山东产四种菟丝子免疫增强作用的比较研究

目的 比较山东产四种菟丝子对小鼠免疫功能、抗疲劳、耐缺氧等方面的作用。方法 选用昆明种小鼠，用山东产四种菟丝子的水、醇提取物灌胃，观察小鼠腹腔巨噬细胞吞噬功能、免疫器官重量的变化、游泳实验和耐缺氧等指标。结果 山东产四种菟丝子均可增加小鼠吞噬百分率，使幼龄小鼠的胸腺和脾脏增重，并可显著延长小鼠的游泳及缺氧存活时间。其中以菟丝子和南方菟丝子的作用较好，水提取物的作用优于醇提取物。结论 山东产四种菟丝子具有增强机体免疫功能、抗疲劳、耐缺氧作用。菟丝子中的多糖是其提高机体免疫力，增强体质的有效物质之一。     

In vitro differentiation of human adipose-derived adult stromal cells into neuron-like cells in hippocampal astrocyte conditioned medium

BACKGROUND: At present, researches on differentiating from human adipose-derived adult stromal cells (hADASC) to neuron-like cells are focus on inducing by artificial-synthetic compound solution; however, hippocampal astrocyte conditioned medium (HCAM) can induce in vitro differentiation from hADASC to neuron-like cells is still unclear. OBJECTIVE: To observe whether HCAM can induce in vitro differentiation from hADASC to neuron-like cells. DESIGN: Randomized control study. SETTING: Department of Neurology, Taixing People's Hospital; Central Laboratory, North China Coal Medical College. MATERIALS: Donor of adipose tissue was donated by female volunteers suffering from caesarean section in the department of obstetrics & gynecology in our hospital and aged 20－35 years. Adipose tissue was collected from subcutaneous tissue of abdomen during the operation. In addition, 8 male newborn Wistar rats within 24 hours with average body mass of 20 g were provided by Animal Institute of Chinese Academy of Medical Sciences. Rabbit-anti-human Nestin polyclonal antibody, rabbit-anti-human glial fibriliary acidic protein (GFAP) polyclonal antibody, rabbit-anti-human neuro-specific enolase polyclonal antibody and mouse-anti-human microtubal associated protein 2 (MAP-2) polyclonal antibody were provided by Wuhan Boster Company. METHODS: The experiment was carried out in the Central Laboratory of North China Coal Medical College from October 2004 to June 2005. hADASC was cultured with HCAM and its growth and morphological changes were observed under inverted phase contrast microscope. Immunocytochemistry, immunofluorescence and Western blotting were used to evaluate the expressions of Nestin, which was a specific sign of nerve precursor, neuro-specific enolase and MAP-2, which was a specific sign of nerve cell, and GFAP, which was a specific sign of neuroglial cells. MAIN OUTCOME MEASURES: Nestin, which was a specific sign of nerve precursor, neuro-specific enolase and MAP-2, which was a specific sign of nerve cell, and GFAP, which was a specific sign of neuroglial cells. RESULTS: On the 3rd day of culture, partial hADASC started deformation from slender shuttle-shape cells to neuron-like cells. It suggested that cells stretched out apophysis, which were mainly double-pole or multiple-pole cells. Five days later, immunohistochemical detection suggested that expression of Nestin (10.5±0.037) was found out in cells; meanwhile, expressions of GFAP (38.4±0.052) and neuro-specific enolase (NSE) (15.7±0.023) were also found out in cells; however, expression of MAP-2 was not observed. Western blot indicated that, 5 days after effect of HCAM, Nestin was found out in hADASC; meanwhile, expressions of GFAP and neuro-specific enolase were also found out; however, expression of MAP-2 was not observed. CONCLUSION: HCAM can induce the differentiation from hADASC to neuron-like cells in vitro.     

粗糙脉孢菌漆酶基因的克隆及在毕赤酵母中的初步表达

采用连续延伸PCR方法克隆到粗糙脉孢菌（Neurospora crassa）漆酶基因，并将其克隆到表达载体pPIC9k，重组质粒经线性化、电激转化Pichia pastoris KM71，部分阳性克隆的PCR结果表明：漆酶基因已整合到巴斯德毕赤酵母染色体上，重组菌经甲醇诱导后3～5d产漆酶量最高，为2-3U／mL。     

腹腔内疝的CT表现

腹腔内脏器穿过腹膜或膜突入另一腹腔内间隙称为腹腔内疝（internal abdominal hernias）。最常表现为一段小肠嵌入正常或异常的腹腔内孔道，引起急性小肠梗阻。作为疝口的结构可以是腹腔内的正常解剖结构，如裂孔、隐窝、陷窝等，     

红细胞同工酶ADA－EAP一AK_1同步电泳分型方法的建立

综合、改良了分别分型的方法，建立了红细胞同工酶ＡＤＡ一ＥＡＰ一ＡＫ１同步电泳分型方法，为ＡＤＡ，ＥＡＰ和ＡＫ１３种红细胞同工酶在法医学鉴定中更广泛的应用，提供了一种可靠、经济、实用的方法。     

对医院药事管理委员会系统工程管理的初探

本文通过对医院药事管理委员会的组织形式以及在医院药学事业中的特殊地位、工作任务、工作职责及职能效应的初步探讨,指出医院药事管理委员会在发展和建设医院药学事业中具有十三种职能、七大效应。认为加强医院药事管理委员会建设,是发展医院药学事业和保证医院药品质量的一种好方式。     

早期选择性消化道脱污染治疗大鼠重症急性胰腺炎

目的　探讨早期选择性消化道脱污染 (SDD )对大鼠重症急性胰腺炎 (SAP )的保护作用及其机理。方法　 60只大鼠制成SAP模型后 ,随机分为SAP组和SAP加SDD组 ,后者在模型建立后即行SDD ,2次 /d ,共 3d。检测对比两组 2 4,48,72h时外周动脉血和门静脉血清内毒素、肿瘤坏死因子 -α(TNF α)和白细胞介素 -1(IL 1)浓度 ,观察动物死亡率、存活时间及胰腺病理学变化。结果　SAP加SDD组各时相外周动脉及门静脉血的内毒素、TNF α和IL 1浓度 ,以及动物死亡率均显著低于SAP组 (P <0 .0 1) ;而存活时间显著高于SAP组 (P <0 .0 5 ) ;两组胰腺坏死程度评分有显著性差异 (P <0 .0 5 )。结论　早期选择性消化道脱污染可以通过清除肠道内毒素来降低SAP大鼠全身炎症反应程度 ,减轻多器官损害。     

Nitric oxide: a revolution of free radicals theory of aging

There are two kinds of free radicals in the aging process, 'bad' and 'good'. Nitric oxide is a good free radical.     

牙槽裂植骨改善唇腭裂患者鼻底塌陷畸形

分析唇腭裂患者牙槽裂植骨与改善鼻底塌陷畸形的关系。方法：对同一术者所行牙槽裂植骨术的单侧唇腭裂患者48例进行临床研究，结合X线片及面部正侧位照片探讨植骨成功与否与鼻底塌陷畸形改善的关系。结果：植骨成功组的患侧鼻底塌陷畸形的改善较未成功组有明显差异，明显高于未成功组。结论：牙槽裂植骨成功后患侧鼻底塌陷畸形可以得到明显改善。