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991.
Wei-Hua Dong Qin Li Xiao-Yan Zhang Qing Guo Huizheng Li Tian-Yun Wang 《International journal of clinical and experimental pathology》2015,8(9):11524-11530
Background: Vincristine (VCR) is a chemical that is widely used in tumor therapy. While long-term use can make tumor cells resistant to VCR, the underlying mechanisms of this resistance are still unclear. Objective: This study aimed at investigating the role of microRNA (miRNA) in colon cancer drug resistance. Methods: HCT-8 colon carcinoma cells were cultured and treated with different VCR concentrations to establish an HCT-8/VCR resistant cell line. Whole-genome screens, HiSeq 2500 sequencing, and bioinformatics methods were used to detect and analyze differences in miRNA expression between the drug-resistant HCT-8/VCR cells and non-resistant HCT-8 cells. Differential expression profiles of miRNAs were constructed based on sequencing result. Results: The HCT-8/VCR resistant colon carcinoma cell line was established. With regard to the difference in drug resistance between HCT-8/VCR and HCT-8 cells, 24 miRNAs showed statistically significant differences in their expression (fold change > 4), of which 17 were up-regulated. Seven miRNAs were down-regulated. Conclusion: As abnormal expression of miRNAs was associated with VCR resistance of colon carcinoma cells, differences in miRNA expression may play a key role in VCR resistance of colon cancer cells. 相似文献
992.
Minghao Kong Jing Mao Bijun Luo Zonghao Qin 《International journal of clinical and experimental pathology》2015,8(9):10929-10934
Objective: This study aimed to investigate the expression and role of Nrf2 in the acute lung injury (ALI) of mice. Methods: A total of 60 BABL/c mice were randomly divided into 2 groups: ALI group and control group. In ALI group, ALI was introduced by injection of LPS. Immunohistochemistry was performed to detect Nrf2 expression in the lung; Western blot assay was employed to detect the expression of Nrf2 in the lung homogenate; ELISA was conducted to detect the expression of Nrf2 in the lung homogenate and BALF. Results: As compared to control group, ALI mice had a high Nrf2 expression in the lung as shown in immunohistochemistry, and the Nrf2 expression in the lung homogenate and BALF also increased markedly (P<0.05). Conclusion: The Nrf2 expression increases in the lung and BALF of ALI mice, suggesting that Nrf2 is involved in the inflammation during ALI and may serve as a new target in the therapy of ALI. 相似文献
993.
目的:探讨功能性语音障碍患者应用行为疗法矫治异常舌根音,提高舌根音清晰度的方法。方法:对12例功能性异常舌根音患者运用行为疗法进行语音治疗,收集治疗前后的语音资料进行对比评估。结果:患者舌根音的语音清晰度由治疗前的52.67±11.49提高到治疗后的77.67±6.11,差异具有统计学意义(P<0.05)。结论:应用行为疗法在矫治功能性异常舌根音,可达到提高语音清晰度,改善语音功能的目的。 相似文献
994.
肺诺卡菌病胸部CT特征 总被引:1,自引:0,他引:1
目的 探讨肺诺卡菌病的胸部CT特点。方法 回顾性分析经病原学培养确诊的8例肺诺卡菌病患者的胸部CT表现、临床资料。结果 8例患者中,5例为单纯肺诺卡菌病,3例为播散型肺诺卡菌病。肺部病变以斑片、实变影最为多见(n=8),其次为结节影(n=5)、空洞影(n=4)、团块影(n=2)。4例合并胸腔积液或积脓,5例肺内病灶区域胸膜增厚,3例合并纵隔、肺门淋巴结肿大,1例合并气胸、1例合并心包积液。结论 肺诺卡菌病的胸部CT表现多样,常合并四肢或躯干皮肤或皮下软组织感染;当肺部病灶发展迅速,出现实变、结节、空洞、肿块等多样病灶时,应考虑肺部诺卡菌感染的可能。 相似文献
995.
Jing Quan Yu-jie Gao Zai-lin Yang Hui Chen Jing-rong Xian Shuai-shuai Zhang Qin Zou Ling Zhang 《International journal of medical sciences》2015,12(1):17-22
Objective: The aim of this study was to quantify the copies of circulating nucleophosmin (NPM) mutations DNA in the plasma of patients with acute myeloid leukemia (AML) and to explore the association of circulating NPM mutation levels with clinical characteristics.Design and Methods: The presence of NPM mutations in 100 Chinese patients newly diagnosed with AML were identified by RT-PCR and sequencing analysis. Copies of circulating NPM mutation A (NPM mut.A) DNA in the plasma of mutation-positive cases were quantified by real-time quantitative PCR (qRT-PCR). Furthermore, the association of circulating NPM mutation levels and clinical characteristics was analyzed.Results:
NPM mutations were identified in 37 of the 100 patients and all cases were NPM mut.A. The circulating NPM mut.A levels ranged from 0.35×108 copies/ml to 6.0×108 copies/ml in the 37 mutation-positive cases. The medium and quartile M (P25, P75) of the circulating NPM mut.A levels in patients classified as M2, M4 and M5 morphological subtypes were 1.35×108 (0.76×108, 1.91×108) copies/ml, 1.81×108 (1.47×108, 2.2×108) copies/ml and 2.50×108 (2.42×108, 3.05×108) copies/ml, respectively. Circulating NPM mut.A levels were significantly higher in patients with the M5 subtype of AML compared to patients with the M2 and M4 subtypes (p=0.000, p=0.046). In addition, circulating NPM mut.A copies were significantly associated with a higher white blood cell count, platelet count and bone marrow blast percentage (p<0.05).Conclusion: Our results suggest that circulating NPM mutations DNA assay serves as a complementary to the routine investigative protocol of NPM-mutated leukemia. 相似文献
996.
Tao Qin Yinyan Yin Lulu Huang Qinghua Yu Qian Yang 《Clinical and Vaccine Immunology : CVI》2015,22(4):421-429
Influenza whole inactivated virus (WIV) is more immunogenic and induces protective antibody responses compared with other formulations, like split virus or subunit vaccines, after intranasal mucosal delivery. Polyethyleneimine (PEI), an organic polycation, is widely used as a reagent for gene transfection and DNA vaccine delivery. Although PEI recently has demonstrated potent mucosal adjuvant activity for viral subunit glycoprotein antigens, its immune activity with H9N2 WIV is not well demonstrated. Here, mice were immunized intranasally with H9N2 WIV combined with PEI, and the levels of local respiratory tract and systemic immune responses were measured. Compared to H9N2 WIV alone, antigen-specific IgA levels in the local nasal cavity, trachea, and lung, as well as levels of IgG and its subtypes (IgG1 and IgG2a) in the serum, were strongly enhanced with the combination. Similarly, the activation and proliferation of splenocytes were markedly increased. In addition, PEI is superior as an H9N2 WIV delivery system due to its ability to greatly increase the viral adhesion to mucosal epithelial cells and to enhance the cellular uptake and endosomal escape of antigens in dendritic cells (DCs) and further significantly activate DCs to mature. Taken together, these results provided more insights that PEI has potential as an adjuvant for H9N2 particle antigen intranasal vaccination. 相似文献
997.
通过模拟临床使用一次性输液器输注青霉素钠注射液,对一次性使用输液器中可沥滤物进行分析。研究中使用了两个不同厂家A、B两种型号的一次性使用输液器,通过高效液相色谱分析发现在输注青霉素钠时并未发生药物吸附,且对有关物质研究也证实没有迁移出新的物质。但通过气相色谱-质谱分析,流经A、B两种型号输液器的药液中均检出环己酮和DEHP,A型输液器中还检出了少量的异辛醇。这些可沥滤物主要是由输液器的生产过程中引入的,且其含量均远低于人体安全摄入量,因此,本研究表明临床治疗中使用一次性输液器输注青霉素钠存在较低风险 相似文献
998.
建立了一种气相色谱法测定聚乳酸原料中封端剂十二醇残留的分析方法。着重研究了测定方法及样品的预处理方法。实验结果显示,采用DB-624(30m×0.530mm×3.0μm)毛细管柱,氢火焰离子检测器,进样口温度250℃,检测器温度260℃,程序升温(起始温度60℃,维持3 min,以30℃/min的速率升温至250℃,维持10min),十二醇的回归方程为A=2717.3C-11.646(γ=0.9998),线性范围为0.01~1.01mg/m L,平均回收率为99.21%(n=9),相对标准偏差1.55%,检测限为0.0005 mg/m L,定量限为0.001mg/m L。本实验所建立的方法能达到定量检测的目的,将该方法应用于聚乳酸原料中封端剂十二醇残留量的测定,结果可靠。 相似文献
999.
王琴 《国际病理科学与临床杂志》2015,(5)
目的:探讨早期康复护理措施对高血压脑出血颅内血肿清除术患者的应用效果。方法:回顾性分析我院2010年3月~2014年3月收治的120例高血压脑出血颅内血肿术患者临床资料,其中,自2010年3月~2012年3月收治的60例患者采用了常规护理措施,记为对照组;自2012年4月至2014年3月收治的60例患者则采取了早期康复护理措施,记为观察组。比较两组患者的护理效果。结果:护理干预后,观察组患者的MBI、ESS及GCS评分均较对照组患者出现了明显改善,组间差异具有统计学意义,观察组患者术后并发症的发生率明显低于对照组患者,组间差异具有统计学意义。结论:早期康复护理措施对高血压脑出血颅内血肿清除术患者具有十分重要的意义,可有效改善患者的神经功能,促进患者日常生活能力的改善,从而改善患者的生活质量,促进患者的预后恢复,值得临床参考。 相似文献
1000.
BackgroundThe pathogenesis of breast cancer remains unclear.AimsTo investigate the pathogenesis of breast cancer through targeted metabolomics of amino acids components in serum of patients with breast cancer.MethodsPatients with breast cancers were enrolled in our hospital between year January 1st, 2013 and December 31st, 2014. Targeted analysis of amino acids was performed using ESI-QTOF-MS instrument. In vitro experiment was performed to determine the influence of tryptophan towards interleukin-10 (IL-10) secretion by CD4+ T cell.ResultsTargeted metabolomics of amino acids showed that the level of tryptophan significantly (p<0.05) increased in patients with breast cancer. Furthermore, the biological function of tryptophan was determined through determining the influence of tryptophan towards IL-10 secretion using in vitro method. The addition of tryptophan (100 uM) in the cell medium can significantly inhibited the secretion of IL-10 by CD4+ T cells, as indicated by the mRNA level and protein concentration.ConclusionThe inhibition of IL-10 secretion by CD4+ T cells is a potential pathogenesis of breast cancer. 相似文献