阿尔茨海默症患者主要照顾者悲伤与照顾负担的相关性研究

[目的]探索阿尔茨海默症患者主要照顾者悲伤与照顾负担的相关性。[方法]采用Marwit-Meuser照顾者悲伤量表(MM-CGI)和照顾负担量表(CBI),选择2018年3月至2019年3月在开封市某三级甲等医院就诊的80名阿尔茨海默症患者,对其主要照顾者的悲伤和照顾负担情况进行调查。[结果]阿尔茨海默症照顾者悲伤总分为(56.46±7.92)分,照顾负担总分为(61.99±12.92)分,两者均处于中等偏高水平。阿尔茨海默症患者主要照顾者悲伤程度与照顾负担呈正相关。[结论]医护人员应探索积极、有效的干预措施应对阿尔茨海默症患者主要照顾者的悲伤情绪及其照顾负担。     

牙周膜干细胞的研究进展

牙周病是一种由菌斑微生物引起的慢性感染性疾病,可引起牙周支持组织的破坏和丧失,最终导致牙齿松动脱落。牙周病治疗的最终目标是修复和重建受损的牙周支持组织。从牙周膜中分离获取的间充质干细胞具有成体干细胞的特性及多重分化潜能,可以分化为骨组织和牙周支持组织等多种类型的组织,这对牙周组织修复再生和牙周组织工程具有重大意义,因而备受关注。本文就牙周膜干细胞、牙周膜干细胞的生物学特性、牙周膜干细胞的影响因素及其调控机制等研究进展作一综述。     

Role of PERK/eIF2α/CHOP Endoplasmic Reticulum Stress Pathway in Oxidized Low-density Lipoprotein Mediated Induction of Endothelial Apoptosis

Objective PERK/eI F2α/CHOP is a major signaling pathway mediating endoplasmic reticulum(ER) stress related with atherosclerosis.Oxidized LDL(ox-LDL) also induces endothelial apoptosis and plays a vital role in the initiation and progression of atherosclerosis.The present study was conducted to explore the regulatory effect of ox-LDL on PERK/e IF2α/CHOP signaling pathway in vascular endothelial cells.Methods The effects of ox-LDL on PERK and p-e IF2α protein expression of primary human umbilical vein endothelial cells(HUVECs) were investigated by Western blot analysis.PERK gene silencing and selective eI F2α phosphatase inhibitor,salubrinal were used to inhibit the process of ox-LDL induced endothelial cell apoptosis,caspase-3 activity,and CHOP mR NA level.Results Ox-LDL treatment significantly increased the expression of PERK,PERK-mediated inactivation of e IF2α phosphorylation,and the expression of CHOP,as well as the caspase-3 activity and apoptosis.The effects of ox-LDL were markedly decreased by knocking down PERK with stable transduction of lentiviral sh RNA or by selective eI F2α phosphatase inhibitor,salubrinal.Conclusion This study provides the first evidence that ox-LDL induces apoptosis in vascular endothelial cells mediated largely via the PERK/eI F2α/CHOP ER-stress pathway.It adds new insights into the molecular mechanisms underlying the pathogenesis and progression of atherosclerosis.     

股骨头负重区松质骨的压缩力学特性

背景松质骨力学特性在代谢性疾病、骨折、关节退行性变的内在发生规律中具有意义.目的了解松质骨的生物力学特性.设计以股骨标本为单一样本的实验观察.单位上海第二医科大学附属第九人民医院骨科生物力学实验室.对象2001-05/07完成于在上海第九人民医院骨科生物力学实验室,选取19具男性尸体股骨头.方法取19具新鲜青年(30岁左右)尸体股骨.对股骨头负重区的松质骨进行压缩测试.主要观察指标标本的屈服载荷、极限应力、极限应变、能量值、弹性模量.结果测算松质骨各项压缩数据,其中屈服载荷的平均值为(410.64±190.29)N,极限应力为(8.69±3.75)MPa,极限应变为(10.84±6.58)%,能量值为(2.54±1.89)J,弹性模量为(40.77±32.12)MPa.加载初期松质骨呈弹性变形,此后横截面增大抗压能力增强.结论试验表明松质骨力学性能主要是缓解外力冲击加压.