环丙氧苷诱导转导有外源HSV—tk基因的C6胶质瘤细胞的 …

目的 观察单纯疱疹病毒胸苷激酶基因（ＨＳＶ－ｔｋ）－环丙氧苷（ＧＣＶ）系统在胶质瘤基因治疗过程中,ＧＣＶ能否诱导转导有ＨＳＶ－ｔｋ基因的胶质瘤细胞凋亡。方法 采用光镜、电镜和荧光显微镜观察转导有ＨＳＶ－ｔｋ基因的大鼠Ｃ６／ｔｋ胶质瘤细胞形态学的改变,以末转导ＨＳＶ－ｔｋ基因的Ｃ６细胞作为对照,并以流式细胞术,ＤＮＡ凝胶电流分析进一步验证细胞凋亡的发生。结果 在５μｇ／ｍｌ ＧＣＶ作用７２ｈ后,Ｃ６     

喉癌组织恶性度与颈淋巴结转移关系

采用五因素评点法对４０例喉鳞癌进行组织学和临床方面的研究。结果表明，组织恶性度与Ｔ分期无相关性，浸润方式弥散及恶性度高（评分≥１１）的肿瘤发生颈淋巴结转移率显著高于有完整肿瘤边界者及恶性度低（评分≤１０）者。提示喉癌组织恶性度在预测颈淋巴结转移上有实用价值，可临导临床治疗方案的选择。     

突发性聋与铁代谢障碍疾病关系的临床调查

对１９７９年７月至１９９６年６月的突发性聋发病情况进行前瞻性研究与回顾性分析，探讨突聋与铁代谢障碍疾病的关系，前瞻性研究的对象分为铁代谢障碍疾病组２１８例，血液病组２１５例和正常对照组４８５０例；回顾性分析为１７年间资料完整的４２９例突聋患者史的代谢障碍疾病及其他血液病的发病情况。结果表明，铁代谢障碍疾病组的突聋调整年发病率显著高于血液病组以及正常对照组，４２９例突聋患者的无贫血缺铁，缺铁性人同血     

CO2激光镫骨部分切除术

For the efficacy and safety of partial stapedectomy, Co2 laser was used in 44 patients with otosclerosis. Postoperative follow-up (3-12 months) demonstrated that the average hearing gain was 31.7 +/- 8.2 dB, and air-bone conduction gap was within 11.2 +/- 6.6 dB. There was no significant hearing change at 4.000 Hz. The advantages of CO2 laser technique are summarized as follows: convenience for cutting posterior crus, less time needed for penetration of thick footplate or removal of occlusive sclerotic plaque in oval window niche, avoidance of footplate floating, rapid coagulation of residual stapedial artery and absence of instrumentation difficulties in narrow spaces. Our results indicate that CO2 laser provides essential benefits in stapedectomy for otosclerosis.     

喉癌下咽癌患者一级亲属染色体对致突变物的敏感性研究

Several investigations revealed that lymphocytes from patients with head and neck cancers showed a high sensitivity to mutagens. However, whether lymphocytes from healthy blood relatives of the patients were sensitive to mutagen or not had not been reported. This study was aimed to investigate the unknown problem. Mutagen sensitivity was investigated in 116 laryngeal and 38 hypopharyngeal cancer patients, 107 healthy controls, and 30 blood relatives of laryngeal or hypopharyngeal cancer patients. Chromosome damage was measured by enumerating mutagen-induced chromatid breaks, the average number of chromatid breaks per cell (b/c value) was used as marker which reflects variable host susceptibility to the action of environmental carcinogens. The b/c values were 0.61 +/- 0.27, 0.66 +/- 0.31, 0.28 +/- 0.12, and 0.45 +/- 0.26 for patients with laryngeal cancer, hypopharyngeal cancer, healthy controls, and blood relatives respectively. A significant difference of b/c value was found between patients and controls (P < 0.01), but not between patients and their blood relatives (P > 0.05). Our findings suggest that genetic predisposition plays an important role in the eitiology of laryngeal and hypopharyngeal cancers. Both patients with laryngeal or hypopharyngeal cancers and their blood relatives should be considered as in high cancer risk.     

庆大霉素致豚鼠前庭毛细胞破坏后再生及功能恢复

为探讨哺乳动物前庭毛细胞破坏后能否再生及前庭功能能否恢复，以豚鼠为研究对象。实验组动物每日给予庆大霉素皮下注射，连续１０天，对照组动物给予等量生理盐水。于停药后的第２、４、１２和２４周分别处死动物，扫描电镜观察发现，停药２周椭圆囊和壶腹嵴毛细胞破坏最明显的区域可见到不成熟的毛细胞纤毛丛，至停药２４周后继续存在。与对照组相比，停药１２周毛细胞密度下降明显（Ｐ〈０．０１），停药后２４周时毛细胞密度较１     

电刺激蝶腭神经节对鼻粘膜血流的调控作用

为了解蝶腭神经节在鼻粘膜血流调节中的作用，应用激光多普勒血流仪观察刺激蝶腭神经节对猫鼻粘膜血流变化的调控作用，结果表明，电刺激一侧蝶腭神经节可以引起双侧鼻粘膜血管扩张，血流增加，并产生喷嚏反应，这种反应依电刺激的强度，频率而异，且与刺激时间有关。     

自适应滤波提取耳蜗微音器电位初探

为解决表面电极引导耳蜗微音器电位的结果中存在刺激伪迹信号的状况，探讨了用自适应滤波处理抵消刺激伪迹信号提取的方法，结果发现：正常耳的ＣＭ较同侧表面电极引导的波形有一定的滞后时间，２ｋＨｚ以上的神经动作电位波形分化明显；３１耳极重度感音神经性聋和１１耳中度或中重度感音神经性聋耳各频率的ＣＭ消失，９耳极重度感音神经性聋和２９中度和中重度感音神经性聋各频率存在较好的ＣＭ波形。提示：不同程度的感音神经性聋     

应用声反射鼻测量计对鼻腔最小横截面积及其位置的测定

为了确定不同年龄组鼻腔最小横截面积及其距前鼻孔的距离，应用声反射鼻测量计对１３５５名健康儿童，少年及成人（３￣８６岁）进行检测，并对儿童、少年的ＭＣＡ、ＤＣＡ值与年龄相关性进行初步探讨。