Chromosomal imbalances in lung adenocarcinomas with or without mutations in the epidermal growth factor receptor gene

Background and objective: Epidermal growth factor receptor (EGFR) mutations are common in lung adenocarcinomas of Asian patients, implying a good response to treatment with the EGFR tyrosine kinase inhibitors, gefitinib and erlotinib. However, the distinct chromosomal imbalances between lung adenocarcinomas with and those without EGFR mutations have not been fully elucidated. Methods: Seventy‐seven patients of surgically resected lung adenocarcinoma were analysed for the EGFR exon 19 deletion and the L858R mutation, using mutant‐enriched PCR, and for chromosomal imbalance alterations using comparative genomic hybridization. Results: EGFR mutations were detected in 42 (54.5%) patients, including 22 with the exon 19 deletion and 20 with the L858R mutation. The mean number of chromosomal arms with imbalance alterations was significantly higher in tumours with EGFR mutations than in those lacking these two mutations. The minimal regions with gain on 1q23–q31, 6p12–p21.1 and 7q11.2, and loss on 3p21, 8p22–p23, 9q33, 10q25 and 13q13, differed significantly between lung adenocarcinomas with or without EGFR mutations. However, neither EGFR mutations, nor any of the common chromosomal imbalance alterations alone, exhibited significant associations with tumour stage or disease‐specific survival of the patients. Conclusions: These results indicate that imbalance alterations at several chromosomal regions occur significantly more frequently in lung adenocarcinomas with EGFR mutations than in those without such mutations. Tumour growth‐related genes in these chromosomal regions should be further investigated to improve our understanding of the common genetic alterations in lung adenocarcinomas with EGFR mutations.     

脑卒中单侧忽略的康复治疗：随机对照临床研究

目的：探讨脑卒中单侧忽略的康复治疗方法。方法：60例首次脑卒中后有左侧偏瘫及单侧忽略的患者。随机分为3组，2个干预组，1个对照组。第1组使用主动向患侧转动身躯的方法假），第2组使用TR及特殊眼镜遮盖双眼右半侧视野（EP），对照组则只使用传统日常生活动作及手功能训练。评价分3个阶段进行偏身忽略、功能及认知测试。结果：4周治疗后，TR组与对照组间在FIM行动评分方面有明显差异，尤其在移位、转移、行动、上下楼梯和FIM肢体总分方面。结论：对有单侧忽略的初期脑卒中患者应在早期积极采用向患侧转身的康复治疗方法。     

麻黄碱的作用受体研究——家兔主动脉条肾上腺素能受体

麻黄碱对离体家兔主动脉条具兴奋作用,此种兴奋可被苯苄胺所阻断。高浓度的麻黄碱对苯苄胺的阻断具“自我保护”(self-protection)作用,对腎上腺素或去甲腎上腺素具“交叉保护”(cross-protection),而后二者对麻黄碱則无“交叉保护”作用。这些实驗指出,麻黄碱除作用于腎上腺素能受体外,对主动脉平滑肌尚可能有其它作用受体。     

昆明山海棠根部水提液诱导HL-60细胞凋亡及对c-Myc基因表达的调控

目的 研究昆明山海棠Tripterygium hypoglaucum(Celastraceae)(THH)根部水提液对早幼粒白血病HL-60细胞凋亡的诱导作用及机制。方法 通过特征性的形态学观察，Annexin—V标记，以及流式细胞仪检测中次G1峰的形成确定THH的诱导细胞凋亡作用；应用Western Blotting研究THH对c—Myc蛋白表达的影响。结果 THH根部水提液可诱导早幼粒白血病HL-60细胞凋亡。在浓度高于18μg／mL时，THH对HL-60细胞的诱导凋亡作用呈现浓度与时间的相关性。在THH处理2～4h后，癌基因蛋白c—Myc表达降低99％以上。结论 THH抑制了c—Myc蛋白的翻译或后翻译过程，从而降低了c—Myc在细胞周期运转中的作用，导致大量细胞凋亡．     

Molecular spectra of acrylamide—induced mutation at hprt locus in human promyelocytic leukemia HL—60 and NB4.cell lines

The genotoxicity of acrylamide was investigated by methods of single cell clone culturing, two-way screening count, multiplex PCR amplification and electrophoresis technique. Acrylamide only showed clear mutagenesis until dose raised 700 mg*L-1 in HL-60 cells. The most frequent spontaneous mutation were point mutation (≥90.0%) and acrylamide-induced mutation mainly included partial deletion and point mutation (respectively 40.0%～66.7%/33.3%～60.0%). Total gene deletion wasn't discovered in both of cells. There were deletion mutation in all exons of hprt gene(except 7/8 exon), and toward the 3' end of the hprt gene. The most frequent acrylamide-induced mutation were point mutation and single exon deletion (93.3%/86.1%). There were not clear difference in both of cells. The results suggest that the spectra of spontaneous and acrylamide-induced mutants were different. and the smaller changes in genetic structure have something to do with mechanism.     

从人肝细胞文库中克隆Fas cDNA

为获取人FascDNA ,以探索Fas的功能作用 ,本文采用高滴度Dig -FascDNA探针噬菌斑影印杂交筛选技术 ,从人肝细胞cDNA文库 (HL -cDNA)中获得多个FascDNA克隆     

Ureteral obstruction caused by a duplicated anomaly of inferior vena cava

Developmental anomalies of inferior vena cava are a rare cause of ureteral obstruction. We report a case that presented with right upper ureteral obstruction that radiologically simulated a retrocaval ureter. An aberrant vessel, which caused obstruction of the right ureter was identified at operation and surgical relief of ureteral obstruction was performed. Inferior venocavography was performed postoperatively and disclosed an unusual incomplete duplication of inferior vena cava. Our findings suggested that ureteral obstruction by incompletely duplicated anomaly of the inferior vena cava should be included in the differential diagnosis of extrinsic ureteral obstruction.