Significance of Rosiglitazone Inhibiting TLR4 Expression in Partial Hepatic Ischemia/Reperfusion of Mice

The effect of rosiglitazone as the ligand of peroxisome proliferator-activated receptor γ (PPARγ) inhibiting the TLR4 expression in ischemic lobes in partial hepatic ischemia/reperfusion injury (IRI) in BABL/C mice and the action of rosiglitazone inhibiting the TLR4 receptor-mediated inherent immune response were investigated. The model of the mouse partial hepatic ische- mia/reperfusion injury was established. All the animals were randomly divided into 3 groups: rosiglitazone group, vehicle (dimethylsulphoxide, DMSO) group and sham operation group. The hepatic samples were collected when mice were sacrificed 0, 2, 4 and 6 h after reperfusion following 1 h ischemia to analyze the acute phase of hepatic IRI. The dynamic expression of TlR4 mRNA was de- tected quantitatively by real-time-PCR, and the levels of TNF-α, IL-10 and ALT in portal vein were determined in all groups. After restoration of blood supply, the expression of TLR4 mRNA in ischemic lobes was detected in 0, 2, 4 and 6 h after reperfusion following 1 h ischemia in rosiglitazone group and vehicle group. The most intensive expression of TLR4 mRNA was present at 4 h after reperfusion in ischemic lobes in vehicle group. As compared with vehicle group, the expression of TLR4 mRNA in ischemic lobes in rosiglitazone group was significantly decreased at 4 h after reper- fusion. The level of IL-10 in portal vein was markedly up-regulated in rosiglitazone group as compared with vehicle group. Contrarily, the levels of TNF-α and ALT in portal vein were markedly down-regulated in rosiglitazone group as compared with vehicle group at every time point in mouse partial hepatic IRI model. Rosiglitazone could alleviate the hepatic IRI by inhibiting TLR4 receptor-mediated inherent immune response.     

OCT在视网膜疾病诊断中的应用

目的：探讨光学相干断层扫描（OCT）在视网膜疾病诊断中的应用。方法：采用Zeiss-Hump hrey OCT3仪,检测2007年11月-2008年7月在本科住院的168例视网膜疾病患者。结果：168例患者中25例黄斑裂洞;11例糖尿病视网膜病变;68例黄斑变性;10例中心性视网膜炎;25例眼外伤;4例视网膜血管病（2例视网膜中央动脉阻塞,2例视网膜中央静脉阻塞,2例分支静脉阻塞）;10例视网膜脱离;15例高度近视,在OCT的检测结果中都有相应的形态改变。结论：OCT在眼科疾病的诊断、鉴别诊断、病情监测及对其发病机制的分析研究等方面均有重要的应用价值。     

医学实验动物学教学改革的实践与探讨

实验动物学是一门新兴的前沿学科,已形成独特的理论体系和学科特色。近几年来,实验动物学已成为生命科学发展的重要支撑,在高等医学教学中具有重要的地位。根据我国实验动物学的教育现状,在实验动物学教学实践中,对教学内容、教学方法和考核方式等方面进行了改革和探索,以期为建立创新型的实验动物学教学体系奠定基础。     

The Effect of Targeted Magnetic Nanopaticles on Hepatoma and the Expression of bcl-2/bax Protein

The effect of targeted magnetic nanoparticles on hepatoma and the underlying mechanism were examined. Nude mice transplanted with a human hepatoma cell line （HepG2 cells） were randomized into 5 groups, including： （1） group A, receiving normal saline, （2） group B, receiving 5-fluorouracil （5-Fu）, （3） group C, receiving magnetic nanoparticles containing 5-Fu, （4） group D, consisting of treatment with magnetic nanoparticles containing 5-Fu and inside magnetic field and （5） group E, receiving pure magnetic nanoparticles and inside magnetic field. Morphological features of transplanted tumors in mice in each group were observed under transmission electron microscope （TEM）. The expression of bcl-2/bax protein was immunohistochemically detected by SABC method. The results showed that a large number of apoptotic tumor cells were found in group B and group D under TEM. The expression of bcl-2 protein was significantly decreased and the expression of bax protein increased significantly in both group B and D as compared with those in group A, C and E （P〈0.01 for all）. The decrease in bcl-2 and the increase in bax were more in group D as compared with group B （P〈0.01）. It is concluded that the targeted magnetic nanoparticles containing 5-Fu can improve the chemotherapeutic effect of 5-Fu by decreasing bcl-2 expression, increasing bax expres- sion and inducing apoptosis of the liver cancer cells.     

参芪扶正注射液对肝癌小鼠5-FU化疗后免疫功能的影响

目的:探讨参芪扶正注射液对肝癌小鼠5-Fu化疗后免疫功能的影响.方法:建立H22肝癌荷瘤鼠模型后,采用5-FU 25 mg/kg连续5 d腹腔注射建立肝癌小鼠化疗后动物模型,然后随机分为对照组和治疗组,治疗组给予参芪扶正注射液40 mL/(kg·d)、80 mL/(kg·d)分别腹腔注射给药,连续7 d;同时对照组予生理盐水40 mL/(kg·d).末次给药后24 h采用免疫器官重量法检测胸腺指数/脾脏指数;同时采用放射免疫法测定小鼠血清中IL-2和TNF-α的含量.结果:治疗组小鼠胸腺指数、脾脏指数均高于对照组(P<0.05);治疗组小鼠血清IL-2水平显著高于对照组,而治疗组TNF-α低于对照组(P<0.05).结论:参芪扶正注射液可提高肝癌小鼠5-FU化疗后的免疫功能,临床上可试用参芪扶正注射液对肝癌化疗后免疫功能低下患者进行治疗.     

右半结肠癌D3根治术32例临床研究

目的 探讨右半结肠D3根治术的临床应用.方法 2000年5月-2006年5月,对32例右半结肠癌患者行D3根治术.手术完整切除Toldt筋膜及胰头十二指肠前筋膜,游离肠系膜上静脉外科干.根部结扎肠系膜血管,清扫N3淋巴结.肝曲癌再切除右肾前筋膜,清扫幽门下淋巴结.肿瘤侵出Toldt筋膜清扫其下腹膜下筋膜(Gerota筋膜),作右半结肠切除.结果 手术清扫淋巴结每例达15-76枚,平均28枚.术后并发症21.88%(7/32),其中切口感染3例,术后炎症性肠梗阻2例,急性左心衰及肺部感染各1例.无围手术期死亡患者.32例患者随访8-72个月,平均38个月,生存36个月以上22例,6例分别于术后8、14、18、24、25和32个月死亡.随访期间失访4例.结论 右半结肠D3根治术是减少肿瘤复发的有效术式,仔细操作并不增加手术并发症.     

源《内经》析失眠

源<内经>中有关失眠的论述内容,对睡眠的生理、病理作一剖析,认为"不寐者,因虚因实,病在阳不交阴",外邪内伤、精伤营亏皆可致病,治当补其不足,泻其有余,和调阴阳.     

恶性疟原虫对S—P抗药性流行病学及机制研究进展

疟疾是世界上危害人类健康最严重的感染性疾病之一。疟原虫尤其是恶性疟原虫对治疗药物产生抗药性是有效治疗疟疾面临的主要问题。恶性疟原虫对氯喹的高水平抗性使许多国家把磺胺多辛-乙胺嘧啶作为一线抗疟药。随着该药的使用，恶性疟原虫的抗性迅速发展。各疟区已有使用该复方治疗失败的报道。本文综述该复方抗药性流行病学的研究、抗药性产生的机制及促使抗药性产生发展的因素。     

纳络酮在重症颅脑损伤治疗中的疗效观察

目的：为了探讨早期应用纳络酮（NLX）在急性重症颅脑损伤治疗中的作用。方法：我们在两年内对收治的急性重症颅脑损伤患者随机分为治疗组（64例）和对照组（70例），对两组病人的死亡率、伤残率和痊愈率进行统计学处理分析。结果：发现NLX治疗组病人的死亡率、伤残率明显低于对照组，P〈0.05，痊愈率明显高于对照组，P〈0.05。结论：早期应用纳络酮可有效降低急性重症颅脑损伤患者的伤残率和死亡率。     

感染性腹泻病原学研究进展

感染性腹泻是一个古老而常见的疾患,是由病原微生物及其产物所引起的、以腹泻为主并广泛存在、流行于世界各地的肠道传染病,也是当今全球性重要的公共卫生问题之一。随着细胞培养和分子生物学研究的进展,特别是DNA探针及PCR技术的应用,从70年代以后,病毒感染性腹泻病原学中的地位日益重要,在细菌和寄生虫方面也同样出现了不少过去鲜为人知的新种类和型别。