其作用可能与所带阴离子基团有关。     

泌尿系感染者病原菌及耐药性分析

目的：了解近年来泌尿系细菌感染致病菌及耐药性，为合理应用抗生素，提供临床根据。方法：对泌尿系感染患者298株病原菌进行药物敏感试验。结果：大肠埃希菌仍是引发泌尿系感染的主要病原菌，占53％，其次是葡萄球菌属，占19．40％，肠杆菌属占10．06％，肠球菌属占4．70％等，各类菌属耐药情况。结论：临床医师必须关注本地病原菌分布及耐药情况，合理应用抗生素，减少耐药性播散。     

心血管病流行病学研究必需的基础和专业知识

本文从心血管病流行病学研究工作的实际体会中提出:当前要提高科研工作水平的关键是提高研究防治队伍的科学素质.其中包括:应具备流行病学和生物统计学的基本知识,必要的心血管病临床知识和实践经验,关心常见心血管病的基础研究进展,和学习有关专业的知识,如营养学、行为医学、遗传学等.解决的办法除集中培训,个人努力学习外,更需组织有不同专业基础而有志于心血管病流行病学研究工作的人员密切合作,相互学习,取长补短,以达到共同提高.     

气功对冠心病人CM_5ST段/心率斜率的影响

37名冠心病患者随机分为气功治疗组(22人)和对照组(15人)。气功治疗组接受12周动功治疗,对照组接受安慰剂治疗,治疗前后均测定CM_5 ST段/心率斜率。结果发现动功治疗组CM_5 ST段/心率斜率减少,而对照组不变或增大,两组有显著性差异,ST段下移及常规分级运动试验其它指标在两组间无显著差异。表明CM_5ST段/心率斜率是一个敏感的心肌缺血指标。     

Potential contribution of nuclear factor-kappaB to cerebral vasospasm after experimental subarachnoid hemorrhage in rabbits.

Nuclear factor-kappaB (NF-kappaB) plays a key role in inflammation, which is involved in the development of cerebral vasospasm after subarachnoid hemorrhage (SAH). In the present study, we assessed the potential role of NF-kappaB in regulation of cerebral vasospasm. Nuclear factor-kappaB DNA-binding activity was measured in cultured vascular smooth muscle cells (VSMCs) treated with hemolysate and pyrrolidine dithiocarbamate (PDTC, 80 micromol/L), an inhibitor of NF-kappaB. Forty-two rabbits were divided into three groups: control, SAH, and PDTC groups (n=14 for each group). The caliber of the basilar artery was evaluated. Nuclear factor-kappaB DNA-binding activity and the gene expression levels of cytokines and adhesion molecules in the basilar artery were measured. Immunohistochemical study was performed to assess the expression and localization of tumor necrosis factor (TNF)-alpha, intercellular adhesion molecule (ICAM)-1, and myeloperoxidase (MPO). It was observed that NF-kappaB DNA-binding activity was significantly increased by treatment with hemolysate in cultured VSCMs, but this increase was suppressed by pretreatment with PDTC. Severe vasospasm was observed in the SAH group, which was attenuated in the PDTC group. Subarachnoid hemorrhage could induce increases of NF-kappaB DNA-binding activity and the gene expression levels of TNF-alpha, interleukin (IL)-1 beta, ICAM-1, and vascular cell adhesion molecule (VCAM)-1, which were reduced in the PDTC group. Immunohistochemical study demonstrated that the expression levels of TNF-alpha, ICAM-1, and MPO were all increased in the SAH group, but these increases were attenuated in the PDTC group. Our results suggest that NF-kappaB is activated in the arterial wall after SAH, which potentially leads to vasospasm development through induction of inflammatory response.     

西安市2～3岁儿童的气质结构探讨

本文采用幼儿气质问卷(TTS)对233名2～3岁入托儿童的气质结构进行了研究。结果显示:该年龄组不同性别儿童的气质结构无明显差异;本组儿童的主要气质类型为中间偏易型(I一E),其次为容易抚育型(E);同西方(美国纽约)儿童比较,我国儿童的气质结构有一定特征。     

免疫荧光法与 Giemsa 氏法检测沙眼衣原体的比较

本文用免疫荧光法与 Giemsa 氏法检测明显沙眼患者,两法阳性率相差无几。但在临床肉眼观察认为正常时,其阳性率分别为24.77%及11.58%(P<0.05),差异有显著意义,表明免疫荧光法检测早期沙眼较 Giemsa 氏法敏感。临床认为正常眼,经实验室检查25.56%为阳性,说明开展沙眼的实验室诊断是必要的。