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211.
Balakrishnan P Solomon S Mohanakrishnan J Cecelia AJ Kumarasamy N Murugavel KG Venkatakrishnan B Solomon SS Crowe SM Ganesh AK Thyagarajan SP Flanigan T Mayer KH 《Journal of acquired immune deficiency syndromes (1999)》2006,43(1):23-26
Serial measurements of absolute CD4+ T-lymphocyte counts are required to initiate and gauge response to therapy and monitor disease progression. Hence, there is an urgent need to evaluate the accuracy and validity of low-cost CD4+ T-cell count assays. Tripotassium EDTA blood specimens from HIV-infected individuals were studied using a novel flow cytometric assay (EasyCD4 assay; Guava Technologies, Hayward, CA) in comparison with standard flow cytometry (FACSCount; Becton Dickinson Immunocytometry Systems, San Jose, CA). The sensitivity, specificity value by EasyCD4 assay in enumerating absolute CD4+ T-cell counts of less than 200 cells/microL were 95% and 100%, respectively. Bland-Altman analysis showed close agreement, with the EasyCD4 assay yielding CD4+ T-cell counts a mean difference of -26 cells/microL (95% confidence interval, -96 to 44 cells/microL) higher than by flow cytometry. Our data suggest that EasyCD4 assay could be a useful alternative assay to conventional flow cytometry, may be appropriate for use in resource-limited settings. 相似文献
212.
Saini J Bagepally BS Bhatt MD Chandran V Bharath RD Prasad C Yadav R Pal PK 《Parkinsonism & related disorders》2012,18(5):477-482
IntroductionEssential tremor (ET) is a common movement disorder with motor and non-motor symptoms. We aimed to investigate the neurodegenerative changes in the brain white matter of patients with ET using Diffusion Tensor Imaging (DTI).MethodsClinical and MRI data from 20 patients (5 women and 15 men; age-38.2 ± 16.5 yrs) with ET and 17 controls (3 women and 14 men; age-40.7 ± 16.5 yrs) were collected prospectively. The DTI data were analyzed using tract based spatial statistics (TBSS) software for tract wise analysis. Further region of interest (ROI) analysis was carried out in the genu of corpus callosum, anterior limb of internal capsule (ALIC), corticospinal tract (CS), and cerebellar peduncles. Effect of tremor severity, disease duration and age of onset on DTI metrics was also studied.ResultsPatients with ET in comparison to controls showed significant (Pcorrected < 0.05) increase of mean diffusivityand radial diffusivity in right frontoparietal white matter. Axial diffusivity increase was seen in bilateral cerebral hemispheres, thalamus, brainstem and cerebellar hemisphere white matter. No significant change in fractional anisotropy of the white matter was seen. ROI analysis also revealed abnormalities in the ALIC and cerebellar peduncles. There was no correlation between the severity of white matter changes and clinical tremor severity score as well as disease duration.ConclusionsThis study provides in vivo evidence for axonal disintegration of the cerebral and cerebellar white matter fibres in patients with ET. 相似文献
213.
O'Rourke N McElrath T Baum R Camann W Tuomala R Stuebe A Kodali BS 《Anesthesia and analgesia》2007,104(5):1193-4, tables of contents