全文获取类型
收费全文 | 34997篇 |
免费 | 2659篇 |
国内免费 | 95篇 |
专业分类
耳鼻咽喉 | 334篇 |
儿科学 | 1046篇 |
妇产科学 | 903篇 |
基础医学 | 5348篇 |
口腔科学 | 473篇 |
临床医学 | 4997篇 |
内科学 | 6171篇 |
皮肤病学 | 700篇 |
神经病学 | 3911篇 |
特种医学 | 808篇 |
外科学 | 3201篇 |
综合类 | 276篇 |
现状与发展 | 1篇 |
一般理论 | 33篇 |
预防医学 | 3727篇 |
眼科学 | 607篇 |
药学 | 2287篇 |
中国医学 | 42篇 |
肿瘤学 | 2886篇 |
出版年
2024年 | 37篇 |
2023年 | 278篇 |
2022年 | 428篇 |
2021年 | 891篇 |
2020年 | 595篇 |
2019年 | 924篇 |
2018年 | 1100篇 |
2017年 | 855篇 |
2016年 | 938篇 |
2015年 | 971篇 |
2014年 | 1373篇 |
2013年 | 1772篇 |
2012年 | 2800篇 |
2011年 | 2883篇 |
2010年 | 1563篇 |
2009年 | 1420篇 |
2008年 | 2426篇 |
2007年 | 2497篇 |
2006年 | 2255篇 |
2005年 | 2350篇 |
2004年 | 2096篇 |
2003年 | 1957篇 |
2002年 | 1836篇 |
2001年 | 243篇 |
2000年 | 137篇 |
1999年 | 289篇 |
1998年 | 392篇 |
1997年 | 321篇 |
1996年 | 236篇 |
1995年 | 241篇 |
1994年 | 191篇 |
1993年 | 179篇 |
1992年 | 119篇 |
1991年 | 107篇 |
1990年 | 89篇 |
1989年 | 96篇 |
1988年 | 79篇 |
1987年 | 70篇 |
1986年 | 60篇 |
1985年 | 52篇 |
1984年 | 71篇 |
1983年 | 56篇 |
1982年 | 59篇 |
1981年 | 66篇 |
1980年 | 66篇 |
1979年 | 32篇 |
1978年 | 31篇 |
1977年 | 37篇 |
1976年 | 29篇 |
1974年 | 29篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
111.
Chapiro E Feldmann D Denoyelle F Sternberg D Jardel C Eliot MM Bouccara D Weil D Garabédian EN Couderc R Petit C Marlin S 《European journal of human genetics : EJHG》2002,10(12):851-856
Hearing impairment is the most frequent sensory defect in children, with a genetic basis in about 50% of cases. Several point mutations and deletions in mitochondrial DNA (mtDNA) have been identified in non-syndromic sensorineural hearing loss (NSSNHL). Beside the frequent A1555G mutation, a number of mutations in tRNAs have been reported recently, but their incidence remains unknown. We identified the T7511C mutation in the tRNASer(UCN) gene in two French families with isolated deafness. Maternal transmission was obvious in both. The 15 patients with hearing impairment exhibited a variable disease phenotype in terms of onset, severity, and progression. T7511C was present in all the patients screened. Homoplasmic and heteroplasmic levels were observed and did not correlate with the severity of the disease. T7511C was also present in 12 hearing offspring of the oldest deaf mothers, confirming the existence of modulatory factors. Our data suggest that this mtDNA mutation should be screened for in all cases of familial NSSNHL compatible with maternal transmission. 相似文献
112.
Cindy Johnston Stephan Eliez Jennifer Dyer‐Friedman David Hessl Bronwyn Glaser Christine Blasey Annette Taylor Allan Reiss 《American journal of medical genetics. Part A》2001,103(4):314-319
There have been contradictory findings in the fragile X (fraX) literature about possible neurocognitive and psychological symptoms due to the fraX premutation (pM). The purpose of the present study was to investigate the relationship between CGG repeat length and neurobehavioral functioning in carriers of the fraX pM. Eighty‐five female carriers of the pM with allele sizes ranging from 59–166 were administered a comprehensive IQ test (WAIS‐III) and completed a questionnaire designed to measure psychopathology (Symptom Checklist (SCL)‐90‐R). No relationship between allele size and cognition was identified. A significant negative relationship between allele size and age was found, as well as a positive relationship between allele size and depression. Follow‐up analyses separating small and large allele sizes (below and above 100 CGG repeats) indicated that individuals with larger allele sizes scored significantly higher on the Interpersonal Sensitivity and Depression subscales of the SCL‐90‐R. Despite the limitation of few individuals with high CGG repeat lengths, our findings suggest that females with larger premutated alleles (≥ 100 repeats) display some clinical manifestations of fraX syndrome. © 2001 Wiley‐Liss, Inc. 相似文献
113.
The anatomical connections of the pregeniculate complex (PrGC) with components of the visual-ocular motor system suggested its contribution to ocular motor behavior. Subsequent studies reported saccade-related activity in the primate PrGC. To determine its contribution, we characterized pregeniculate units (n = 128) in alert macaques during ocular motor tasks and visual stimulation. We found that 36/109 saccade-related units exhibited postsaccadic bursts or pauses in tonic discharge for saccades of any amplitude or direction. In contrast to previous results, 46/109 responses preceded or coincided with the saccade, while 47/109 responses were directionally tuned. Pregeniculate units were modulated not only in association with saccades (109/128) but also with smooth eye movements and visual motion (20/128) or eye position (23/128). Multiple ocular motor signals were recorded from 19% of the units, indicating signal convergence on individual neurons. Visual responses were demonstrated in 51% of PrGC units: visual field illumination modulated the resting discharge of 33 units; the responses of 37 saccade-related units and all 23 position-dependent units were modulated by visual stimulation. Early saccadic activity in the PrGC suggests that it contributes more to gaze than postsaccadic modulation of visual or ocular motor activity. The patterns of saccadic responses and the modulation of PrGC activity in association with a variety of visual-ocular motor behaviors suggest its potential role as a relay between the parietal cortex and elements of the brain stem ocular motor pathways, such as the superior colliculus and pretectal nucleus of the optic tract. 相似文献
114.
Interobserver variability in determining MIB-1 labeling indices in oligodendrogliomas 总被引:1,自引:0,他引:1
Prayson RA Castilla EA Hembury TA Liu W Noga CM Prok AL 《Annals of diagnostic pathology》2003,7(1):9-13
Several studies have shown that MIB-1 labeling indices correlate well with tumor grade and prognosis in a variety of tumor types. Several factors are responsible for some degree of variability in the determination of labeling indices. Interobserver variability is one of the factors often cited as responsible for this variability. A slide from each of 30 oligodendrogliomas, stained with MIB-1 antibody, was distributed to six pathologists. The same set of slides was reviewed by each individual. Each pathologist was instructed to determine a MIB-1 labeling index by evaluating 1,000 tumor cell nuclei from the area of the slide with the most staining. The labeling index record reflected a percentage of positive-staining tumor cells. Interobserver agreement was compared. MIB-1 labeling indices ranged from 0 to 45.7. Overall agreement was good (> or =0.75) with a concordance coefficient of 0.832 (confidence interval, 0.700 to 0.909). Variability was greater among tumors with higher labeling indices as compared with tumors with labeling indices closer to 0. The overall agreement of MIB-1 labeling indices, while not perfect, was good. The generally minor variability among observers may be related to differences in the area of the slide evaluated and in differing lower thresholds for interpreting positivity. Further improvement of concordance may theoretically be attainable by further training and discussion among observers. 相似文献
115.
Lovering Ruth; Middleton-Price Helen R.; O'Reilly Marie-Anne J.; Genet Sally A.; Parkar Mohammed; Sweatman Angela K.; Bradley Linda D.; Alterman Lesley A.; Malcolm Sue; Morgan Gareth; Levinsky Roland J.; Kinnon Christine 《Human molecular genetics》1993,2(2):139-141
Genetic linkage analysis has been instrumental in mapping thegene for X-linked agammaglobulinemia (XLA) to the proximal longarm of the human X chromosome, to Xq22. Due to the relativerarity of this disease the localization of the gene within Xq22has remained imprecise. We have investigated twenty-nine familiesaffected by XLA and have found no recombinants with the DXS178locus in over 30 informative meioses. DXS178 is now the mostreliable and informative locus for use in pre-natal diagnosisand carrier detection of XLA. In addition, we have identifiednew closely linked proximal and distal flanking markers forXLA, DXS442 and DXS101, respectively. These loci are separatedby 2cM, considerably reducing the extent of DNA within whichthe XLA locus can be contained. This will open up the way formore directed positional cloning efforts for the isolation ofthe XLA gene. 相似文献
116.
Anne-christine Jauneau Alexander Ischenko Alexandra Chatagner Magalie Benard Philippe Chan Marie-therese Schouft Christine Patte Hubert Vaudry Marc Fontaine 《Journal of neuroinflammation》2006,3(1):8-10
C3a and C5a anaphylatoxins are proinflammatory polypeptides released during complement activation. They exert their biological
activities through interaction with two G protein-coupled receptors named C3aR and C5aR, respectively. In the brain, these
receptors are expressed on glial cells, and some recent data have suggested that anaphylatoxins could mediate neuroprotection.
In this study, we used RT-PCR and ribonuclease protection assays (RPA) to investigate the role of anaphylatoxins on neurotrophin
expression by the human glioblastoma cell line T98G and by rat astrocytes. Our data show that for both cell types, anaphylatoxins
upregulate expression of NGF mRNA. This response depended on a G protein-coupled pathway since pre-treatment of cells with
pertussis toxin (PTX) completely blocked NGF mRNA increases. This effect was anaphylatoxin-specific since pre-incubation with
anti-C3a or anti-C5aR antibodies abolished the effects of C3a and C5a, respectively. The regulation of NGF mRNA by anaphylatoxins
was not accompanied by translation into protein expression, but there was a significant synergic effect of anaphylatoxins/IL-1b
costimulation. Our demonstration of involvement of anaphylatoxins in the NGF release process by astrocytes suggests that C3a
and C5a could modulate neuronal survival in the CNS. 相似文献
117.
118.
Molecular genetic analysis of familial early-onset Alzheimer's disease linked to chromosome 14q24.3 总被引:4,自引:2,他引:4
Cruts Marc; Backhovens Hubert; Wang Sheng-Yue; Van Gassen Geert; Theuns Jessie; De Jonghe Chris; Wehnert Anita; De Voecht Joke; De Winter Goedele; Cras Patrick; Bruyland Marc; Datson Nicole; Weissenbach Jean; Dunnen Johan T.den; Martin Jean-Jaques; Hendriks Lydia; Van Broeckhoven Christine 《Human molecular genetics》1995,4(12):2363-2371
Genetic linkage studies have indicated that chromosome 14q24.3harbours a major locus for early-onset (onset age <65 years)Alzheimer's disease (AD3). Positional cloning efforts have identifieda novel gene S182 or presenilin 1 as the AD3 gene. We have mappedS182 in the AD3 candidate region between D14S277 and D14S284defined by genetic linkage studies in the two chromosome 14linked, early-onset AD families AD/A and AD/B. We have shownthat S182 is expressed in lymphoblasts and have determined thecomplete cDNA in both brain and lymphoblasts by RT-PCR sequencing.S182 is alternatively spliced in both brain and lymphoblastswithin a putative phosphorylation site located 5' in the codingregion. We identified two novel mutations, Ile143Thr and Gly384Alalocated in, respectively, the second transmembrane domain andin the sixth hydrophilic loop of the putative transmembranestructure of S182. As families AD/A and AD/B have a very similarAD phenotype our observation of two mutations in functionallydifferent domains suggest that onset age and severity of ADmay not be very helpful predictors of the location of putativeS182 mutations. 相似文献
119.
Dzien A Pfeiffer KP Dzien-Bischinger C Hoppichler F Lechleitner M 《Acta medica Austriaca》2003,30(2):51-54
Obesity is a serious health problem in industrialized countries and is associated with a significant increase in total health care costs. Only few data are available about the costs of drug therapies in patients with an increased body weight treated under clinical routine procedures. Such data could support efforts to intensify obesity prevention and treatment programmes in order to reduce comorbidities and costs. We have evaluated body mass index (BMI), diagnosis, and medication in 3360 outpatients (2175 women and 1185 men; mean age: 56.7 +/- 17.5 years). All patients underwent physical examinations, including BMI determination, and provided a detailed record concerning medication. In 1809 patients, the percentage of body fat content was measured with a bioimpedance method (OMRON BF 302 body fat monitor). Continuous variables were compared using the t-test or Wilcoxon U-test. Frequency distributions were compared using chi-squared tests. With respect to BMI, most of the patients (n = 1793; 53 %) were overweight or obese, 1349 (40 %) showed a normal BMI and 218 (7 %) a low BMI. The majority of cardiovascular (61 %), rheumatological (61.1 %) and metabolic (60.4 %) medication was administered to overweight and obese patients. Parallel findings could be obtained by analysing the percentage of body fat and the frequency of medication. Overall, 82.5 % of all medication was given to patients with a body fat content >20 %. Our results support the importance of weight-reduction programmes in order to prevent an overall increase in the costs of medication as a consequence of overweight and obesity. 相似文献
120.
Joel J. Hillhouse Arthur W. Stair III Christine M. Adler 《Journal of behavioral medicine》1996,19(6):543-561
Sunbathing and sunscreen use, as well as related intentions, attitudes, beliefs, and knowledge, were assessed in 90 Southern Appalachian undergraduates. A large majority (75%) reported sunbathing, with more than half reporting sun lamp use. A slight majority (56.7%) reported some use of sunscreen. Subjects reporting an intention to tan spent more time sunbathing, both outside and under a sun lamp. Individuals reporting a sun protection intention had lighter tans and spent less time sunbathing. Sunbathing was predicted by perceptions of sunbathing as relaxing, while sun lamp use was predicted by more positive views of suntatns. Sunscreen use was predicted by more positive sun protection attitudes and less negative sunscreen attitudes. 相似文献